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Loyola-Leyva A, Alcántara-Quintana LE, Terán-Figueroa Y, et al. In vitro effect of high glucose concentrations on erythrocyte morphology assessed by scanning electron microscopy. Micron. 2021;103179doi: 10.1016/j.micron.2021.103179.
Loyola-Leyva, A., Alcántara-Quintana, L. E., Terán-Figueroa, Y., & González, F. J. (2021). In vitro effect of high glucose concentrations on erythrocyte morphology assessed by scanning electron microscopy. Micron (Oxford, England : 1993), 103179. https://doi.org/10.1016/j.micron.2021.103179
Loyola-Leyva, Alejandra, et al. "In vitro effect of high glucose concentrations on erythrocyte morphology assessed by scanning electron microscopy." Micron (Oxford, England : 1993) vol. (2021): 103179. doi: https://doi.org/10.1016/j.micron.2021.103179
Loyola-Leyva A, Alcántara-Quintana LE, Terán-Figueroa Y, González FJ. In vitro effect of high glucose concentrations on erythrocyte morphology assessed by scanning electron microscopy. Micron. 2021 Nov 09;103179. doi: 10.1016/j.micron.2021.103179. Epub 2021 Nov 09. PMID: 34895961.
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Micron. 2021 Nov 09;103179. doi: 10.1016/j.micron.2021.103179. Epub 2021 Nov 09.

In vitro effect of high glucose concentrations on erythrocyte morphology assessed by scanning electron microscopy.

Micron (Oxford, England : 1993)

Alejandra Loyola-Leyva, Luz E Alcántara-Quintana, Yolanda Terán-Figueroa, Francisco Javier González

Affiliations

  1. Coordination for Innovation and Application of Science and Technology (Coordinación para la Innovación y Aplicación de la Ciencia y Tecnología, CIACyT), Avenida Sierra Leona 550, Lomas 2ª sección, 78210, San Luis Potosí, S.L.P, Mexico. Electronic address: [email protected].
  2. Coordination for Innovation and Application of Science and Technology (Coordinación para la Innovación y Aplicación de la Ciencia y Tecnología, CIACyT), Avenida Sierra Leona 550, Lomas 2ª sección, 78210, San Luis Potosí, S.L.P, Mexico. Electronic address: [email protected].
  3. Faculty of Nursing and Nutrition, Autonomous University of San Luis Potosí (Universidad Autónoma de San Luis Potosí), Lateral Av. Salvador Nava, Lomas, 78290, San Luis Potosí, S.L.P, Mexico. Electronic address: [email protected].
  4. Coordination for Innovation and Application of Science and Technology (Coordinación para la Innovación y Aplicación de la Ciencia y Tecnología, CIACyT), Avenida Sierra Leona 550, Lomas 2ª sección, 78210, San Luis Potosí, S.L.P, Mexico. Electronic address: [email protected].

PMID: 34895961 DOI: 10.1016/j.micron.2021.103179

Abstract

AIMS: To evaluate the in vitro effect of different glucose concentrations and the time series in erythrocyte cell viability and morphology parameters.

METHODS: Different glucose concentrations were assessed, from a normal physiological glucose concentration (5 mM) to a diabetes scenario (100 mM). Cell viability was obtained by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay. The erythrocyte morphology (diameter, height, and axial ratio) was assessed by scanning electron microscopy (SEM).

RESULTS: A significant decrease in cell viability was observed in erythrocytes with higher glucose concentration (100 mM). There was also a significant increase in the cells' diameter and height exposed to 100 mM, vs. those cultured with 5, 20, and 45 mM glucose, but only at 24 and 48 h. When comparing the same glucose concentration by a time series, it was observed that erythrocytes from the culture with 45 and 100 mM of glucose had a significant continuous decrease (approximately -0.40 μm) in diameter and height (24, 48, and 72 h), but there was no difference in the axial ratio observed.

CONCLUSION: Continuous exposure to high glucose concentrations in diabetes models produced less cell viability and changes in erythrocyte morphology (larger and scrambled cells), which in vivo might impact in microvascular complications.

Copyright © 2021 Elsevier Ltd. All rights reserved.

Keywords: Cell viability; Diabetes; Erythrocytes; Glucose; Morphology; Scanning electron microscopy

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