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Anderson MT, Brown AN, Pirani A, et al. Replication Dynamics for Six Gram-Negative Bacterial Species during Bloodstream Infection. mBio. 2021;12(4):e0111421doi: 10.1128/mBio.01114-21.
Anderson, M. T., Brown, A. N., Pirani, A., Smith, S. N., Photenhauer, A. L., Sun, Y., Snitkin, E. S., Bachman, M. A., & Mobley, H. L. T. (2021). Replication Dynamics for Six Gram-Negative Bacterial Species during Bloodstream Infection. mBio, 12(4), e0111421. https://doi.org/10.1128/mBio.01114-21
Anderson, Mark T, et al. "Replication Dynamics for Six Gram-Negative Bacterial Species during Bloodstream Infection." mBio vol. 12,4 (2021): e0111421. doi: https://doi.org/10.1128/mBio.01114-21
Anderson MT, Brown AN, Pirani A, Smith SN, Photenhauer AL, Sun Y, Snitkin ES, Bachman MA, Mobley HLT. Replication Dynamics for Six Gram-Negative Bacterial Species during Bloodstream Infection. mBio. 2021 Aug 31;12(4):e0111421. doi: 10.1128/mBio.01114-21. Epub 2021 Jul 06. PMID: 34225485; PMCID: PMC8406280.
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mBio. 2021 Aug 31;12(4):e0111421. doi: 10.1128/mBio.01114-21. Epub 2021 Jul 06.

Replication Dynamics for Six Gram-Negative Bacterial Species during Bloodstream Infection.

mBio

Mark T Anderson, Aric N Brown, Ali Pirani, Sara N Smith, Amanda L Photenhauer, Yuang Sun, Evan S Snitkin, Michael A Bachman, Harry L T Mobley

Affiliations

  1. University of Michigan Medical School, Department of Microbiology and Immunology, Ann Arbor, Michigan, USA.
  2. University of Michigan Medical School, Department of Pathology, Ann Arbor, Michigan, USA.
  3. University of Michigan Medical School, Division of Infectious Diseases, Ann Arbor, Michigan, USA.

PMID: 34225485 PMCID: PMC8406280 DOI: 10.1128/mBio.01114-21

Abstract

Bloodstream infections (BSI) are a major public health burden due to high mortality rates and the cost of treatment. The impact of BSI is further compounded by a rise in antibiotic resistance among Gram-negative species associated with these infections. Escherichia coli, Serratia marcescens, Klebsiella pneumoniae, Enterobacter hormaechei, Citrobacter freundii, and Acinetobacter baumannii are all common causes of BSI, which can be recapitulated in a murine model. The objective of this study was to characterize infection kinetics and bacterial replication rates during bacteremia for these six pathogens to gain a better understanding of bacterial physiology during infection. Temporal observations of bacterial burdens of the tested species demonstrated varied abilities to establish colonization in the spleen, liver, or kidney. K. pneumoniae and S. marcescens expanded rapidly in the liver and kidney, respectively. Other organisms, such as C. freundii and E. hormaechei, were steadily cleared from all three target organs throughout the infection.

Keywords: bacteremia; bloodstream infection; generation time; growth rate

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