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Qi Y, Shi L, Duan G, et al. Taurochenodeoxycholic Acid Increases cAMP Content via Specially Interacting with Bile Acid Receptor TGR5. Molecules. 2021;26(23)doi: 10.3390/molecules26237066.
Qi, Y., Shi, L., Duan, G., Ma, Y., & Li, P. (2021). Taurochenodeoxycholic Acid Increases cAMP Content via Specially Interacting with Bile Acid Receptor TGR5. Molecules (Basel, Switzerland), 26(23), . https://doi.org/10.3390/molecules26237066
Qi, Youchao, et al. "Taurochenodeoxycholic Acid Increases cAMP Content via Specially Interacting with Bile Acid Receptor TGR5." Molecules (Basel, Switzerland) vol. 26,23 (2021). doi: https://doi.org/10.3390/molecules26237066
Qi Y, Shi L, Duan G, Ma Y, Li P. Taurochenodeoxycholic Acid Increases cAMP Content via Specially Interacting with Bile Acid Receptor TGR5. Molecules. 2021 Nov 23;26(23). doi: 10.3390/molecules26237066. PMID: 34885648; PMCID: PMC8659238.
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Molecules. 2021 Nov 23;26(23). doi: 10.3390/molecules26237066.

Taurochenodeoxycholic Acid Increases cAMP Content via Specially Interacting with Bile Acid Receptor TGR5.

Molecules (Basel, Switzerland)

Youchao Qi, Linkai Shi, Guozhen Duan, Yonggui Ma, Peifeng Li

Affiliations

  1. Department of Basic Veterinary Medicine, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010010, China.
  2. Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease, Ministry of Agriculture, Hohhot 010010, China.
  3. Department of Veterinary Medicine, College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China.
  4. Agricultural and Pastoral Bureau of Togtoh County, Togtoh 010200, China.
  5. Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, China.
  6. Key Laboratory of Medicinal Animal and Plant Resources of Qinghai Tibetan Plateau, Qinghai Normal University, Xining 810008, China.
  7. Academy of Plateau Science and Sustainability, Qinghai Normal University, Xining 810008, China.

PMID: 34885648 PMCID: PMC8659238 DOI: 10.3390/molecules26237066

Abstract

Taurochenodeoxycholic acid (TCDCA) is one of the main components of bile acids (BAs). TCDCA has been reported as a signaling molecule, exerting anti-inflammatory and immunomodulatory functions. However, it is not well known whether those effects are mediated by TGR5. This study aimed to elucidate the interaction between TCDCA and TGR5. To achieve this aim, first, the TGR5 eukaryotic vector was constructed. The expression level of TGR5 in 293T cells was determined by immunofluorescence, real-time quantitative PCR (RT-PCR, qPCR), and Western blot. The luciferase assay, fluorescence microscopy, and enzyme-linked immunosorbent assay (ELISA) were recruited to check the interaction of TCDCA with TGR5. TCDCA treatment in 293T cells resulted in TGR5 internalization coupled with a significant increase in cAMP luciferase expression. Our results demonstrated that TCDCA was able to bind to the TGR5 receptor and activate it. These results provide an excellent potential therapeutic target for TCDCA research. Moreover, these findings also provide theoretical evidence for further TCDCA research.

Keywords: 239T cells; TGR5; cyclic adenosine monophosphate; interaction; taurochenodeoxycholic acid

References

  1. Obesity (Silver Spring). 2009 Sep;17(9):1671-7 - PubMed
  2. Eur J Pharmacol. 2013 Apr 15;706(1-3):36-40 - PubMed
  3. Int J Cancer. 2016 Oct 15;139(8):1764-75 - PubMed
  4. Am J Physiol Gastrointest Liver Physiol. 2013 Jun 1;304(11):G1013-24 - PubMed
  5. Cell Metab. 2009 Sep;10(3):167-77 - PubMed
  6. Immunology. 2013 May;139(1):19-29 - PubMed
  7. Front Physiol. 2016 Dec 26;7:646 - PubMed
  8. Drug Discov Today. 2009 May;14(9-10):523-30 - PubMed
  9. Biochem Biophys Res Commun. 2005 Apr 1;329(1):386-90 - PubMed
  10. Clin Chem. 1977 Mar;23(3):576-80 - PubMed
  11. Mol Pharmacol. 2001 Dec;60(6):1243-53 - PubMed
  12. J Biol Chem. 2003 Mar 14;278(11):9435-40 - PubMed
  13. Ann N Y Acad Sci. 2002 Jun;968:49-64 - PubMed
  14. Mol Syst Biol. 2008;4:214 - PubMed
  15. Int Immunopharmacol. 2011 Dec;11(12):2150-8 - PubMed
  16. J Hepatol. 2011 Jun;54(6):1263-72 - PubMed
  17. Biol Pharm Bull. 2011;34(1):1-7 - PubMed
  18. Cell Signal. 2008 Dec;20(12):2180-97 - PubMed
  19. Eur J Pharmacol. 2016 Sep 5;786:109-115 - PubMed
  20. Mol Cell. 1999 May;3(5):543-53 - PubMed
  21. Biochem Biophys Res Commun. 2002 Nov 15;298(5):714-9 - PubMed
  22. Genes Dev. 2006 Jul 1;20(13):1817-28 - PubMed
  23. Cell Metab. 2011 Dec 7;14(6):747-57 - PubMed

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