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Sharma K, Bullock AJ, Giblin V, et al. Identification of a fibrin concentration that promotes skin cell outgrowth from skin explants onto a synthetic dermal substitute. JPRAS Open. 2020;25:8-17doi: 10.1016/j.jpra.2019.12.001.
Sharma, K., Bullock, A. J., Giblin, V., & MacNeil, S. (2020). Identification of a fibrin concentration that promotes skin cell outgrowth from skin explants onto a synthetic dermal substitute. JPRAS open, 258-17. https://doi.org/10.1016/j.jpra.2019.12.001
Sharma, Kavita, et al. "Identification of a fibrin concentration that promotes skin cell outgrowth from skin explants onto a synthetic dermal substitute." JPRAS open vol. 25 (2020): 8-17. doi: https://doi.org/10.1016/j.jpra.2019.12.001
Sharma K, Bullock AJ, Giblin V, MacNeil S. Identification of a fibrin concentration that promotes skin cell outgrowth from skin explants onto a synthetic dermal substitute. JPRAS Open. 2020 Apr 21;25:8-17. doi: 10.1016/j.jpra.2019.12.001. eCollection 2020 Sep. PMID: 32490127; PMCID: PMC7260611.
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JPRAS Open. 2020 Apr 21;25:8-17. doi: 10.1016/j.jpra.2019.12.001. eCollection 2020 Sep.

Identification of a fibrin concentration that promotes skin cell outgrowth from skin explants onto a synthetic dermal substitute.

JPRAS open

Kavita Sharma, Anthony J Bullock, Victoria Giblin, Sheila MacNeil

Affiliations

  1. Kroto Research Institute, Department of Materials Science and Engineering, University of Sheffield, North Campus, Broad Lane, Sheffield, S1 7HQ UK.

PMID: 32490127 PMCID: PMC7260611 DOI: 10.1016/j.jpra.2019.12.001

Abstract

BACKGROUND: Our overall objective is to develop a single-stage in-theatre skin replacement by combining small explants of skin with a synthetic biodegradable dermal scaffold. The aim of the current study is to determine the concentration of fibrin constituents and their handling properties for both adhering skin explants to the scaffold and encouraging cellular outgrowth to achieve reepithelialization.

METHODS: Small skin explants were combined with several concentrations of thrombin (2.5,4.5,and 6.5 I.U) and fibrinogen (18.75,67, and 86.5 mg/ml), cultured in Green's media for 14 days and cellular outgrowth was measured using Rose Bengal staining. They were also cultured on electrospun scaffolds for 14 and 21 days. Hematoxylin and eosin (H&E) staining was undertaken to visualize the interface between skin explants and scaffolds and metabolic activity and collagen production were assessed.

RESULTS: A thrombin/fibrinogen combination of 2.5 I. U/ml /18.75 mg/ml showed significantly greater cell viability as assessed by Rose Bengal stained areas at days 7 and 14. This was also seen in DAPI images and H&E stains skin explant/scaffold constructs. Fibrin with a concentration of thrombin 2.5 I.U./ml took 5-6 min to set, which is convenient for distributing skin explants on the scaffold.

CONCLUSION: The study identified concentrations of thrombin (2.5 I.U/ml) and fibrinogen (18.75 mg/ml), which were easy to handle and aided the retention of skin explants and permitted cell outgrowth from explants.

© 2020 The Author(s).

Keywords: Dermal substitute; Fibrin; Meek grafting; Tissue-engineered skin

Conflict of interest statement

None.

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