Display options
Cite
Del Valle L. Introduction to Immunohistochemistry: From to Evolving Science to Timeless Art. Methods Mol Biol. 2022;2422:1-16doi: 10.1007/978-1-0716-1948-3_1.
Del Valle, L. (2022). Introduction to Immunohistochemistry: From to Evolving Science to Timeless Art. Methods in molecular biology (Clifton, N.J.), 24221-16. https://doi.org/10.1007/978-1-0716-1948-3_1
Del Valle, Luis. "Introduction to Immunohistochemistry: From to Evolving Science to Timeless Art." Methods in molecular biology (Clifton, N.J.) vol. 2422 (2022): 1-16. doi: https://doi.org/10.1007/978-1-0716-1948-3_1
Del Valle L. Introduction to Immunohistochemistry: From to Evolving Science to Timeless Art. Methods Mol Biol. 2022;2422:1-16. doi: 10.1007/978-1-0716-1948-3_1. PMID: 34859395.
Copy Download .nbib Format: NLM
Share it on

Methods Mol Biol. 2022;2422:1-16. doi: 10.1007/978-1-0716-1948-3_1.

Introduction to Immunohistochemistry: From to Evolving Science to Timeless Art.

Methods in molecular biology (Clifton, N.J.)

Luis Del Valle

Affiliations

  1. Department of Pathology and Medicine & Louisiana Cancer Research Center, Louisiana State University Health Sciences Center, New Orleans, LA, USA. [email protected].

PMID: 34859395 DOI: 10.1007/978-1-0716-1948-3_1

Abstract

Immunohistochemistry and all techniques that use antibodies and fluorescence are widespread, essential and irreplaceable tools used in both research laboratory settings and diagnostic pathology laboratories. The field was born approximately 80 years ago, with the idea that antibodies could be tagged with fluorescent substances and used to detect antigens in cells and microorganisms, and has vertiginously evolved since; these advances have come in all aspects of the methodology, tissue fixation, generation of antibodies, monoclonal antibodies, signal amplification, antigen retrieval, signal amplification, microscopy and have become increasingly sophisticated, from in situ hybridization, in situ proximity ligation assay, flow cytometry, comet assay, to multiplexing and green fluorescent protein reconstitution, yielding Nobel Prizes along the way and generating invaluable scientific and diagnostic advances as well as timeless beautiful images.

© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Keywords: Antibodies; Antibody structure; Antigen retrieval; Avidin-Biotin; Confocal microscopy; History; Immunofluorescence; Immunohistochemistry; Immunoperoxidase; In situ hybridization; Multiplexing; Quantum dots; Tyramide signal amplification

References

  1. Metchnikoff E (1887) Sur la lutte des cellules de l’organisme contre l’invasion des microbes. Ann Inst Pasteur 1:321 - PubMed
  2. Metchnikoff O (1921) The life of Elie Metchnikoff, 1845-1916. Houghton Mifflin Company, Boston, p 23ff - PubMed
  3. Ehrlich P (1901) Die Seitenkettentheorie und ihre Gegner. Münch Med Wschr 18:2123 - PubMed
  4. Valent P, Groner B, Schumacher U, Superti-Furga G, Busslinger M, Kralovics R, Zielinski C, Penninger JM, Kerjaschki D, Stingl G, Smolen JS, Valenta R, Lassmann H, Kovar H, Jäger U, Kornek G, Müller M, Sörgel F (2016) Paul Ehrlich (1854-1915) and his contributions to the foundation and birth of translational medicine. J Innate Immun 8(2):111–120 - PubMed
  5. The Nobel Prize in Physiology and Medicine 1908. nobelprize.org - PubMed
  6. Kaufmann SH (2008) Immunology’s foundation: the 100-year anniversary of the Nobel prize to Paul Ehrlich and Elie Metchnikoff. Nat Immunol 9:705–712 - PubMed
  7. Schmalstieg FC, Goldman AS (2008) Ilya Ilich Metchnikoff (1845-1915) and Paul Ehrlich (1854-1915): the centennial of the 1908 Nobel prize in physiology or medicine. J Med Biogr 16(2):96–103 - PubMed
  8. Ehrlich P (1891) Experimentelle Untersuchungen ubre Immunitat.II. Ueber Abrin. Dtsch Med Wochenschr 17:1218 - PubMed
  9. Janeway CA Jr, Travers P, Walport M, Shlomchik MJ (2001) The structure of a typical antibody molecule. In: 5th ed (ed) Immunobiology: the immune system in health and disease. Garland Science, New York - PubMed
  10. Marrack J (1934) Nature of antibodies. Nature 133:292–293 - PubMed
  11. Coons AH, Creech H, Jones R (1941) Immunological properties of an antibody containing a fluorescent group. Proc Soc Exp Biol Med 47:200–202 - PubMed
  12. Coons AH, Creech H, Jones R, Berliner E (1942) The demonstration of pneumococcal antigen in tissues by the use of fluorescent antibody. J Immunol 45:159–170 - PubMed
  13. Coons AH, Kaplan MH (1950) Localization of antigen in tissue cells II: improvements in a method for the detection of antigen by means of fluorescent antibody. J Exp Med 91(1):1–13 - PubMed
  14. Coons AH, Snyder JC, Sargent Cheever F, Murray ES (1950) Localization of antigen in tissue cells; antigens of rickettsiae and mumps virus. J Exp Med 91(1):31–38 - PubMed
  15. Coons AH (1960) Immunofluorescence. J Immunol 75:937–943 - PubMed
  16. Morikawa S, Coons AH (1976) Enumeration of specific antibody-forming cells of the mouse spleen after stimulation with protein antigens. Proc Soc Exp Biol Med 153(1):44–48 - PubMed
  17. Graham R Jr, Karnovsky MJ (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14(4):291–302 - PubMed
  18. Avrameas S, Lespinats G (1967) Enzymes coupled to proteins: their utilization for the detection of antigens and antibodies. C R Acad Hebd Seances Acad Sci D 265(16):1149–1152 - PubMed
  19. Avrameas S, Guilbert B (1971) Enzymo-immunological determination of proteins with the aid of immunoadsorbants and enzyme-labelled antigens. C R Acad Sci Hebd Seances Acad Sci D 273(25):2705–2707 - PubMed
  20. Nakane PK, Pierce GB (1967) Localization of tissue antigens on the ultrathin sections with peroxidase-labeled antibody method. J Histochem Cytochem 18(3):161–166 - PubMed
  21. Nakane P (1968) Simultaneous localization of multiple tissue antigens using the peroxidase-labeled antibody method: a study on pituitary glands of the rat. J Histochem Cytochem 16(9):557–560 - PubMed
  22. Mason T, Phifer RF, Spicer SS, Swallow RA, Dreskin RB (1969) An immunoglobulin-enzyme bridge method for localizing tissue antigens. J Histochem Cytochem 17(9):563–569 - PubMed
  23. Sternberger L, Hardy PH Jr, Cuculis JJ, Meyer HG (1970) The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–333 - PubMed
  24. Mason DY, Sammons R (1978) Alkaline phosphatase and peroxidase for double immunoenzymatic labelling of cellular constituents. J Clin Pathol 31(5):454–460 - PubMed
  25. Taylor CR, Burns J (1974) The demonstration of plasma cells and other immunoglobulin-containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase-labelled antibody. J Clin Pathol 27(1):14–20 - PubMed
  26. Hsu SM, Raine L, Fanger H (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29(4):577–580 - PubMed
  27. Hsu SM, Raine L (1981) Protein A, avidin, and biotin in immunohistochemistry. J Histochem Cytochem 29(11):1349–1353 - PubMed
  28. Bayer EA, Wilchek M, Skutelsky E (1976) Affinity cytochemistry: the localization of lectin and antibody receptors on erythrocytes via the avidin-biotin complex. FEBS Lett 68(2):240–244 - PubMed
  29. Green NM (1963) Avidin. 1. The use of (14-C)Biotin for kinetic studies and for assay. Biochem J 89(3):585–591 - PubMed
  30. Köhler G, Milstein C (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256(5517):495–497 - PubMed
  31. McMichael AJ, Pilch JR, Galfre G, Mason DY, Fabre JW, Milstein C (1979) A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody. Eur J Immunol 9(3):205–210 - PubMed
  32. Shi SR, Key ME, Kalra KL (1991) Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem 39:741–748 - PubMed
  33. Bobrow MN, Harris TD, Shaughnessy KJ, Litt GJ (1989) Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays. J Immunol Methods 125(1–2):279–285 - PubMed
  34. Adams JC (1992) Biotin amplification of biotin and horseradish peroxidase signals in histochemical stains. J Histochem Cytochem 40(10):1457–1463 - PubMed
  35. Hasui K, Murata F (2005) A new simplified catalyzed signal amplification system for minimizing non-specific staining in tissues with supersensitive immunohistochemistry. Arch Histol Cytol 68(1):1017 - PubMed
  36. Mohsen MG, Kool ET (2016) The discovery of the rolling circle amplification and rolling circle transcription. Acc Chem Res 49(11):2540–2550 - PubMed
  37. Sano T, Smith CL, Cantor CR (1992) Immuno-PCR: very sensitive detection by means of specific antibody-DNA conjugates. Science 258(5079):120–122 - PubMed
  38. Fredriksson S, Gullberg M, Jarvius J, Olsson C, Pietras K, Gústafsdóttir SM, Ostman A, Landegren U (2002) Protein detection using proximity-dependent DNA ligation assays. Nat Biotechnol 20(5):473–477 - PubMed
  39. US Patent US3013467A, filed in 1957 and granted in 1961 - PubMed
  40. Davidovits P, Egger MD (1969) Scanning laser microscope. Nature 223(5208):831 - PubMed
  41. Ness JM, Akhtar RS, Latham CB, Roth KA (2003) Combined tyramide signal amplification and quantum dots for sensitive and photostable immunofluorescence detection. J Histochem Cytochem 51(8):981–987 - PubMed
  42. Akhtar RS, Latham CB, Siniscalco D, Fuccio C, Roth KA (2007) Immunohistochemical detection of quantum dots. Methods Mol Biol 374:11–28 - PubMed
  43. Chen K, Xiang L, Xu K (2021) Excitation spectral microscopy for highly multiplexed fluorescence imaging and quantitative biosensing. Light Sci Appl 10:97 - PubMed
  44. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC (1994) Green fluorescent protein as a marker for gene expression. Science 263(5148):802–805 - PubMed
  45. Pierce AJ, Jasin M (2005) Measuring recombination proficiency in mouse embryonic stem cells. Methods Mol Biol 291:373–384 - PubMed
  46. Nakanishi K, Cavallo F, Brunet E, Jasin M (2011) Homologous recombination assay for interstrand cross-link repair. Methods Mol Biol 745:283–291 - PubMed

Publication Types