Display options
Cite
Alfeo MJ, Pagotto A, Barbieri G, et al. Staphylococcus aureus iron-regulated surface determinant B (IsdB) protein interacts with von Willebrand factor and promotes adherence to endothelial cells. Sci Rep. 2021;11(1):22799doi: 10.1038/s41598-021-02065-w.
Alfeo, M. J., Pagotto, A., Barbieri, G., Foster, T. J., Vanhoorelbeke, K., De Filippis, V., Speziale, P., & Pietrocola, G. (2021). Staphylococcus aureus iron-regulated surface determinant B (IsdB) protein interacts with von Willebrand factor and promotes adherence to endothelial cells. Scientific reports, 11(1), 22799. https://doi.org/10.1038/s41598-021-02065-w
Alfeo, Mariangela J, et al. "Staphylococcus aureus iron-regulated surface determinant B (IsdB) protein interacts with von Willebrand factor and promotes adherence to endothelial cells." Scientific reports vol. 11,1 (2021): 22799. doi: https://doi.org/10.1038/s41598-021-02065-w
Alfeo MJ, Pagotto A, Barbieri G, Foster TJ, Vanhoorelbeke K, De Filippis V, Speziale P, Pietrocola G. Staphylococcus aureus iron-regulated surface determinant B (IsdB) protein interacts with von Willebrand factor and promotes adherence to endothelial cells. Sci Rep. 2021 Nov 23;11(1):22799. doi: 10.1038/s41598-021-02065-w. PMID: 34815454; PMCID: PMC8611056.
Copy Download .nbib Format: NLM
Share it on

Sci Rep. 2021 Nov 23;11(1):22799. doi: 10.1038/s41598-021-02065-w.

Staphylococcus aureus iron-regulated surface determinant B (IsdB) protein interacts with von Willebrand factor and promotes adherence to endothelial cells.

Scientific reports

Mariangela J Alfeo, Anna Pagotto, Giulia Barbieri, Timothy J Foster, Karen Vanhoorelbeke, Vincenzo De Filippis, Pietro Speziale, Giampiero Pietrocola

Affiliations

  1. Department of Molecular Medicine, Biochemistry Unit, University of Pavia, Viale Taramelli 3/b, 27100, Pavia, Italy.
  2. Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Via Marzolo 5, 35131, Padua, Italy.
  3. Department of Biology and Biotechnology "Lazzaro Spallanzani", University of Pavia, Via Ferrata 9, 27100, Pavia, Italy.
  4. Microbiology Department, Trinity College Dublin, Dublin, Ireland.
  5. Laboratory for Thrombosis Research, KU Leuven Campus Kulak Kortrijk, Kortrijk, Belgium.
  6. Department of Molecular Medicine, Biochemistry Unit, University of Pavia, Viale Taramelli 3/b, 27100, Pavia, Italy. [email protected].

PMID: 34815454 PMCID: PMC8611056 DOI: 10.1038/s41598-021-02065-w

Abstract

Staphylococcus aureus is the cause of a spectrum of diseases in humans and animals. The molecular basis of this pathogenicity lies in the expression of a variety of virulence factors, including proteins that mediate adherence to the host plasma and extracellular matrix proteins. In this study, we discovered that the iron-regulated surface determinant B (IsdB) protein, besides being involved in iron transport and vitronectin binding, interacts with von Willebrand Factor (vWF). IsdB-expressing bacteria bound to both soluble and immobilized vWF. The binding of recombinant IsdB to vWF was blocked by heparin and reduced at high ionic strength. Furthermore, treatment with ristocetin, an allosteric agent that promotes the exposure of the A1 domain of vWF, potentiates the binding of IsdB to vWF. Both near-iron transporter motifs NEAT1 and NEAT2 of IsdB individually bound recombinant A1 domain with K

© 2021. The Author(s).

References

  1. Mol Microbiol. 2007 Feb;63(3):711-23 - PubMed
  2. Sci Rep. 2019 Dec 9;9(1):18629 - PubMed
  3. Anal Biochem. 1995 May 1;227(1):176-85 - PubMed
  4. J Biol Chem. 1998 Mar 20;273(12):6821-9 - PubMed
  5. J Biol Chem. 2018 Jan 5;293(1):177-190 - PubMed
  6. J Biol Chem. 2013 Sep 27;288(39):28283-92 - PubMed
  7. mBio. 2019 Apr 30;10(2): - PubMed
  8. Mol Microbiol. 1998 Oct;30(2):245-57 - PubMed
  9. Mol Microbiol. 2006 Jan;59(1):212-30 - PubMed
  10. mBio. 2021 Aug 31;12(4):e0116721 - PubMed
  11. Res Pract Thromb Haemost. 2021 Jun 05;5(5):e12534 - PubMed
  12. Curr Opin Biotechnol. 1997 Feb 1;8(1):50-7 - PubMed
  13. Biometals. 2006 Apr;19(2):193-203 - PubMed
  14. Microbiology (Reading). 2010 Mar;156(Pt 3):920-928 - PubMed
  15. Microbiology (Reading). 1998 Dec;144 ( Pt 12):3387-3395 - PubMed
  16. Blood. 2014 Sep 4;124(10):1669-76 - PubMed
  17. Microbiol Spectr. 2019 Jul;7(4): - PubMed
  18. Mol Microbiol. 2004 Mar;51(5):1509-19 - PubMed
  19. J Biol Chem. 2014 Dec 26;289(52):35979-86 - PubMed
  20. Microvasc Res. 2011 Jul;82(1):66-72 - PubMed
  21. PLoS Pathog. 2012 Dec;8(12):e1003092 - PubMed
  22. Eur J Clin Invest. 2006 Aug;36(8):536-43 - PubMed
  23. J Infect Dis. 2016 Apr 1;213(7):1148-56 - PubMed
  24. Mol Cell Proteomics. 2012 Nov;11(11):1123-39 - PubMed
  25. Nat Rev Microbiol. 2014 Jan;12(1):49-62 - PubMed
  26. FEMS Microbiol Lett. 2004 May 1;234(1):155-61 - PubMed
  27. Appl Environ Microbiol. 1996 Oct;62(10):3662-7 - PubMed
  28. Front Microbiol. 2020 Aug 26;11:2054 - PubMed
  29. Blood. 2009 Feb 12;113(7):1589-97 - PubMed
  30. J Thromb Haemost. 2003 Jul;1(7):1335-42 - PubMed
  31. J Biol Chem. 2000 May 5;275(18):13863-71 - PubMed
  32. Eur J Biochem. 1997 Jul 1;247(1):416-24 - PubMed
  33. Blood. 2015 Apr 2;125(14):2297-304 - PubMed
  34. J Biol Chem. 1987 Oct 5;262(28):13835-41 - PubMed
  35. Cell Host Microbe. 2007 May 17;1(3):199-212 - PubMed
  36. Microbiology (Reading). 2009 Mar;155(Pt 3):667-679 - PubMed
  37. Nano Lett. 2020 Dec 9;20(12):8919-8925 - PubMed
  38. J Biol Chem. 2020 Jul 17;295(29):10008-10022 - PubMed
  39. J Biol Chem. 1998 Apr 24;273(17):10396-401 - PubMed
  40. Circulation. 2013 Jul 2;128(1):50-9 - PubMed
  41. Proc Natl Acad Sci U S A. 2009 May 12;106(19):7786-91 - PubMed
  42. Cell Mol Life Sci. 2015 Jan;72(2):307-26 - PubMed
  43. Clin Microbiol Rev. 2020 Dec 23;34(2): - PubMed
  44. Commun Biol. 2021 Apr 12;4(1):453 - PubMed
  45. Vaccines (Basel). 2021 May 04;9(5): - PubMed
  46. Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10037-41 - PubMed
  47. Nat Rev Microbiol. 2014 Aug;12(8):585-91 - PubMed
  48. Biochemistry. 2016 Jul 19;55(28):3984-94 - PubMed
  49. Eur Biophys J. 2010 Nov;39(12):1597-603 - PubMed
  50. FEBS J. 2011 Jul;278(13):2359-71 - PubMed
  51. J Thromb Haemost. 2013 Jun;11(6):1093-102 - PubMed
  52. J Biol Chem. 1987 Feb 5;262(4):1734-9 - PubMed
  53. Annu Rev Microbiol. 2011;65:129-47 - PubMed
  54. Eur Heart J. 2015 Nov 21;36(44):3075-3128 - PubMed
  55. Hum Vaccin Immunother. 2014;10(12):3513-6 - PubMed
  56. FEBS J. 2006 Nov;273(21):4831-41 - PubMed
  57. Nat Struct Mol Biol. 2005 Feb;12(2):152-9 - PubMed
  58. J Innate Immun. 2012;4(2):141-8 - PubMed
  59. Arterioscler Thromb. 1993 Mar;13(3):398-406 - PubMed
  60. Biochemistry. 1981 Apr 28;20(9):2361-70 - PubMed
  61. Blood. 2000 Sep 15;96(6):2149-56 - PubMed
  62. ACS Nano. 2017 Feb 28;11(2):2160-2170 - PubMed
  63. J Bacteriol. 2002 Oct;184(19):5457-67 - PubMed
  64. Microbiology (Reading). 2002 Jul;148(Pt 7):2037-2044 - PubMed
  65. Cell Microbiol. 2013 Jun;15(6):1026-41 - PubMed
  66. PLoS One. 2011 Apr 15;6(4):e18748 - PubMed

Publication Types