Display options
Cite
Paloschi V, Sabater-Lleal M, Middelkamp H, et al. Organ-on-a-chip technology: a novel approach to investigate cardiovascular diseases. Cardiovasc Res. 2021;117(14):2742-2754doi: 10.1093/cvr/cvab088.
Paloschi, V., Sabater-Lleal, M., Middelkamp, H., Vivas, A., Johansson, S., van der Meer, A., Tenje, M., & Maegdefessel, L. (2021). Organ-on-a-chip technology: a novel approach to investigate cardiovascular diseases. Cardiovascular research, 117(14), 2742-2754. https://doi.org/10.1093/cvr/cvab088
Paloschi, Valentina, et al. "Organ-on-a-chip technology: a novel approach to investigate cardiovascular diseases." Cardiovascular research vol. 117,14 (2021): 2742-2754. doi: https://doi.org/10.1093/cvr/cvab088
Paloschi V, Sabater-Lleal M, Middelkamp H, Vivas A, Johansson S, van der Meer A, Tenje M, Maegdefessel L. Organ-on-a-chip technology: a novel approach to investigate cardiovascular diseases. Cardiovasc Res. 2021 Dec 17;117(14):2742-2754. doi: 10.1093/cvr/cvab088. PMID: 33729461; PMCID: PMC8683705.
Copy Download .nbib Format: NLM
Share it on

Cardiovasc Res. 2021 Dec 17;117(14):2742-2754. doi: 10.1093/cvr/cvab088.

Organ-on-a-chip technology: a novel approach to investigate cardiovascular diseases.

Cardiovascular research

Valentina Paloschi, Maria Sabater-Lleal, Heleen Middelkamp, Aisen Vivas, Sofia Johansson, Andries van der Meer, Maria Tenje, Lars Maegdefessel

Affiliations

  1. Department for Vascular and Endovascular Surgery, Technical University Munich, Klinikum Rechts der Isar, Munich, Germany.
  2. German Center for Cardiovascular Research (DZHK), Partner Site Munich Heart Alliance, Berlin, Germany.
  3. Research Institute of Hospital de la Santa Creu i Sant Pau, IIB Sant Pau, Genomics of Complex Diseases Group, Barcelona, Spain.
  4. Cardiovascular Medicine Unit, Department of Medicine, Karolinska Institutet, Stockholm, Sweden.
  5. BIOS/Lab on a Chip, University of Twente, Enschede, The Netherlands.
  6. Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands.
  7. Department of Materials Science and Engineering, Science for Life Laboratory, Uppsala University, Uppsala, Sweden.
  8. Molecular Vascular Medicine Unit, Department of Medicine, Karolinska Institutet, Stockholm, Sweden.

PMID: 33729461 PMCID: PMC8683705 DOI: 10.1093/cvr/cvab088

Abstract

The development of organs-on-chip (OoC) has revolutionized in vitro cell-culture experiments by allowing a better mimicry of human physiology and pathophysiology that has consequently led researchers to gain more meaningful insights into disease mechanisms. Several models of hearts-on-chips and vessels-on-chips have been demonstrated to recapitulate fundamental aspects of the human cardiovascular system in the recent past. These 2D and 3D systems include synchronized beating cardiomyocytes in hearts-on-chips and vessels-on-chips with layer-based structures and the inclusion of physiological and pathological shear stress conditions. The opportunities to discover novel targets and to perform drug testing with chip-based platforms have substantially enhanced, thanks to the utilization of patient-derived cells and precise control of their microenvironment. These organ models will provide an important asset for future approaches to personalized cardiovascular medicine and improved patient care. However, certain technical and biological challenges remain, making the global utilization of OoCs to tackle unanswered questions in cardiovascular science still rather challenging. This review article aims to introduce and summarize published work on hearts- and vessels-on chips but also to provide an outlook and perspective on how these advanced in vitro systems can be used to tailor disease models with patient-specific characteristics.

© The Author(s) 2021. Published by Oxford University Press on behalf of the European Society of Cardiology.

Keywords: Cardiovascular; Cell culture; Heart; Organs-on-chips; Personalized medicine

References

  1. Proc Natl Acad Sci U S A. 2013 Jan 22;110(4):1357-62 - PubMed
  2. Lab Chip. 2017 Dec 19;18(1):115-125 - PubMed
  3. Biosens Bioelectron. 2018 Jul 30;112:149-155 - PubMed
  4. Lab Chip. 2017 Jun 27;17(13):2294-2302 - PubMed
  5. Nat Protoc. 2019 Nov;14(11):3082-3100 - PubMed
  6. JACC Heart Fail. 2018 Mar;6(3):179-186 - PubMed
  7. Anal Chem Insights. 2015 Nov 29;10:39-45 - PubMed
  8. Adv Healthc Mater. 2019 Feb;8(3):e1801146 - PubMed
  9. Am J Manag Care. 2017 Dec;23(20 Suppl):S376-S382 - PubMed
  10. Nat Cell Biol. 2020 Mar;22(3):321-331 - PubMed
  11. Talanta. 2020 Nov 1;219:121269 - PubMed
  12. Proc Natl Acad Sci U S A. 2017 Mar 21;114(12):E2293-E2302 - PubMed
  13. Physiol Rev. 2013 Jan;93(1):327-58 - PubMed
  14. Cell Stem Cell. 2016 Dec 1;19(6):703-708 - PubMed
  15. Arterioscler Thromb Vasc Biol. 2017 Nov;37(11):2014-2025 - PubMed
  16. Lab Chip. 2019 Jan 15;19(2):206-213 - PubMed
  17. J Mol Cell Cardiol. 1986 Jul;18(7):661-78 - PubMed
  18. Lab Chip. 2019 Jul 23;19(15):2500-2511 - PubMed
  19. Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):3179-84 - PubMed
  20. Nat Rev Cardiol. 2020 Jun;17(6):341-359 - PubMed
  21. Genes Dev. 2010 Oct 15;24(20):2239-63 - PubMed
  22. Adv Drug Deliv Rev. 2020;165-166:60-76 - PubMed
  23. PLoS One. 2016 Mar 01;11(3):e0150360 - PubMed
  24. Adv Mater. 2019 Dec;31(50):e1902042 - PubMed
  25. Circulation. 2016 Aug 9;134(6):e32-69 - PubMed
  26. Adv Funct Mater. 2020 Mar 17;30(12): - PubMed
  27. Circulation. 2010 Dec 21;122(25):2727-35 - PubMed
  28. Cell Mol Life Sci. 2014 Jun;71(12):2271-88 - PubMed
  29. ALTEX. 2019;36(4):650-668 - PubMed
  30. Lab Chip. 2015 Jun 21;15(12):2688-99 - PubMed
  31. J Lab Autom. 2012 Apr;17(2):96-103 - PubMed
  32. Lab Chip. 2013 Sep 21;13(18):3599-608 - PubMed
  33. Circulation. 2013 Jul 23;128(4):388-400 - PubMed
  34. Lancet. 2012 Dec 15;380(9859):2095-128 - PubMed
  35. Biomicrofluidics. 2017 Mar 16;11(2):024105 - PubMed
  36. Biochem Biophys Res Commun. 2017 Jan 8;482(2):323-328 - PubMed
  37. Toxicol Sci. 2020 Jul 1;176(1):103-123 - PubMed
  38. Lab Chip. 2019 Apr 9;19(8):1448-1457 - PubMed
  39. APL Bioeng. 2019 Jun 21;3(2):026105 - PubMed
  40. ACS Biomater Sci Eng. 2020 Sep 14;6(9):5326-5336 - PubMed
  41. Lab Chip. 2011 Jul 7;11(13):2175-81 - PubMed
  42. Biomaterials. 2020 Mar;233:119741 - PubMed
  43. Ther Innov Regul Sci. 2019 Jul;53(4):519-525 - PubMed
  44. Lab Chip. 2010 Feb 7;10(3):291-302 - PubMed
  45. Nat Commun. 2017 Aug 15;8(1):262 - PubMed
  46. Adv Drug Deliv Rev. 2016 Jan 15;96:253-73 - PubMed
  47. Thromb Res. 2001 Feb 15;101(4):321-7 - PubMed
  48. J Clin Invest. 2012 Jan;122(1):408-18 - PubMed
  49. ACS Biomater Sci Eng. 2021 Jul 12;7(7):2880-2899 - PubMed
  50. Sci Rep. 2015 Mar 09;5:8883 - PubMed
  51. Lab Chip. 2014 Aug 7;14(15):2709-16 - PubMed
  52. Proc Natl Acad Sci U S A. 2012 Jun 12;109(24):9342-7 - PubMed
  53. Tissue Eng Part C Methods. 2020 Jan;26(1):44-55 - PubMed
  54. Cell Stem Cell. 2016 May 5;18(5):573-86 - PubMed
  55. Lab Chip. 2010 Sep 21;10(18):2341-9 - PubMed
  56. EMBO Rep. 2017 Mar;18(3):367-376 - PubMed
  57. Biomed Microdevices. 2016 Aug;18(4):73 - PubMed
  58. Nat Commun. 2016 Jan 06;7:10176 - PubMed
  59. Clin Pharmacol Ther. 2018 Dec;104(6):1240-1248 - PubMed
  60. Am J Med. 2005 May;118(5):544-7 - PubMed
  61. Anal Chem. 2019 Jan 2;91(1):330-351 - PubMed
  62. Sci Rep. 2017 Aug 15;7(1):8168 - PubMed
  63. Nat Biomed Eng. 2018;2:453-463 - PubMed
  64. J Mater Chem B. 2016 May 28;4(20):3534-3543 - PubMed
  65. Lab Chip. 2012 Jun 21;12(12):2156-64 - PubMed
  66. Blood. 2019 Nov 7;134(19):1645-1657 - PubMed
  67. Semin Immunopathol. 2012 Jan;34(1):93-106 - PubMed
  68. Nat Genet. 2017 Sep;49(9):1385-1391 - PubMed
  69. Cardiovasc Drugs Ther. 2017 Jun;31(3):325-344 - PubMed
  70. Micromachines (Basel). 2019 Nov 14;10(11): - PubMed
  71. Nat Med. 2009 Jun;15(6):665-73 - PubMed
  72. Cell Stem Cell. 2018 Mar 1;22(3):310-324 - PubMed
  73. Adv Healthc Mater. 2019 Jul;8(13):e1900435 - PubMed
  74. Biosens Bioelectron. 2016 Jan 15;75:67-81 - PubMed
  75. Circ Res. 2009 Feb 27;104(4):e30-41 - PubMed
  76. Nat Protoc. 2012 Jun 07;7(7):1247-59 - PubMed
  77. Tissue Eng Part A. 2019 May;25(9-10):786-798 - PubMed
  78. Elife. 2019 Aug 27;8: - PubMed
  79. Integr Biol (Camb). 2016 Jun 13;8(6):672-83 - PubMed
  80. Viruses. 2019 Jan 30;11(2): - PubMed
  81. Nat Rev Mol Cell Biol. 2007 Oct;8(10):839-45 - PubMed
  82. Nat Commun. 2020 Jan 9;11(1):163 - PubMed
  83. Circ Res. 2017 Apr 28;120(9):1487-1500 - PubMed
  84. ACS Sens. 2019 Oct 25;4(10):2623-2630 - PubMed
  85. Nat Rev Cardiol. 2019 Dec;16(12):727-744 - PubMed
  86. Biomaterials. 2019 Apr;198:3-26 - PubMed
  87. Nat Genet. 2019 Nov;51(11):1574-1579 - PubMed
  88. Stem Cell Reports. 2017 Nov 14;9(5):1560-1572 - PubMed
  89. J Thromb Haemost. 2020 Apr;18(4):931-941 - PubMed
  90. Lab Chip. 2015 Jun 7;15(11):2379-87 - PubMed
  91. Sci Transl Med. 2019 Nov 6;11(517): - PubMed
  92. Platelets. 2012;23(7):501-9 - PubMed
  93. J Natl Cancer Inst. 2010 Jan 6;102(1):14-25 - PubMed
  94. Lab Chip. 2020 Feb 21;20(4):749-759 - PubMed
  95. J Cell Biochem. 2018 Jan;119(1):62-68 - PubMed
  96. Front Med (Lausanne). 2019 Nov 15;6:265 - PubMed
  97. Lab Chip. 2013 Jul 7;13(13):2591-8 - PubMed
  98. Macromol Biosci. 2019 Jun;19(6):e1900060 - PubMed
  99. Biorheology. 2015;52(5-6):303-18 - PubMed
  100. Cell. 2014 Jun 5;157(6):1262-1278 - PubMed
  101. Lab Chip. 2017 Aug 8;17(16):2785-2792 - PubMed
  102. Anal Chem. 1998 Dec 1;70(23):4974-84 - PubMed
  103. Nat Rev Dis Primers. 2019 Aug 16;5(1):56 - PubMed
  104. Stem Cells Dev. 2009 Jan-Feb;18(1):161-72 - PubMed
  105. STAR Protoc. 2020 Jun 03;1(1):100026 - PubMed
  106. Nat Rev Mol Cell Biol. 2016 Mar;17(3):183-93 - PubMed
  107. Biomaterials. 2020 Oct;256:120195 - PubMed
  108. BMC Bioinformatics. 2018 Nov 6;19(1):407 - PubMed
  109. Sci Rep. 2016 Feb 03;6:20030 - PubMed
  110. Lab Chip. 2019 Jan 15;19(2):198-205 - PubMed
  111. Mater Sci Eng C Mater Biol Appl. 2017 Dec 1;81:84-89 - PubMed
  112. Lab Chip. 2016 Feb 7;16(3):599-610 - PubMed
  113. Biomark Insights. 2015 May 27;10(Suppl 1):71-6 - PubMed
  114. Stem Cell Rev Rep. 2017 Jun;13(3):335-346 - PubMed
  115. Pathophysiol Haemost Thromb. 2003 Sep-2004 Dec;33(5-6):455-7 - PubMed
  116. Lab Chip. 2013 Aug 7;13(15):2990-8 - PubMed
  117. Stem Cell Rev Rep. 2015 Jun;11(3):511-25 - PubMed
  118. Lab Chip. 2013 Apr 21;13(8):1489-500 - PubMed
  119. Cell. 2020 May 14;181(4):905-913.e7 - PubMed
  120. Biomed Microdevices. 2015 Oct;17(5):88 - PubMed
  121. Lab Chip. 2017 May 16;17(10):1732-1739 - PubMed
  122. ALTEX. 2019;36(3):481-492 - PubMed
  123. Circ Res. 2019 Oct 25;125(10):e75-e92 - PubMed
  124. Cell Stem Cell. 2013 Dec 5;13(6):653-8 - PubMed
  125. Nat Med. 2014 Jun;20(6):616-23 - PubMed
  126. Nat Genet. 2018 Apr;50(4):524-537 - PubMed
  127. Appl In Vitro Toxicol. 2016 Jun 1;2(2):82-96 - PubMed
  128. Sci Rep. 2015 Jul 23;5:12401 - PubMed
  129. Eur Heart J. 2016 Nov 7;37(42):3232-3245 - PubMed
  130. Lab Chip. 2017 Sep 26;17(19):3264-3271 - PubMed
  131. Nano Lett. 2020 Apr 8;20(4):2585-2593 - PubMed
  132. Microsyst Nanoeng. 2020 Mar 9;6:23 - PubMed

Publication Types

Grant support