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Pezoldt J, Wiechers C, Erhard F, et al. Single-cell transcriptional profiling of splenic fibroblasts reveals subset-specific innate immune signatures in homeostasis and during viral infection. Commun Biol. 2021;4(1):1355doi: 10.1038/s42003-021-02882-9.
Pezoldt, J., Wiechers, C., Erhard, F., Rand, U., Bulat, T., Beckstette, M., Brendolan, A., Huehn, J., Kalinke, U., Mueller, M., Strobl, B., Deplancke, B., Čičin-Šain, L., & Sitnik, K. M. (2021). Single-cell transcriptional profiling of splenic fibroblasts reveals subset-specific innate immune signatures in homeostasis and during viral infection. Communications biology, 4(1), 1355. https://doi.org/10.1038/s42003-021-02882-9
Pezoldt, Joern, et al. "Single-cell transcriptional profiling of splenic fibroblasts reveals subset-specific innate immune signatures in homeostasis and during viral infection." Communications biology vol. 4,1 (2021): 1355. doi: https://doi.org/10.1038/s42003-021-02882-9
Pezoldt J, Wiechers C, Erhard F, Rand U, Bulat T, Beckstette M, Brendolan A, Huehn J, Kalinke U, Mueller M, Strobl B, Deplancke B, Čičin-Šain L, Sitnik KM. Single-cell transcriptional profiling of splenic fibroblasts reveals subset-specific innate immune signatures in homeostasis and during viral infection. Commun Biol. 2021 Dec 02;4(1):1355. doi: 10.1038/s42003-021-02882-9. PMID: 34857864.
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Commun Biol. 2021 Dec 02;4(1):1355. doi: 10.1038/s42003-021-02882-9.

Single-cell transcriptional profiling of splenic fibroblasts reveals subset-specific innate immune signatures in homeostasis and during viral infection.

Communications biology

Joern Pezoldt, Carolin Wiechers, Florian Erhard, Ulfert Rand, Tanja Bulat, Michael Beckstette, Andrea Brendolan, Jochen Huehn, Ulrich Kalinke, Mathias Mueller, Birgit Strobl, Bart Deplancke, Luka Čičin-Šain, Katarzyna M Sitnik

Affiliations

  1. Laboratory of Systems Biology and Genetics, École Polytechnique Fédérale de Lausanne, 1015, Lausanne, CH, Switzerland.
  2. Department of Experimental Immunology, Helmholtz Centre for Infection Research, 38124, Braunschweig, Germany.
  3. Institute for Virology and Immunobiology, Julius-Maximilians-Universität Würzburg, 97070, Würzburg, Germany.
  4. Department of Viral Immunology, Helmholtz Centre for Infection Research, 38124, Braunschweig, Germany.
  5. Institute of Animal Breeding and Genetics, Department of Biomedical Science, University of Veterinary Medicine Vienna, 1210, Vienna, Austria.
  6. Department of Computational Biology for Individualized Medicine, Centre for Individualized Infection Medicine, 30625, Hannover, Germany.
  7. Unit of Lymphoid Organ Development, Division of Experimental Oncology, IRCCS San Raffaele Scientific Institute, Milan, Italy.
  8. Institute for Experimental Infection Research, TWINCORE, Centre for Experimental and Clinical Infection Research, a joint venture between the Helmholtz Centre for Infection Research, Braunschweig, and the Hannover Medical School, 30625, Hannover, Germany.
  9. Department of Viral Immunology, Helmholtz Centre for Infection Research, 38124, Braunschweig, Germany. [email protected].
  10. Department of Viral Immunology, Helmholtz Centre for Infection Research, 38124, Braunschweig, Germany. [email protected].

PMID: 34857864 DOI: 10.1038/s42003-021-02882-9

Abstract

Our understanding of the composition and functions of splenic stromal cells remains incomplete. Here, based on analysis of over 20,000 single cell transcriptomes of splenic fibroblasts, we characterized the phenotypic and functional heterogeneity of these cells in healthy state and during virus infection. We describe eleven transcriptionally distinct fibroblastic cell clusters, reassuring known subsets and revealing yet unascertained heterogeneity amongst fibroblasts occupying diverse splenic niches. We further identify striking differences in innate immune signatures of distinct stromal compartments in vivo. Compared to other fibroblasts and to endothelial cells, Ly6C

© 2021. The Author(s).

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