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Pavlova LN, Zhavoronkov LP, Pavlov VV, et al. Experimental Study of the Efficacy of Sodium Deoxyribonucleate Used in Combination with Co-Transplantation of Mesenchymal and Hematopoietic Stem Cells after Exposure to γ-Radiation. Bull Exp Biol Med. 2021;172(2):236-244doi: 10.1007/s10517-021-05369-z.
Pavlova, L. N., Zhavoronkov, L. P., Pavlov, V. V., Panfilova, V. V., Izmest'eva, O. S., Chibisova, O. F., Ivanov, V. L., Shegai, P. V., & Kaprin, A. D. (2021). Experimental Study of the Efficacy of Sodium Deoxyribonucleate Used in Combination with Co-Transplantation of Mesenchymal and Hematopoietic Stem Cells after Exposure to γ-Radiation. Bulletin of experimental biology and medicine, 172(2), 236-244. https://doi.org/10.1007/s10517-021-05369-z
Pavlova, L N, et al. "Experimental Study of the Efficacy of Sodium Deoxyribonucleate Used in Combination with Co-Transplantation of Mesenchymal and Hematopoietic Stem Cells after Exposure to γ-Radiation." Bulletin of experimental biology and medicine vol. 172,2 (2021): 236-244. doi: https://doi.org/10.1007/s10517-021-05369-z
Pavlova LN, Zhavoronkov LP, Pavlov VV, Panfilova VV, Izmest'eva OS, Chibisova OF, Ivanov VL, Shegai PV, Kaprin AD. Experimental Study of the Efficacy of Sodium Deoxyribonucleate Used in Combination with Co-Transplantation of Mesenchymal and Hematopoietic Stem Cells after Exposure to γ-Radiation. Bull Exp Biol Med. 2021 Dec;172(2):236-244. doi: 10.1007/s10517-021-05369-z. Epub 2021 Dec 02. PMID: 34855080.
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Bull Exp Biol Med. 2021 Dec;172(2):236-244. doi: 10.1007/s10517-021-05369-z. Epub 2021 Dec 02.

Experimental Study of the Efficacy of Sodium Deoxyribonucleate Used in Combination with Co-Transplantation of Mesenchymal and Hematopoietic Stem Cells after Exposure to γ-Radiation.

Bulletin of experimental biology and medicine

L N Pavlova, L P Zhavoronkov, V V Pavlov, V V Panfilova, O S Izmest'eva, O F Chibisova, V L Ivanov, P V Shegai, A D Kaprin

Affiliations

  1. A. F. Tsyb Medical Radiological Research Center, Affiliated Branch of the National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Obninsk, Russia. [email protected].
  2. A. F. Tsyb Medical Radiological Research Center, Affiliated Branch of the National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Obninsk, Russia.
  3. Clinical Hospital No. 8, Federal Medical-Biological Agency of Russia, Obninsk, Russia.
  4. National Medical Research Centre of Radiology, Ministry of Health of the Russian Federation, Obninsk, Russia.
  5. Peoples' Friendship University of Russia, Moscow, Russia.

PMID: 34855080 DOI: 10.1007/s10517-021-05369-z

Abstract

We studied the possibility of using sodium deoxyribonucleate (Derinat) for improving the efficiency of co-transplantation of mesenchymal (MSC) and hematopoietic stem cells (HSC) to female F1(CBA×C57BL/6) mice with bone marrow aplasia caused by exposure to γ-radiation. It was found that immunomodulator Derinat enhanced the effect of co-transplantation, in particular, triple post-irradiation administration of Derinat accelerated hematopoiesis recovery judging from the parameters of peripheral blood, total cellularity of the bone marrow and spleen, and animal survival. Single or double administration of Derinat prior to irradiation was ineffective. The optimal result was obtained when the following scheme was applied: MSC→HSC with an interval of 48 h starting during the first hours after irradiation and triple administration of Derinat (in 10-15 min, 3 and 7 days after irradiation) in a dose of 3 mg/mouse.

© 2021. Springer Science+Business Media, LLC, part of Springer Nature.

Keywords: co-transplantation; hemopoiesis; linear mice; sodium deoxyribonucleate; stem cells

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