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J Biotechnol. 2021 Dec 08; doi: 10.1016/j.jbiotec.2021.12.002. Epub 2021 Dec 08.

Green Biorefinery: Microalgae-Bacteria Microbiome on Tolerance Investigations in Plants.

Journal of biotechnology

Dong Bui-Xuan, Doris Ying Ying Tang, Kit Wayne Chew, Thi Dong Phuong Nguyen, Han Le Ho, Thi Ngoc Thu Tran, Toan Nguyen-Sy, Thi Ha Thuong Dinh, Phuc Son Nguyen, Thi My Huong Dinh, Thanh Thuy Nguyen, Pau Loke Show

Affiliations

  1. The University of Danang, University of Science and Technology, 54 Nguyen Luong Bang St., Danang, 550 000, Vietnam.
  2. Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia.
  3. School of Energy and Chemical Engineering, Xiamen University Malaysia, Jalan Sunsuria, Bandar Sunsuria, 43900 Sepang, Selangor Darul Ehsan, Malaysia; College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian, China. Electronic address: [email protected].
  4. The University of Danang, University of Technology and Education, 48 Cao Thang St., Danang, 550 000, Vietnam. Electronic address: [email protected].
  5. Biological Resource Center/Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, Jeonbuk 56212, Republic of Korea; University of Science and Technology (UST), Daejeon 34113, Republic of Korea.
  6. The University of Danang, University of Technology and Education, 48 Cao Thang St., Danang, 550 000, Vietnam.
  7. The University of Danang, University of Science and Education, 459 Ton Duc Thang St., Danang, 550 000, Vietnam.
  8. National Institute of Hygiene and Epidemiology, 01 Yecxanh St, Hanoi, Viet Nam.

PMID: 34896159 DOI: 10.1016/j.jbiotec.2021.12.002

Abstract

Co-culture of microalgae and microorganisms, supported with the resulting synergistic effects, can be used for wastewater treatment, biomass production, agricultural applications and etc. Therefore, this study aimed to explore the role of Bacillus subtilis (B. subtilis) in tolerance against the harsh environment of seafood wastewater, at which these microalgal-bacterial flocs were formed by microalgae cultivation. In this present study, B. subtilis isolated from the cultivation medium of Chlorella vulgaris and exposed to different salinity (0.1 to 4% w/v sodium chloride) and various pH range to determine the tolerant ability and biofilm formation. Interestingly, this bacteria strain that isolated from microalgae cultivation medium showed the intense viability in the salt concentration exceeding up to 4% (w/v) NaCl but demonstrated the decrease in cell division as environmental culture undergoing over pH 10. Cell viability was recorded higher than 71% and 92% for B. subtilis inoculum in media with salt concentration greater than 20 gL

Copyright © 2021. Published by Elsevier B.V.

Keywords: Bacillus sp.; Biofilm; Microalgae; Salinity tolerance; pH tolerance

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