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Volf J, Rajova J, Babak V, et al. Detoxification, Hydrogen Sulphide Metabolism and Wound Healing Are the Main Functions That Differentiate Caecum Protein Expression from Ileum of Week-Old Chicken. Animals (Basel). 2021;11(11)doi: 10.3390/ani11113155.
Volf, J., Rajova, J., Babak, V., Seidlerova, Z., & Rychlik, I. (2021). Detoxification, Hydrogen Sulphide Metabolism and Wound Healing Are the Main Functions That Differentiate Caecum Protein Expression from Ileum of Week-Old Chicken. Animals : an open access journal from MDPI, 11(11), . https://doi.org/10.3390/ani11113155
Volf, Jiri, et al. "Detoxification, Hydrogen Sulphide Metabolism and Wound Healing Are the Main Functions That Differentiate Caecum Protein Expression from Ileum of Week-Old Chicken." Animals : an open access journal from MDPI vol. 11,11 (2021). doi: https://doi.org/10.3390/ani11113155
Volf J, Rajova J, Babak V, Seidlerova Z, Rychlik I. Detoxification, Hydrogen Sulphide Metabolism and Wound Healing Are the Main Functions That Differentiate Caecum Protein Expression from Ileum of Week-Old Chicken. Animals (Basel). 2021 Nov 04;11(11). doi: 10.3390/ani11113155. PMID: 34827887; PMCID: PMC8614574.
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Animals (Basel). 2021 Nov 04;11(11). doi: 10.3390/ani11113155.

Detoxification, Hydrogen Sulphide Metabolism and Wound Healing Are the Main Functions That Differentiate Caecum Protein Expression from Ileum of Week-Old Chicken.

Animals : an open access journal from MDPI

Jiri Volf, Jana Rajova, Vladimir Babak, Zuzana Seidlerova, Ivan Rychlik

Affiliations

  1. Department of Microbiology and Antimicrobial Resistance, Veterinary Research Institute, Hudcova 296/70, 621 00 Brno, Czech Republic.

PMID: 34827887 PMCID: PMC8614574 DOI: 10.3390/ani11113155

Abstract

Sections of chicken gut differ in many aspects, e.g., the passage of digesta (continuous vs. discontinuous), the concentration of oxygen, and the density of colonising microbiota. Using an unbiased LC-MS/MS protocol, we compared protein expression in 18 ileal and 57 caecal tissue samples that originated from 7-day old ISA brown chickens. We found that proteins specific to the ileum were either structural (e.g., 3 actin isoforms, villin, or myosin 1A), or those required for nutrient digestion (e.g., sucrose isomaltase, maltase-glucoamylase, peptidase D) and absorption (e.g., fatty acid-binding protein 2 and 6 or bile acid-CoA:amino acid N-acyltransferase). On the other hand, proteins characteristic of the caecum were involved in sensing and limiting the consequences of oxidative stress (e.g., thioredoxin, peroxiredoxin 6), cell adhesion, and motility associated with wound healing (e.g., fibronectin 1, desmoyokin). These mechanisms are coupled with the activation of mechanisms suppressing the inflammatory response (galectin 1). Rather prominent were also expressions of proteins linked to hydrogen sulphide metabolism in caecum represented by cystathionin beta synthase, selenium-binding protein 1, mercaptopyruvate sulphurtransferase, and thiosulphate sulphurtransferase. Higher mRNA expression of nuclear factor, erythroid 2-like 2, the main oxidative stress transcriptional factor in caecum, further supported our observations.

Keywords: caecum; chicken; hydrogen sulphide; ileum; stress response; wound healing

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