J Appl Biomed. 2021 Sep;19(3):149-158. doi: 10.32725/jab.2021.017. Epub 2021 Aug 10.

Effect of the natural flavonoids myricetin and dihydromyricetin on the wound healing process in vitro.

Journal of applied biomedicine

Renata Sklenarova, Marika Svrckova, Petr Hodek, Jitka Ulrichova, Jana Frankova

PMID: 34907758 DOI: 10.32725/jab.2021.017

Abstract

Myricetin (MYR) and dihydromyricetin (DHM) are classified as natural flavonoids. Both substances are known for their anti-inflammatory and antioxidant properties. In this study, an in vitro model of inflammation was demonstrated on monolayers of scratched fibroblasts or keratinocytes exposed to LPS from Pseudomonas aeruginosa for six hours. MYR and DHM were subsequently applied to the cells for 24 hours at sub toxic concentrations (5-15 µM). Inflammatory parameters were analysed in collected cell medium and lysate after the incubation period using the Enzyme-Linked ImmuneSorbent Assay (ELISA) and Western blot. Both flavonoids inhibit the production of pro-inflammatory cytokines (IL-6, IL-8) in LPS-stimulated skin cells as well as the decreased level of MMP-1 in fibroblasts. However, the application of MYR and DHM dose dependently increased the level of MMP-1 in keratinocytes. In our experiments, we focused on the anti-glycation activity of MYR and DHM, where the higher concentration of MYR seems to be more effective.

Keywords: Dihydromyricetin; Inflammation; Myricetin; Skin wound healing

Conflict of interest statement

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Publication Types

• Journal Article

Grant support

• IGA_LF_2019_015/Czech Science Foundation/Czech Republic
• IGA_LF_2021_011/Czech Science Foundation/Czech Republic
• P303/12/G163/Czech Science Foundation/Czech Republic