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Suzuki K, Fukasawa J, Miura M, et al. Influence of Culture Period on Osteoblast Differentiation of Tissue-Engineered Bone Constructed by Apatite-Fiber Scaffolds Using Radial-Flow Bioreactor. Int J Mol Sci. 2021;22(23)doi: 10.3390/ijms222313080.
Suzuki, K., Fukasawa, J., Miura, M., Lim, P. N., Honda, M., Matsuura, T., & Aizawa, M. (2021). Influence of Culture Period on Osteoblast Differentiation of Tissue-Engineered Bone Constructed by Apatite-Fiber Scaffolds Using Radial-Flow Bioreactor. International journal of molecular sciences, 22(23), . https://doi.org/10.3390/ijms222313080
Suzuki, Kitaru, et al. "Influence of Culture Period on Osteoblast Differentiation of Tissue-Engineered Bone Constructed by Apatite-Fiber Scaffolds Using Radial-Flow Bioreactor." International journal of molecular sciences vol. 22,23 (2021). doi: https://doi.org/10.3390/ijms222313080
Suzuki K, Fukasawa J, Miura M, Lim PN, Honda M, Matsuura T, Aizawa M. Influence of Culture Period on Osteoblast Differentiation of Tissue-Engineered Bone Constructed by Apatite-Fiber Scaffolds Using Radial-Flow Bioreactor. Int J Mol Sci. 2021 Dec 03;22(23). doi: 10.3390/ijms222313080. PMID: 34884885; PMCID: PMC8657963.
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Int J Mol Sci. 2021 Dec 03;22(23). doi: 10.3390/ijms222313080.

Influence of Culture Period on Osteoblast Differentiation of Tissue-Engineered Bone Constructed by Apatite-Fiber Scaffolds Using Radial-Flow Bioreactor.

International journal of molecular sciences

Kitaru Suzuki, Jun Fukasawa, Maiko Miura, Poon Nian Lim, Michiyo Honda, Tomokazu Matsuura, Mamoru Aizawa

Affiliations

  1. Department of Applied Chemistry, School of Science and Technology, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki 214-8571, Kanagawa, Japan.
  2. International Institute for Materials with Life Functions, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki 214-8571, Kanagawa, Japan.
  3. Department of Laboratory Medicine, The Jikei University School of Medicine, 3-25-8 Nishi-Shinbashi, Minato-ku, Tokyo 105-8461, Japan.

PMID: 34884885 PMCID: PMC8657963 DOI: 10.3390/ijms222313080

Abstract

With the limitation of autografts, the development of alternative treatments for bone diseases to alleviate autograft-related complications is highly demanded. In this study, a tissue-engineered bone was formed by culturing rat bone marrow cells (RBMCs) onto porous apatite-fiber scaffolds (AFSs) with three-dimensional (3D) interconnected pores using a radial-flow bioreactor (RFB). Using the optimized flow rate, the effect of different culturing periods on the development of tissue-engineered bone was investigated. The 3D cell culture using RFB was performed for 0, 1 or 2 weeks in a standard medium followed by 0, 1 or 2 weeks in a differentiation medium. Osteoblast differentiation in the tissue-engineered bone was examined by alkaline phosphatase (ALP) and osteocalcin (OC) assays. Furthermore, the tissue-engineered bone was histologically examined by hematoxylin and eosin and alizarin red S stains. We found that the ALP activity and OC content of calcified cells tended to increase with the culture period, and the differentiation of tissue-engineered bone could be controlled by varying the culture period. In addition, the employment of RFB and AFSs provided a favorable 3D environment for cell growth and differentiation. Overall, these results provide valuable insights into the design of tissue-engineered bone for clinical applications.

Keywords: apatite-fiber scaffold; bioreactor; calcium phosphate; hydroxyapatite; rat bone marrow stem cells; tissue-engineered bone

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