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Charvoz L, Apothéloz-Perret-Gentil L, Reo E, et al. Monitoring newt communities in urban area using eDNA metabarcoding. PeerJ. 2021;9:e12357doi: 10.7717/peerj.12357.
Charvoz, L., Apothéloz-Perret-Gentil, L., Reo, E., Thiébaud, J., & Pawlowski, J. (2021). Monitoring newt communities in urban area using eDNA metabarcoding. PeerJ, 9e12357. https://doi.org/10.7717/peerj.12357
Charvoz, Léo, et al. "Monitoring newt communities in urban area using eDNA metabarcoding." PeerJ vol. 9 (2021): e12357. doi: https://doi.org/10.7717/peerj.12357
Charvoz L, Apothéloz-Perret-Gentil L, Reo E, Thiébaud J, Pawlowski J. Monitoring newt communities in urban area using eDNA metabarcoding. PeerJ. 2021 Nov 26;9:e12357. doi: 10.7717/peerj.12357. eCollection 2021. PMID: 34900410; PMCID: PMC8628619.
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PeerJ. 2021 Nov 26;9:e12357. doi: 10.7717/peerj.12357. eCollection 2021.

Monitoring newt communities in urban area using eDNA metabarcoding.

PeerJ

Léo Charvoz, Laure Apothéloz-Perret-Gentil, Emanuela Reo, Jacques Thiébaud, Jan Pawlowski

Affiliations

  1. Department of Genetics and Evolution, University of Geneva, Geneva, Geneva, Switzerland.
  2. ID-Gene ecodiagnostics, Campus Biotech Innovation Park, Geneva, Switzerland.
  3. KARCH-GE (Swiss Coordination Center for the Protection of Amphibians and Reptiles)-Geneva Regional Branch, Switzerland, Geneva, Geneva, Switzerland.
  4. Polish Academy of Sciences, Institute of Oceanology, Sopot, Pomerania, Poland.

PMID: 34900410 PMCID: PMC8628619 DOI: 10.7717/peerj.12357

Abstract

Newts are amphibians commonly present in small ponds or garden pools in urban areas. They are protected in many countries and their presence is monitored through visual observation and/or trapping. However, newts are not easy to spot as they are small, elusive and often hidden at the bottom of water bodies. In recent years, environmental DNA (eDNA) has become a popular tool for detecting newts, with a focus on individual species using qPCR assays. Here, we assess the effectiveness of eDNA metabarcoding compared to conventional visual surveys of newt diversity in 45 ponds within urban areas of Geneva canton, Switzerland. We designed newt-specific mitochondrial 16S rRNA primers, which assign the majority of amplicons to newts, and were able to detect four species known to be present in the region, including the invasive subspecies

©2021 Charvoz et al.

Keywords: 16S; Illumina; Invasive; Metabarcoding; Monitoring; Newt; Water; eDNA

Conflict of interest statement

Laure Apothéloz-Perret-Gentil and Jan Pawlowski are employed by ID-Gene ecodiagnostics, Switzerland.

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