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You T, Ding Y, Chen H, et al. Development of competitive and noncompetitive immunoassays for clothianidin with high sensitivity and specificity using phage-displayed peptides. J Hazard Mater. 2021;425:128011doi: 10.1016/j.jhazmat.2021.128011.
You, T., Ding, Y., Chen, H., Song, G., Huang, L., Wang, M., & Hua, X. (2021). Development of competitive and noncompetitive immunoassays for clothianidin with high sensitivity and specificity using phage-displayed peptides. Journal of hazardous materials, 425128011. https://doi.org/10.1016/j.jhazmat.2021.128011
You, Tianyang, et al. "Development of competitive and noncompetitive immunoassays for clothianidin with high sensitivity and specificity using phage-displayed peptides." Journal of hazardous materials vol. 425 (2021): 128011. doi: https://doi.org/10.1016/j.jhazmat.2021.128011
You T, Ding Y, Chen H, Song G, Huang L, Wang M, Hua X. Development of competitive and noncompetitive immunoassays for clothianidin with high sensitivity and specificity using phage-displayed peptides. J Hazard Mater. 2021 Dec 07;425:128011. doi: 10.1016/j.jhazmat.2021.128011. Epub 2021 Dec 07. PMID: 34896720.
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J Hazard Mater. 2021 Dec 07;425:128011. doi: 10.1016/j.jhazmat.2021.128011. Epub 2021 Dec 07.

Development of competitive and noncompetitive immunoassays for clothianidin with high sensitivity and specificity using phage-displayed peptides.

Journal of hazardous materials

Tianyang You, Yuan Ding, He Chen, Guangyue Song, Lianrun Huang, Minghua Wang, Xiude Hua

Affiliations

  1. College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China.
  2. College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China. Electronic address: [email protected].

PMID: 34896720 DOI: 10.1016/j.jhazmat.2021.128011

Abstract

Clothianidin is a widely used pesticide that has been banned from outdoor use by the European Union due to its toxicity. To improve the sensitivity and specificity of existing clothianidin immunoassays, we developed competitive and noncompetitive immunoassays for clothianidin based on phage-displayed peptides. Cyclic 8-, 9-, and 10-residue peptide libraries were constructed using an optimized phagemid pComb-pVIII to prevent the loss of theoretical library diversity. Twenty-eight peptidomimetics and two anti-immunocomplex peptides were isolated through a blended panning process and used to develop competitive and noncompetitive phage enzyme-linked immunosorbent assays (P-ELISAs), respectively. After optimization, the half inhibition concentration (IC

Copyright © 2021 Elsevier B.V. All rights reserved.

Keywords: Anti-immunocomplex peptide; Clothianidin; Noncompetitive immunoassay; Peptidomimetic; Phage display

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