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Int J Mol Sci. 2021 Nov 05;22(21). doi: 10.3390/ijms222111979.

A Novel Method of Mouse RPE Explant Culture and Effective Introduction of Transgenes Using Adenoviral Transduction for .

International journal of molecular sciences

Peng Shang, Nadezda A Stepicheva, Haitao Liu, Olivia Chowdhury, Jonathan Franks, Ming Sun, Stacey Hose, Sayan Ghosh, Meysam Yazdankhah, Anastasia Strizhakova, Donna Beer Stolz, J Samuel Zigler, Debasish Sinha

Affiliations

  1. Department of Ophthalmology, Children's Hospital of University of Pittsburgh School of Medicine, One Children's Hospital Drive, 4401 Penn Avenue, Pittsburgh, PA 15224, USA.
  2. Department of Cell Biology and Center for Biologic Imaging, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.
  3. Wilmer Eye Institute, The Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.

PMID: 34769409 PMCID: PMC8584596 DOI: 10.3390/ijms222111979

Abstract

Degeneration of retinal pigment epithelium (RPE) is one of the most critical phenotypic changes of age-related macular degeneration (AMD), the leading cause of vision loss in the elderly. While cultured polarized RPE cells with original properties are valuable in

Keywords: RPE flatmount; adenoviral transduction; explant culture; retina pigment epithelium

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