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Consonni SR, de Carvalho ACPV, Mauro AB, et al. Lentiviral transduction of neonatal rat ventricular myocytes preserves ultrastructural features of genetically modified cells. Virology. 2021;562:190-196doi: 10.1016/j.virol.2021.07.015.
Consonni, S. R., de Carvalho, A. C. P. V., Mauro, A. B., Franchini, K. G., & Bajgelman, M. C. (2021). Lentiviral transduction of neonatal rat ventricular myocytes preserves ultrastructural features of genetically modified cells. Virology, 562190-196. https://doi.org/10.1016/j.virol.2021.07.015
Consonni, Sílvio R, et al. "Lentiviral transduction of neonatal rat ventricular myocytes preserves ultrastructural features of genetically modified cells." Virology vol. 562 (2021): 190-196. doi: https://doi.org/10.1016/j.virol.2021.07.015
Consonni SR, de Carvalho ACPV, Mauro AB, Franchini KG, Bajgelman MC. Lentiviral transduction of neonatal rat ventricular myocytes preserves ultrastructural features of genetically modified cells. Virology. 2021 Oct;562:190-196. doi: 10.1016/j.virol.2021.07.015. Epub 2021 Jul 31. PMID: 34365094.
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Virology. 2021 Oct;562:190-196. doi: 10.1016/j.virol.2021.07.015. Epub 2021 Jul 31.

Lentiviral transduction of neonatal rat ventricular myocytes preserves ultrastructural features of genetically modified cells.

Virology

Sílvio R Consonni, Anna C P V de Carvalho, Artur B Mauro, Kleber G Franchini, Marcio C Bajgelman

Affiliations

  1. Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil.
  2. Brazilian National Laboratory for Biosciences (LNBio), Center for Research in Energy and Materials (CNPEM), Campinas, SP, Brazil.
  3. Brazilian National Laboratory for Biosciences (LNBio), Center for Research in Energy and Materials (CNPEM), Campinas, SP, Brazil; Medical School, University of Campinas (UNICAMP), Campinas, SP, Brazil.
  4. Brazilian National Laboratory for Biosciences (LNBio), Center for Research in Energy and Materials (CNPEM), Campinas, SP, Brazil; Faculty of Pharmaceutical Sciences, University of Campinas (UNICAMP), Campinas, SP, Brazil; Medical School, University of Campinas (UNICAMP), Campinas, SP, Brazil. Electronic address: [email protected].

PMID: 34365094 DOI: 10.1016/j.virol.2021.07.015

Abstract

Preserving morphological features that are important for cell function and structure is a critical parameter for in vitro experiments with rat cardiomyocytes. Lentiviral vectors are commonly used as gene transfer tool because of its high flexibility, efficiency to deliver expression cassettes and versatility of transducing quiescent cells. The tropism of the recombinant viral particle can be determined depending on the virus envelope, which shows a specific binding to cell surface receptors on the target cell. The combination of promoter arrangement and viral envelope must be optimized to achieve a greater transduction efficiency and a higher transgene expression. In this study we explored the optimization of promoters and heterologous envelopes to transduce primary culture of neonatal rat ventricular myocytes. Our results suggest a robust expression driven by the cytomegalovirus promoter, and high efficiency transduction mediated by VSV-G envelope with no apparent compromising ultrastructural features of genetically modified cells.

Copyright © 2021 Elsevier Inc. All rights reserved.

Keywords: Cardiomyocyte; Gene transfer; Lentivirus; Pseudotyping; Superinfection interference

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