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Tsuchiya K, Goshima G. Microtubule-associated proteins promote microtubule generation in the absence of γ-tubulin in human colon cancer cells. J Cell Biol. 2021;220(12)doi: 10.1083/jcb.202104114.
Tsuchiya, K., & Goshima, G. (2021). Microtubule-associated proteins promote microtubule generation in the absence of γ-tubulin in human colon cancer cells. The Journal of cell biology, 220(12), . https://doi.org/10.1083/jcb.202104114
Tsuchiya, Kenta, and Goshima, Gohta. "Microtubule-associated proteins promote microtubule generation in the absence of γ-tubulin in human colon cancer cells." The Journal of cell biology vol. 220,12 (2021). doi: https://doi.org/10.1083/jcb.202104114
Tsuchiya K, Goshima G. Microtubule-associated proteins promote microtubule generation in the absence of γ-tubulin in human colon cancer cells. J Cell Biol. 2021 Dec 06;220(12). doi: 10.1083/jcb.202104114. Epub 2021 Nov 15. PMID: 34779859; PMCID: PMC8598081.
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J Cell Biol. 2021 Dec 06;220(12). doi: 10.1083/jcb.202104114. Epub 2021 Nov 15.

Microtubule-associated proteins promote microtubule generation in the absence of γ-tubulin in human colon cancer cells.

The Journal of cell biology

Kenta Tsuchiya, Gohta Goshima

Affiliations

  1. Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, Japan.
  2. Sugashima Marine Biological Laboratory, Graduate School of Science, Nagoya University, Nagoya, Japan.

PMID: 34779859 PMCID: PMC8598081 DOI: 10.1083/jcb.202104114

Abstract

The γ-tubulin complex acts as the predominant microtubule (MT) nucleator that initiates MT formation and is therefore an essential factor for cell proliferation. Nonetheless, cellular MTs are formed after experimental depletion of the γ-tubulin complex, suggesting that cells possess other factors that drive MT nucleation. Here, by combining gene knockout, auxin-inducible degron, RNA interference, MT depolymerization/regrowth assay, and live microscopy, we identified four microtubule-associated proteins (MAPs), ch-TOG, CLASP1, CAMSAPs, and TPX2, which are involved in γ-tubulin-independent MT generation in human colon cancer cells. In the mitotic MT regrowth assay, nucleated MTs organized noncentriolar MT organizing centers (ncMTOCs) in the absence of γ-tubulin. Depletion of CLASP1 or TPX2 substantially delayed ncMTOC formation, suggesting that these proteins might promote MT nucleation in the absence of γ-tubulin. In contrast, depletion of ch-TOG or CAMSAPs did not affect the timing of ncMTOC appearance. CLASP1 also accelerates γ-tubulin-independent MT regrowth during interphase. Thus, MT generation can be promoted by MAPs without the γ-tubulin template.

© 2021 Tsuchiya and Goshima.

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