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Grigorieva DV, Gorudko IV, Grudinina NA, et al. Lactoferrin modified by hypohalous acids: Partial loss in activation of human neutrophils. Int J Biol Macromol. 2021;195:30-40doi: 10.1016/j.ijbiomac.2021.11.165.
Grigorieva, D. V., Gorudko, I. V., Grudinina, N. A., Panasenko, O. M., Semak, I. V., Sokolov, A. V., & Timoshenko, A. V. (2021). Lactoferrin modified by hypohalous acids: Partial loss in activation of human neutrophils. International journal of biological macromolecules, 19530-40. https://doi.org/10.1016/j.ijbiomac.2021.11.165
Grigorieva, Daria V, et al. "Lactoferrin modified by hypohalous acids: Partial loss in activation of human neutrophils." International journal of biological macromolecules vol. 195 (2021): 30-40. doi: https://doi.org/10.1016/j.ijbiomac.2021.11.165
Grigorieva DV, Gorudko IV, Grudinina NA, Panasenko OM, Semak IV, Sokolov AV, Timoshenko AV. Lactoferrin modified by hypohalous acids: Partial loss in activation of human neutrophils. Int J Biol Macromol. 2021 Dec 01;195:30-40. doi: 10.1016/j.ijbiomac.2021.11.165. Epub 2021 Dec 01. PMID: 34863835.
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Int J Biol Macromol. 2021 Dec 01;195:30-40. doi: 10.1016/j.ijbiomac.2021.11.165. Epub 2021 Dec 01.

Lactoferrin modified by hypohalous acids: Partial loss in activation of human neutrophils.

International journal of biological macromolecules

Daria V Grigorieva, Irina V Gorudko, Natalia A Grudinina, Oleg M Panasenko, Igor V Semak, Alexey V Sokolov, Alexander V Timoshenko

Affiliations

  1. Department of Biophysics, Faculty of Physics, Belarusian State University, Minsk 220030, Belarus.
  2. Department of Biophysics, Faculty of Physics, Belarusian State University, Minsk 220030, Belarus. Electronic address: [email protected].
  3. Laboratory of Biochemical Genetics, Department of Molecular Genetics, FSBRI "Institute of Experimental Medicine", St. Petersburg 197376, Russia.
  4. Department of Biophysics, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow 119435, Russia.
  5. Department of Biochemistry, Faculty of Biology, Belarusian State University, Minsk 220030, Belarus.
  6. Laboratory of Biochemical Genetics, Department of Molecular Genetics, FSBRI "Institute of Experimental Medicine", St. Petersburg 197376, Russia; Department of Biophysics, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow 119435, Russia.
  7. Department of Biology, The University of Western Ontario, London, ON N6A 5B7, Canada.

PMID: 34863835 DOI: 10.1016/j.ijbiomac.2021.11.165

Abstract

Previously we have shown that lactoferrin (LTF), a protein of secondary neutrophilic granules, can be efficiently modified by hypohalous acids (HOCl and HOBr), which are produced at high concentrations during inflammation and oxidative/halogenative stress by myeloperoxidase, an enzyme of azurophilic neutrophilic granules. Here we compared the effects of recombinant human lactoferrin (rhLTF) and its halogenated derivatives (rhLTF-Cl and rhLTF-Br) on functional responses of neutrophils. Our results demonstrated that after halogenative modification, rhLTF lost its ability to induce mobilization of intracellular calcium, actin cytoskeleton reorganization, and morphological changes in human neutrophils. Moreover, both forms of the halogenated rhLTF prevented binding of N-acetylglucosamine-specific plant lectin Triticum vulgaris agglutinin (WGA) to neutrophils and, in contrast to native rhLTF, inhibited respiratory burst of neutrophils induced by N-formyl-L-methionyl-L-leucyl-L-phenylalanine and by two plant lectins (WGA and PHA-L). However, we observed no differences between the effects of rhLTF, rhLTF-Cl, and rhLTF-Br on respiratory burst of neutrophils induced by phorbol 12-myristate 13-acetate (PMA), digitonin, and number of plant lectins with different glycan-binding specificity. Furthermore, all rhLTF forms interfered with PMA- and ionomycin-induced formation of neutrophil extracellular traps. Thus, halogenative modification of LTF is one of the mechanisms involved in modulating a variety of signaling pathways in neutrophils to control their pro-inflammatory activity.

Copyright © 2021 Elsevier B.V. All rights reserved.

Keywords: Hypohalous acids; Lactoferrin; Neutrophils

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