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Nagy I, Szabó M, Hegyi A, et al. Salmonella Genomic Island 1 requires a self-encoded small RNA for mobilization. Mol Microbiol. 2021;doi: 10.1111/mmi.14846.
Nagy, I., Szabó, M., Hegyi, A., & Kiss, J. (2021). Salmonella Genomic Island 1 requires a self-encoded small RNA for mobilization. Molecular microbiology, . https://doi.org/10.1111/mmi.14846
Nagy, István, et al. "Salmonella Genomic Island 1 requires a self-encoded small RNA for mobilization." Molecular microbiology vol. (2021). doi: https://doi.org/10.1111/mmi.14846
Nagy I, Szabó M, Hegyi A, Kiss J. Salmonella Genomic Island 1 requires a self-encoded small RNA for mobilization. Mol Microbiol. 2021 Nov 16; doi: 10.1111/mmi.14846. Epub 2021 Nov 16. PMID: 34784078.
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Mol Microbiol. 2021 Nov 16; doi: 10.1111/mmi.14846. Epub 2021 Nov 16.

Salmonella Genomic Island 1 requires a self-encoded small RNA for mobilization.

Molecular microbiology

István Nagy, Mónika Szabó, Anna Hegyi, János Kiss

Affiliations

  1. Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, Gödöll?, Hungary.

PMID: 34784078 DOI: 10.1111/mmi.14846

Abstract

The SGI1-family elements that are specifically mobilized by the IncA- and IncC-family plasmids are important vehicles of antibiotic resistance among enteric bacteria. Although SGI1 exploits many plasmid-derived conjugation and regulatory functions, the basic mobilization module of the island is unrelated to that of IncC plasmids. This module contains the oriT and encodes the mobilization proteins MpsA and MpsB, which belong to the tyrosine recombinases and not to relaxases. Here we report an additional, essential transfer factor of SGI1. This is a small RNA deriving from the 3'-end of a primary RNA that can also serve as mRNA of ORF S022. The functional domain of this sRNA named sgm-sRNA is encoded between the mpsA gene and the oriT of SGI1. Terminator-like sequence near the promoter of the primary transcript possibly has a regulatory function in controlling the amount of full-length primary RNA, which is converted to the active sgm-sRNA through consecutive maturation steps influenced by the 5'-end of the primary RNA. The mobilization module of SGI1 seems unique due to its atypical relaxase and the newly identified sgm-sRNA, which is required for the horizontal transfer of the island but appears to act differently from classical regulatory sRNAs.

© 2021 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd.

Keywords: IncC plasmids; SGI1; conjugation; horizontal gene transfer; multiresistance; small RNA

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