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Yang B, Minne M, Brunoni F, et al. Non-cell autonomous and spatiotemporal signalling from a tissue organizer orchestrates root vascular development. Nat Plants. 2021;7(11):1485-1494doi: 10.1038/s41477-021-01017-6.
Yang, B., Minne, M., Brunoni, F., Plačková, L., Petřík, I., Sun, Y., Nolf, J., Smet, W., Verstaen, K., Wendrich, J. R., Eekhout, T., Hoyerová, K., Van Isterdael, G., Haustraete, J., Bishopp, A., Farcot, E., Novák, O., Saeys, Y., & De Rybel, B. (2021). Non-cell autonomous and spatiotemporal signalling from a tissue organizer orchestrates root vascular development. Nature plants, 7(11), 1485-1494. https://doi.org/10.1038/s41477-021-01017-6
Yang, BaoJun, et al. "Non-cell autonomous and spatiotemporal signalling from a tissue organizer orchestrates root vascular development." Nature plants vol. 7,11 (2021): 1485-1494. doi: https://doi.org/10.1038/s41477-021-01017-6
Yang B, Minne M, Brunoni F, Plačková L, Petřík I, Sun Y, Nolf J, Smet W, Verstaen K, Wendrich JR, Eekhout T, Hoyerová K, Van Isterdael G, Haustraete J, Bishopp A, Farcot E, Novák O, Saeys Y, De Rybel B. Non-cell autonomous and spatiotemporal signalling from a tissue organizer orchestrates root vascular development. Nat Plants. 2021 Nov;7(11):1485-1494. doi: 10.1038/s41477-021-01017-6. Epub 2021 Nov 15. PMID: 34782768.
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Nat Plants. 2021 Nov;7(11):1485-1494. doi: 10.1038/s41477-021-01017-6. Epub 2021 Nov 15.

Non-cell autonomous and spatiotemporal signalling from a tissue organizer orchestrates root vascular development.

Nature plants

BaoJun Yang, Max Minne, Federica Brunoni, Lenka Plačková, Ivan Petřík, Yanbiao Sun, Jonah Nolf, Wouter Smet, Kevin Verstaen, Jos R Wendrich, Thomas Eekhout, Klára Hoyerová, Gert Van Isterdael, Jurgen Haustraete, Anthony Bishopp, Etienne Farcot, Ondřej Novák, Yvan Saeys, Bert De Rybel

Affiliations

  1. Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium. [email protected].
  2. VIB Center for Plant Systems Biology, Ghent, Belgium. [email protected].
  3. Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.
  4. VIB Center for Plant Systems Biology, Ghent, Belgium.
  5. Laboratory of Growth Regulators, Institute of Experimental Botany of the Czech Academy of Sciences and Faculty of Science of Palacký University, Olomouc, Czech Republic.
  6. Department of Applied Mathematics, Computer Science and Statistics, Ghent University, Ghent, Belgium.
  7. VIB Center for Inflammation Research, Data Mining and Modelling for Biomedicine, Ghent, Belgium.
  8. Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany, Czech Academy of Sciences, Prague, Czech Republic.
  9. VIB Flow Core, VIB Center for Inflammation Research, Ghent, Belgium.
  10. Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
  11. VIB Protein Service Facility, VIB Center for Inflammation Research, Ghent, Belgium.
  12. School of Biosciences, University of Nottingham, Loughborough, UK.
  13. School of Mathematical Sciences, University of Nottingham, Nottingham, UK.
  14. Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium. [email protected].
  15. VIB Center for Plant Systems Biology, Ghent, Belgium. [email protected].

PMID: 34782768 DOI: 10.1038/s41477-021-01017-6

Abstract

During plant development, a precise balance of cytokinin is crucial for correct growth and patterning, but it remains unclear how this is achieved across different cell types and in the context of a growing organ. Here we show that in the root apical meristem, the TMO5/LHW complex increases active cytokinin levels via two cooperatively acting enzymes. By profiling the transcriptomic changes of increased cytokinin at single-cell level, we further show that this effect is counteracted by a tissue-specific increase in CYTOKININ OXIDASE 3 expression via direct activation of the mobile transcription factor SHORTROOT. In summary, we show that within the root meristem, xylem cells act as a local organizer of vascular development by non-autonomously regulating cytokinin levels in neighbouring procambium cells via sequential induction and repression modules.

© 2021. The Author(s), under exclusive licence to Springer Nature Limited.

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