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Ahmed MFE, Alssahen M, Lämmler C, et al. Identification of Trueperella bernardiae isolated from peking ducks (Anas platyrhynchos domesticus) by phenotypical and genotypical investigations and by a newly developed loop-mediated isothermal amplification (LAMP) assay. Folia Microbiol (Praha). 2021;doi: 10.1007/s12223-021-00927-4.
Ahmed, M. F. E., Alssahen, M., Lämmler, C., Köhler, B., Metzner, M., Plötz, M., & Abdulmawjood, A. (2021). Identification of Trueperella bernardiae isolated from peking ducks (Anas platyrhynchos domesticus) by phenotypical and genotypical investigations and by a newly developed loop-mediated isothermal amplification (LAMP) assay. Folia microbiologica, . https://doi.org/10.1007/s12223-021-00927-4
Ahmed, Marwa F E, et al. "Identification of Trueperella bernardiae isolated from peking ducks (Anas platyrhynchos domesticus) by phenotypical and genotypical investigations and by a newly developed loop-mediated isothermal amplification (LAMP) assay." Folia microbiologica vol. (2021). doi: https://doi.org/10.1007/s12223-021-00927-4
Ahmed MFE, Alssahen M, Lämmler C, Köhler B, Metzner M, Plötz M, Abdulmawjood A. Identification of Trueperella bernardiae isolated from peking ducks (Anas platyrhynchos domesticus) by phenotypical and genotypical investigations and by a newly developed loop-mediated isothermal amplification (LAMP) assay. Folia Microbiol (Praha). 2021 Nov 15; doi: 10.1007/s12223-021-00927-4. Epub 2021 Nov 15. PMID: 34780047.
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Folia Microbiol (Praha). 2021 Nov 15; doi: 10.1007/s12223-021-00927-4. Epub 2021 Nov 15.

Identification of Trueperella bernardiae isolated from peking ducks (Anas platyrhynchos domesticus) by phenotypical and genotypical investigations and by a newly developed loop-mediated isothermal amplification (LAMP) assay.

Folia microbiologica

Marwa F E Ahmed, Mazen Alssahen, Christoph Lämmler, Bernd Köhler, Martin Metzner, Madeleine Plötz, Amir Abdulmawjood

Affiliations

  1. Hygiene and Zoonoses Department, Faculty of Veterinary Medicine, Mansoura University, Elgomhoria Street 60, Mansoura, 35516, Egypt.
  2. Institute for Animal Nutrition, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173, Hannover, Germany.
  3. Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Gießen, Frankfurterstraße 85-91, 35392, Gießen, Germany.
  4. Ripac-Labor GmbH, Am Mühlenberg 11, 14476, Potsdam, Germany.
  5. Institute of Food Quality and Food Safety, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173, Hannover, Germany.
  6. Institute of Food Quality and Food Safety, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173, Hannover, Germany. [email protected].

PMID: 34780047 DOI: 10.1007/s12223-021-00927-4

Abstract

Trueperella (T.) bernardiae is a well-known bacterial pathogen in infections of humans, rarely in animals. In the present study, five T. bernardiae isolates, isolated from five Peking ducks of four different farms, were identified by phenotypic properties, by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, and genotypically by sequencing the 16S ribosomal RNA (rRNA) gene, the superoxide dismutase A encoding gene sodA, and the glyceraldehyde-3-phosphate dehydrogenase encoding gene gap. In addition, the T. bernardiae isolates could be identified with a newly developed loop-mediated isothermal amplification (LAMP) assay based on the gyrase encoding housekeeping gene gyrA. All these tests clearly identified the T. bernardiae isolates to the species level. However, the detection of the specific gene gyrA with the newly designed LAMP assay appeared with a high sensitivity and specificity, and could help to identify this bacterial species in human and animal infections in future. The importance of the T. bernardiae isolates for the clinical condition of the ducks and for the problems at farm level remains unclear.

© 2021. The Author(s).

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