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Veshkini A, M Hammon H, Vogel L, et al. Liver proteome profiling in dairy cows during the transition from gestation to lactation: Effects of supplementation with essential fatty acids and conjugated linoleic acids as explored by PLS-DA. J Proteomics. 2021;252:104436doi: 10.1016/j.jprot.2021.104436.
Veshkini, A., M Hammon, H., Vogel, L., Delosière, M., Viala, D., Dèjean, S., Tröscher, A., Ceciliani, F., Sauerwein, H., & Bonnet, M. (2021). Liver proteome profiling in dairy cows during the transition from gestation to lactation: Effects of supplementation with essential fatty acids and conjugated linoleic acids as explored by PLS-DA. Journal of proteomics, 252104436. https://doi.org/10.1016/j.jprot.2021.104436
Veshkini, Arash, et al. "Liver proteome profiling in dairy cows during the transition from gestation to lactation: Effects of supplementation with essential fatty acids and conjugated linoleic acids as explored by PLS-DA." Journal of proteomics vol. 252 (2021): 104436. doi: https://doi.org/10.1016/j.jprot.2021.104436
Veshkini A, M Hammon H, Vogel L, Delosière M, Viala D, Dèjean S, Tröscher A, Ceciliani F, Sauerwein H, Bonnet M. Liver proteome profiling in dairy cows during the transition from gestation to lactation: Effects of supplementation with essential fatty acids and conjugated linoleic acids as explored by PLS-DA. J Proteomics. 2021 Nov 25;252:104436. doi: 10.1016/j.jprot.2021.104436. Epub 2021 Nov 25. PMID: 34839038.
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J Proteomics. 2021 Nov 25;252:104436. doi: 10.1016/j.jprot.2021.104436. Epub 2021 Nov 25.

Liver proteome profiling in dairy cows during the transition from gestation to lactation: Effects of supplementation with essential fatty acids and conjugated linoleic acids as explored by PLS-DA.

Journal of proteomics

Arash Veshkini, Harald M Hammon, Laura Vogel, Mylène Delosière, Didier Viala, Sèbastien Dèjean, Arnulf Tröscher, Fabrizio Ceciliani, Helga Sauerwein, Muriel Bonnet

Affiliations

  1. Institute of Animal Science, Physiology Unit, University of Bonn, Bonn, Germany; Research Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany; INRAE, Université Clermont Auvergne, VetAgro Sup, UMR Herbivores, F-63122 Saint-Genès-Champanelle, France; Department of Veterinary Medicine, Università degli Studi di Milano, Lodi, Italy.
  2. Research Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany. Electronic address: [email protected].
  3. Research Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany.
  4. INRAE, Université Clermont Auvergne, VetAgro Sup, UMR Herbivores, F-63122 Saint-Genès-Champanelle, France.
  5. Institut de Mathématiques de Toulouse, UMR5219, Université de Toulouse, CNRS, UPS, 31062 Toulouse, France.
  6. BASF SE, 68623 Lampertheim, Germany.
  7. Department of Veterinary Medicine, Università degli Studi di Milano, Lodi, Italy.
  8. Institute of Animal Science, Physiology Unit, University of Bonn, Bonn, Germany.
  9. INRAE, Université Clermont Auvergne, VetAgro Sup, UMR Herbivores, F-63122 Saint-Genès-Champanelle, France. Electronic address: [email protected].

PMID: 34839038 DOI: 10.1016/j.jprot.2021.104436

Abstract

This study aimed at investigating the synergistic effects of essential fatty acids (EFA) and conjugated linoleic acids (CLA) on the liver proteome profile of dairy cows during the transition to lactation. 16 Holstein cows were infused from 9 wk. antepartum to 9 wk. postpartum into the abomasum with either coconut oil (CTRL) or a mixture of EFA (linseed + safflower oil) and CLA (EFA + CLA). Label-free quantitative proteomics was performed in liver tissue biopsied at days -21, +1, +28, and + 63 relative to calving. Differentially abundant proteins (DAP) between treatment groups were identified at the intersection between a multivariate and a univariate analysis. In total, 1680 proteins were identified at each time point, of which between groups DAP were assigned to the metabolism of xenobiotics by cytochrome P450, drug metabolism - cytochrome P450, steroid hormone biosynthesis, glycolysis/gluconeogenesis, and glutathione metabolism. Cytochrome P450, as a central hub, enriched with specific CYP enzymes comprising: CYP51A1 (d - 21), CYP1A1 & CYP4F2 (d + 28), and CYP4V2 (d + 63). Collectively, supplementation of EFA + CLA in transition cows impacted hepatic lipid metabolism and enriched several common biological pathways at all time points that were mainly related to ω-oxidation of fatty acids through the Cytochrome p450 pathway. SIGNIFICANCE: In three aspects this manuscript is notable. First, this is among the first longitudinal proteomics studies in nutrition of dairy cows. The selected time points are critical periods around parturition with profound endocrine and metabolic adaptations. Second, our findings provided novel information on key drivers of biologically relevant pathways suggested according to previously reported performance, zootechnical, and metabolism data (already published elsewhere). Third, our results revealed the role of cytochrome P450 that is hardly investigated, and of ω-oxidation pathways in the metabolism of fatty acids with the involvement of specific enzymes.

Copyright © 2021 Elsevier B.V. All rights reserved.

Keywords: Cytochrome p450; Fatty acid oxidation; Gene ontology; Liver proteome; Negative energy balance; Postpartum

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