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Buntenbroich I, Simões T, Escobar-Henriques M. Analysis of Protein Stability by Synthesis Shutoff. Bio Protoc. 2021;11(22):e4225doi: 10.21769/BioProtoc.4225.
Buntenbroich, I., Simões, T., & Escobar-Henriques, M. (2021). Analysis of Protein Stability by Synthesis Shutoff. Bio-protocol, 11(22), e4225. https://doi.org/10.21769/BioProtoc.4225
Buntenbroich, Ira, et al. "Analysis of Protein Stability by Synthesis Shutoff." Bio-protocol vol. 11,22 (2021): e4225. doi: https://doi.org/10.21769/BioProtoc.4225
Buntenbroich I, Simões T, Escobar-Henriques M. Analysis of Protein Stability by Synthesis Shutoff. Bio Protoc. 2021 Nov 20;11(22):e4225. doi: 10.21769/BioProtoc.4225. eCollection 2021 Nov 20. PMID: 34909446; PMCID: PMC8635844.
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Bio Protoc. 2021 Nov 20;11(22):e4225. doi: 10.21769/BioProtoc.4225. eCollection 2021 Nov 20.

Analysis of Protein Stability by Synthesis Shutoff.

Bio-protocol

Ira Buntenbroich, Tânia Simões, Mafalda Escobar-Henriques

Affiliations

  1. Institute for Genetics, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases, University of Cologne, Cologne, Germany.

PMID: 34909446 PMCID: PMC8635844 DOI: 10.21769/BioProtoc.4225

Abstract

In this protocol, we describe the analysis of protein stability over time, using synthesis shutoff. As an example, we express HA-tagged yeast mitofusin Fzo1 in

Copyright © The Authors; exclusive licensee Bio-protocol LLC.

Keywords: Cycloheximide chase; Fzo1; SDS-PAGE; Shutoff; Western blot

Conflict of interest statement

Competing interestsThe authors declare they have no conflict of interest or competing interests.

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