Display options
Cite
Shanbhag S, Kampleitner C, Mohamed-Ahmed S, et al. Ectopic Bone Tissue Engineering in Mice Using Human Gingiva or Bone Marrow-Derived Stromal/Progenitor Cells in Scaffold-Hydrogel Constructs. Front Bioeng Biotechnol. 2021;9:783468doi: 10.3389/fbioe.2021.783468.
Shanbhag, S., Kampleitner, C., Mohamed-Ahmed, S., Yassin, M. A., Dongre, H., Costea, D. E., Tangl, S., Stavropoulos, A., Bolstad, A. I., Suliman, S., & Mustafa, K. (2021). Ectopic Bone Tissue Engineering in Mice Using Human Gingiva or Bone Marrow-Derived Stromal/Progenitor Cells in Scaffold-Hydrogel Constructs. Frontiers in bioengineering and biotechnology, 9783468. https://doi.org/10.3389/fbioe.2021.783468
Shanbhag, Siddharth, et al. "Ectopic Bone Tissue Engineering in Mice Using Human Gingiva or Bone Marrow-Derived Stromal/Progenitor Cells in Scaffold-Hydrogel Constructs." Frontiers in bioengineering and biotechnology vol. 9 (2021): 783468. doi: https://doi.org/10.3389/fbioe.2021.783468
Shanbhag S, Kampleitner C, Mohamed-Ahmed S, Yassin MA, Dongre H, Costea DE, Tangl S, Stavropoulos A, Bolstad AI, Suliman S, Mustafa K. Ectopic Bone Tissue Engineering in Mice Using Human Gingiva or Bone Marrow-Derived Stromal/Progenitor Cells in Scaffold-Hydrogel Constructs. Front Bioeng Biotechnol. 2021 Nov 30;9:783468. doi: 10.3389/fbioe.2021.783468. eCollection 2021. PMID: 34917602; PMCID: PMC8670384.
Copy Download .nbib Format: NLM
Share it on

Front Bioeng Biotechnol. 2021 Nov 30;9:783468. doi: 10.3389/fbioe.2021.783468. eCollection 2021.

Ectopic Bone Tissue Engineering in Mice Using Human Gingiva or Bone Marrow-Derived Stromal/Progenitor Cells in Scaffold-Hydrogel Constructs.

Frontiers in bioengineering and biotechnology

Siddharth Shanbhag, Carina Kampleitner, Samih Mohamed-Ahmed, Mohammed Ahmad Yassin, Harsh Dongre, Daniela Elena Costea, Stefan Tangl, Andreas Stavropoulos, Anne Isine Bolstad, Salwa Suliman, Kamal Mustafa

Affiliations

  1. Center for Translational Oral Research (TOR), Department of Clinical Dentistry, Faculty of Medicine, University of Bergen, Bergen, Norway.
  2. Department of Immunology and Transfusion Medicine, Haukeland University Hospital, Bergen, Norway.
  3. Ludwig Boltzmann Institute for Traumatology, The Research Center in Cooperation With AUVA, Vienna, Austria.
  4. Karl Donath Laboratory for Hard Tissue and Biomaterial Research, University Clinic of Dentistry, Medical University of Vienna, Vienna, Austria.
  5. Austrian Cluster for Tissue Regeneration, Vienna, Austria.
  6. Gade Laboratory for Pathology, Department of Clinical Medicine, Faculty of Medicine, University of Bergen, Bergen, Norway.
  7. Centre for Cancer Biomarkers (CCBIO), Faculty of Medicine, University of Bergen, Bergen, Norway.
  8. Department of Periodontology, Faculty of Odontology, Malmö University, Malmö, Sweden.
  9. Division of Conservative Dentistry and Periodontology, University Clinic of Dentistry, Medical University of Vienna, Vienna, Austria.

PMID: 34917602 PMCID: PMC8670384 DOI: 10.3389/fbioe.2021.783468

Abstract

Three-dimensional (3D) spheroid culture can promote the osteogenic differentiation and bone regeneration capacity of mesenchymal stromal cells (MSC). Gingiva-derived progenitor cells (GPC) represent a less invasive alternative to bone marrow MSC (BMSC) for clinical applications. The aim of this study was to test the

Copyright © 2021 Shanbhag, Kampleitner, Mohamed-Ahmed, Yassin, Dongre, Costea, Tangl, Stavropoulos, Bolstad, Suliman and Mustafa.

Keywords: MSc; bone tissue engineering; platelet lysate; spheroid culture; xeno-free

Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

References

  1. Stem Cell Res Ther. 2017 Apr 26;8(1):94 - PubMed
  2. Cytotherapy. 2012 May;14(5):540-54 - PubMed
  3. Stem Cell Res Ther. 2020 Sep 23;11(1):351 - PubMed
  4. Stem Cells Dev. 2012 Mar 20;21(5):655-67 - PubMed
  5. Stem Cell Res Ther. 2013 Jan 04;4(1):1 - PubMed
  6. Clin Implant Dent Relat Res. 2015 Jan;17 Suppl 1:e17-34 - PubMed
  7. Stem Cells. 2008 Sep;26(9):2287-99 - PubMed
  8. Stem Cell Res Ther. 2017 Dec 6;8(1):275 - PubMed
  9. Tissue Eng Part A. 2010 Sep;16(9):2891-9 - PubMed
  10. J Tissue Eng. 2020 Sep 16;11:2041731420954316 - PubMed
  11. Tissue Eng Part B Rev. 2016 Aug;22(4):322-9 - PubMed
  12. Sci Rep. 2019 Nov 5;9(1):16031 - PubMed
  13. Acta Biomater. 2013 May;9(5):6630-40 - PubMed
  14. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003 May;95(5):521-8 - PubMed
  15. Cells Tissues Organs. 2006;183(2):68-79 - PubMed
  16. Acta Haematol. 2016;136(2):85-97 - PubMed
  17. Stem Cells Transl Med. 2016 Jun;5(6):773-81 - PubMed
  18. J Periodontal Res. 2018 Oct;53(5):870-882 - PubMed
  19. Exp Hematol. 2007 Jun;35(6):995-1004 - PubMed
  20. PLoS Biol. 2010 Jun 29;8(6):e1000412 - PubMed
  21. J Craniomaxillofac Surg. 2003 Feb;31(1):27-33 - PubMed
  22. Stem Cells. 2018 Sep;36(9):1393-1403 - PubMed
  23. Bioessays. 2000 Feb;22(2):138-47 - PubMed
  24. Sci Rep. 2017 Dec 4;7(1):16878 - PubMed
  25. BMC Musculoskelet Disord. 2021 Apr 30;22(1):401 - PubMed
  26. Calcif Tissue Int. 2013 Mar;92(3):296-306 - PubMed
  27. Tissue Eng Part A. 2020 Aug;26(15-16):852-862 - PubMed
  28. J Clin Periodontol. 2019 Jun;46 Suppl 21:162-182 - PubMed
  29. Eur Cell Mater. 2017 Feb 15;33:121-129 - PubMed
  30. Stem Cell Res Ther. 2018 Jun 19;9(1):168 - PubMed
  31. Tissue Eng Part B Rev. 2014 Oct;20(5):365-80 - PubMed
  32. Cytotherapy. 2006;8(4):315-7 - PubMed
  33. Eur Cell Mater. 2011 Jun 20;21:488-507 - PubMed
  34. Int J Oral Maxillofac Surg. 2011 Sep;40(9):961-5 - PubMed
  35. Acta Biomater. 2019 Jun;91:1-23 - PubMed
  36. Development. 2016 Jul 1;143(13):2273-80 - PubMed
  37. Ann Biomed Eng. 2015 Aug;43(8):2010-21 - PubMed
  38. Transfusion. 2019 Nov;59(11):3448-3460 - PubMed
  39. Dent Clin North Am. 2022 Jan;66(1):111-130 - PubMed
  40. Acta Biomater. 2016 May;36:86-98 - PubMed
  41. Front Bioeng Biotechnol. 2020 Aug 19;8:968 - PubMed
  42. Stem Cells Int. 2016;2016:9176357 - PubMed
  43. Clin Oral Implants Res. 2018 Apr;29(4):381-388 - PubMed
  44. Nat Biotechnol. 2000 Sep;18(9):954-8 - PubMed
  45. Int J Med Sci. 2018 May 22;15(8):748-757 - PubMed
  46. J Struct Biol. 2013 Aug;183(2):258-69 - PubMed
  47. Int J Implant Dent. 2021 Jun 7;7(1):50 - PubMed
  48. NPJ Regen Med. 2019 Dec 2;4:22 - PubMed
  49. BMC Biotechnol. 2014 Dec 06;14:105 - PubMed
  50. Cell Tissue Res. 2014 Jul;357(1):91-9 - PubMed
  51. Nat Commun. 2018 Oct 9;9(1):4170 - PubMed
  52. Biomaterials. 2008 Oct;29(30):4091-9 - PubMed
  53. Biomed Mater. 2014 Aug 28;9(5):055005 - PubMed
  54. J Tissue Eng Regen Med. 2018 Jan;12(1):e473-e485 - PubMed
  55. J Periodontol. 2010 Jun;81(6):917-25 - PubMed
  56. J Orthop Res. 2019 Jun;37(6):1318-1328 - PubMed
  57. Biomaterials. 2014 Dec;35(36):9660-7 - PubMed
  58. J Clin Periodontol. 2012 Feb;39 Suppl 12:1-5 - PubMed
  59. Stem Cell Res Ther. 2014 Oct 13;5(5):114 - PubMed
  60. Biochem Biophys Res Commun. 2010 Mar 12;393(3):377-83 - PubMed
  61. Oral Surg Oral Med Oral Pathol Oral Radiol. 2012 Nov;114(5 Suppl):S32-40 - PubMed
  62. Front Immunol. 2020 Feb 18;11:243 - PubMed
  63. Stem Cells Transl Med. 2015 Apr;4(4):412 - PubMed
  64. Tissue Eng Part B Rev. 2017 Dec;23(6):552-569 - PubMed

Publication Types