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Liu S, Lu W, Ma S, et al. Comparison of the neutralizing activities of antibodies in clinical sera against both Sabin and wild-type polio pseudoviruses. J Virol Methods. 2021;300:114376doi: 10.1016/j.jviromet.2021.114376.
Liu, S., Lu, W., Ma, S., Guo, H., Zhang, Z., & Li, X. (2021). Comparison of the neutralizing activities of antibodies in clinical sera against both Sabin and wild-type polio pseudoviruses. Journal of virological methods, 300114376. https://doi.org/10.1016/j.jviromet.2021.114376
Liu, Shaohua, et al. "Comparison of the neutralizing activities of antibodies in clinical sera against both Sabin and wild-type polio pseudoviruses." Journal of virological methods vol. 300 (2021): 114376. doi: https://doi.org/10.1016/j.jviromet.2021.114376
Liu S, Lu W, Ma S, Guo H, Zhang Z, Li X. Comparison of the neutralizing activities of antibodies in clinical sera against both Sabin and wild-type polio pseudoviruses. J Virol Methods. 2021 Nov 24;300:114376. doi: 10.1016/j.jviromet.2021.114376. Epub 2021 Nov 24. PMID: 34826519.
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J Virol Methods. 2021 Nov 24;300:114376. doi: 10.1016/j.jviromet.2021.114376. Epub 2021 Nov 24.

Comparison of the neutralizing activities of antibodies in clinical sera against both Sabin and wild-type polio pseudoviruses.

Journal of virological methods

Shaohua Liu, Weiwei Lu, Shuhua Ma, Huijie Guo, Zhongyang Zhang, Xiuling Li

Affiliations

  1. National Vaccine & Serum Institute, Beijing, China.
  2. National Vaccine & Serum Institute, Beijing, China; Shanghai Institute of Biological Products Co. LTD, China. Electronic address: [email protected].

PMID: 34826519 DOI: 10.1016/j.jviromet.2021.114376

Abstract

The cost-effectiveness of the Sabin inactivated poliovirus vaccine derived from the Sabin strains (S-IPV) and its reduced biosecurity risks during its manufacture make it the vaccine of choice over the IPVs derived from wild-type polioviruses. However, it is difficult to evaluate whether S-IPVs can achieve wild-type poliovirus containment in China, making its development there less attractive. To facilitate the development and adoption of S-IPVs in China, the aim of this study was to develop an alternative neutralizing assay using either a polio pseudovirus derived from a Sabin strain (S-pNA) or one derived from a wild-type strain (w-pNA) to replace the conventional neutralizing assay which uses live polioviruses. A total of 100 sera were collected from children immunized with an oral poliovirus vaccine and their antibody titers were assessed by both the S-pNA and w-pNA. The results showed that this method was feasible for the quantification of neutralizing antibody activities in the sera of the vaccinated individuals. The Wilcoxon signed-rank sum test indicated that the neutralizing antibody titers obtained against the Sabin strains were higher than those obtained with the wild-type strains for types 1 and 3, while for type 2, the titers against the wild-type strains were higher than those against the Sabin strains (p < 0.001 for all three types). It is hoped that this assay could be used to assess whether immune sera by the S-IPV possess adequate neutralizing capacity against both attenuated and wild-type poliovirus strains.

Copyright © 2021. Published by Elsevier B.V.

Keywords: Neutralization assay; Neutralizing antibody; Poliovirus; Pseudovirus

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