Display options
Cite
Anderson MT, Brown AN, Pirani A, et al. Replication Dynamics for Six Gram-Negative Bacterial Species during Bloodstream Infection. mBio. 2021;12(4):e0111421doi: 10.1128/mBio.01114-21.
Anderson, M. T., Brown, A. N., Pirani, A., Smith, S. N., Photenhauer, A. L., Sun, Y., Snitkin, E. S., Bachman, M. A., & Mobley, H. L. T. (2021). Replication Dynamics for Six Gram-Negative Bacterial Species during Bloodstream Infection. mBio, 12(4), e0111421. https://doi.org/10.1128/mBio.01114-21
Anderson, Mark T, et al. "Replication Dynamics for Six Gram-Negative Bacterial Species during Bloodstream Infection." mBio vol. 12,4 (2021): e0111421. doi: https://doi.org/10.1128/mBio.01114-21
Anderson MT, Brown AN, Pirani A, Smith SN, Photenhauer AL, Sun Y, Snitkin ES, Bachman MA, Mobley HLT. Replication Dynamics for Six Gram-Negative Bacterial Species during Bloodstream Infection. mBio. 2021 Aug 31;12(4):e0111421. doi: 10.1128/mBio.01114-21. Epub 2021 Jul 06. PMID: 34225485; PMCID: PMC8406280.
Copy Download .nbib Format: NLM
Share it on

mBio. 2021 Aug 31;12(4):e0111421. doi: 10.1128/mBio.01114-21. Epub 2021 Jul 06.

Replication Dynamics for Six Gram-Negative Bacterial Species during Bloodstream Infection.

mBio

Mark T Anderson, Aric N Brown, Ali Pirani, Sara N Smith, Amanda L Photenhauer, Yuang Sun, Evan S Snitkin, Michael A Bachman, Harry L T Mobley

Affiliations

  1. University of Michigan Medical School, Department of Microbiology and Immunology, Ann Arbor, Michigan, USA.
  2. University of Michigan Medical School, Department of Pathology, Ann Arbor, Michigan, USA.
  3. University of Michigan Medical School, Division of Infectious Diseases, Ann Arbor, Michigan, USA.

PMID: 34225485 PMCID: PMC8406280 DOI: 10.1128/mBio.01114-21

Abstract

Bloodstream infections (BSI) are a major public health burden due to high mortality rates and the cost of treatment. The impact of BSI is further compounded by a rise in antibiotic resistance among Gram-negative species associated with these infections. Escherichia coli, Serratia marcescens, Klebsiella pneumoniae, Enterobacter hormaechei, Citrobacter freundii, and Acinetobacter baumannii are all common causes of BSI, which can be recapitulated in a murine model. The objective of this study was to characterize infection kinetics and bacterial replication rates during bacteremia for these six pathogens to gain a better understanding of bacterial physiology during infection. Temporal observations of bacterial burdens of the tested species demonstrated varied abilities to establish colonization in the spleen, liver, or kidney. K. pneumoniae and S. marcescens expanded rapidly in the liver and kidney, respectively. Other organisms, such as C. freundii and E. hormaechei, were steadily cleared from all three target organs throughout the infection.

Keywords: bacteremia; bloodstream infection; generation time; growth rate

References

  1. Nat Commun. 2018 Jun 20;9(1):2412 - PubMed
  2. Clin Infect Dis. 2018 Jan 18;66(3):460-463 - PubMed
  3. Clin Microbiol Infect. 2013 Jun;19(6):501-9 - PubMed
  4. mBio. 2017 May 23;8(3): - PubMed
  5. Sci Adv. 2019 Dec 11;5(12):eaax5727 - PubMed
  6. J Intern Med. 2020 Mar;287(3):283-300 - PubMed
  7. Nat Methods. 2012 Mar 04;9(4):357-9 - PubMed
  8. Clin Microbiol Infect. 2011 Mar;17(3):451-8 - PubMed
  9. Am J Med. 2010 Sep;123(9):819-28 - PubMed
  10. Antimicrob Agents Chemother. 2006 Dec;50(12):4114-23 - PubMed
  11. J Bacteriol. 1951 Sep;62(3):293-300 - PubMed
  12. PLoS Genet. 2013;9(10):e1003834 - PubMed
  13. Sci Rep. 2018 Aug 7;8(1):11792 - PubMed
  14. Infect Immun. 1990 May;58(5):1281-9 - PubMed
  15. BMC Genomics. 2019 Mar 20;20(1):229 - PubMed
  16. Mol Microbiol. 2006 Jul;61(2):310-23 - PubMed
  17. Bioinformatics. 2014 Aug 1;30(15):2114-20 - PubMed
  18. Nat Biotechnol. 2016 Dec;34(12):1256-1263 - PubMed
  19. mBio. 2010 Nov 23;1(5): - PubMed
  20. Antimicrob Agents Chemother. 2017 Feb 23;61(3): - PubMed
  21. Genome Res. 2017 Apr;27(4):601-612 - PubMed
  22. Mol Microbiol. 2018 Sep;109(6):745-762 - PubMed
  23. Pediatr Crit Care Med. 2013 Sep;14(7):686-93 - PubMed
  24. Nat Rev Immunol. 2017 Jul;17(7):407-420 - PubMed
  25. Clin Microbiol Infect. 2016 Mar;22(3):244-51 - PubMed
  26. J Microbiol Immunol Infect. 2006 Apr;39(2):135-43 - PubMed
  27. Genome Biol. 2014 Mar 03;15(3):R46 - PubMed
  28. Clin Microbiol Infect. 2013 Sep;19(9):860-8 - PubMed
  29. Science. 2015 Sep 4;349(6252):1101-1106 - PubMed
  30. Nature. 2002 Dec 19-26;420(6917):885-91 - PubMed
  31. Antimicrob Agents Chemother. 2019 Jun 24;63(7): - PubMed
  32. Nat Commun. 2018 Nov 23;9(1):4956 - PubMed
  33. J Clin Microbiol. 2011 May;49(5):1866-71 - PubMed
  34. J Microbiol Immunol Infect. 2018 Aug;51(4):565-572 - PubMed
  35. mSphere. 2015 Nov 04;1(1): - PubMed
  36. Sci Rep. 2018 Oct 8;8(1):14961 - PubMed
  37. Lancet. 2020 Jan 18;395(10219):200-211 - PubMed
  38. mBio. 2015 Jun 09;6(3):e00775 - PubMed
  39. Curr Protoc Bioinformatics. 2014 Sep 08;47:11.12.1-34 - PubMed
  40. mBio. 2018 Mar 6;9(2): - PubMed
  41. Bioinformatics. 2009 Aug 15;25(16):2078-9 - PubMed
  42. PLoS Pathog. 2013;9(12):e1003788 - PubMed
  43. Nat Rev Microbiol. 2020 Jun;18(6):344-359 - PubMed
  44. Infect Immun. 2020 Mar 23;88(4): - PubMed
  45. Appl Environ Microbiol. 2020 Jun 17;86(13): - PubMed
  46. PLoS Pathog. 2019 Aug 26;15(8):e1008010 - PubMed
  47. Clin Infect Dis. 1997 Apr;24(4):584-602 - PubMed
  48. Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):17020-4 - PubMed
  49. Microbiol Spectr. 2015 Aug;3(4): - PubMed
  50. Genome Announc. 2014 Sep 25;2(5): - PubMed
  51. J Bacteriol. 2018 Mar 12;200(7): - PubMed

Publication Types

Grant support