Display options
Cite
Hettinger ZR, Hamagata K, Confides AL, et al. Age-Related Susceptibility to Muscle Damage Following Mechanotherapy in Rats Recovering From Disuse Atrophy. J Gerontol A Biol Sci Med Sci. 2021;76(12):2132-2140doi: 10.1093/gerona/glab186.
Hettinger, Z. R., Hamagata, K., Confides, A. L., Lawrence, M. M., Miller, B. F., Butterfield, T. A., & Dupont-Versteegden, E. E. (2021). Age-Related Susceptibility to Muscle Damage Following Mechanotherapy in Rats Recovering From Disuse Atrophy. The journals of gerontology. Series A, Biological sciences and medical sciences, 76(12), 2132-2140. https://doi.org/10.1093/gerona/glab186
Hettinger, Zachary R, et al. "Age-Related Susceptibility to Muscle Damage Following Mechanotherapy in Rats Recovering From Disuse Atrophy." The journals of gerontology. Series A, Biological sciences and medical sciences vol. 76,12 (2021): 2132-2140. doi: https://doi.org/10.1093/gerona/glab186
Hettinger ZR, Hamagata K, Confides AL, Lawrence MM, Miller BF, Butterfield TA, Dupont-Versteegden EE. Age-Related Susceptibility to Muscle Damage Following Mechanotherapy in Rats Recovering From Disuse Atrophy. J Gerontol A Biol Sci Med Sci. 2021 Nov 15;76(12):2132-2140. doi: 10.1093/gerona/glab186. PMID: 34181006; PMCID: PMC8599051.
Copy Download .nbib Format: NLM
Share it on

J Gerontol A Biol Sci Med Sci. 2021 Nov 15;76(12):2132-2140. doi: 10.1093/gerona/glab186.

Age-Related Susceptibility to Muscle Damage Following Mechanotherapy in Rats Recovering From Disuse Atrophy.

The journals of gerontology. Series A, Biological sciences and medical sciences

Zachary R Hettinger, Kyoko Hamagata, Amy L Confides, Marcus M Lawrence, Benjamin F Miller, Timothy A Butterfield, Esther E Dupont-Versteegden

Affiliations

  1. Department of Physical Therapy, College of Health Sciences, University of Kentucky, Lexington, USA.
  2. Center for Muscle Biology, University of Kentucky, Lexington, USA.
  3. Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, Oklahoma City, USA.
  4. Department of Athletic Training and Clinical Nutrition, College of Health Sciences, University of Kentucky, Lexington, USA.

PMID: 34181006 PMCID: PMC8599051 DOI: 10.1093/gerona/glab186

Abstract

The inability to fully recover lost muscle mass following periods of disuse atrophy predisposes older adults to lost independence and poor quality of life. We have previously shown that mechanotherapy at a moderate load (4.5 N) enhances muscle mass recovery following atrophy in adult, but not older adult rats. We propose that elevated transverse stiffness in aged muscle inhibits the growth response to mechanotherapy and hypothesize that a higher load (7.6 N) will overcome this resistance to mechanical stimuli. F344/BN adult and older adult male rats underwent 14 days of hindlimb suspension, followed by 7 days of recovery with (RE + M) or without (RE) mechanotherapy at 7.6 N on gastrocnemius muscle. The 7.6 N load was determined by measuring transverse passive stiffness and linearly scaling up from 4.5 N. No differences in protein turnover or mean fiber cross-sectional area were observed between RE and RE + M for older adult rats or adult rats at 7.6 N. However, there was a higher number of small muscle fibers present in older adult, but not adult rats, which was explained by a 16-fold increase in the frequency of small fibers expressing embryonic myosin heavy chain. Elevated central nucleation, satellite cell abundance, and dystrophin-/laminin+ fibers were present in older adult rats only following 7.6 N, while 4.5 N did not induce damage at either age. We conclude that age is an important variable when considering load used during mechanotherapy and age-related transverse stiffness may predispose older adults to damage during the recovery period following disuse atrophy.

© The Author(s) 2021. Published by Oxford University Press on behalf of The Gerontological Society of America. All rights reserved. For permissions, please e-mail: [email protected].

Keywords: Aging; Disuse atrophy; Extracellular matrix; Mechanotherapy; Skeletal muscle

References

  1. J Appl Physiol (1985). 2019 Dec 1;127(6):1782-1791 - PubMed
  2. J Med Biol Eng. 2016;36:285-307 - PubMed
  3. Function (Oxf). 2020;1(1):zqaa009 - PubMed
  4. Int J Biochem Cell Biol. 2013 Oct;45(10):2200-8 - PubMed
  5. Biophys J. 2010 Oct 20;99(8):2434-42 - PubMed
  6. J Appl Physiol (1985). 2014 Aug 15;117(4):363-9 - PubMed
  7. Sports Med. 2005;35(5):413-27 - PubMed
  8. J Strength Cond Res. 2010 Oct;24(10):2857-72 - PubMed
  9. Curr Opin Clin Nutr Metab Care. 2010 Jan;13(1):34-9 - PubMed
  10. Age (Dordr). 2013 Dec;35(6):2203-14 - PubMed
  11. J Physiol. 2018 Jan 1;596(1):83-103 - PubMed
  12. Biochim Biophys Acta. 2006 May;1760(5):730-44 - PubMed
  13. J Cell Physiol. 2019 Dec;234(12):23547-23558 - PubMed
  14. J Cachexia Sarcopenia Muscle. 2019 Dec;10(6):1195-1209 - PubMed
  15. Sports (Basel). 2019 May 29;7(6): - PubMed
  16. J Gerontol A Biol Sci Med Sci. 2017 May 1;72(5):640-648 - PubMed
  17. Elife. 2018 Dec 19;7: - PubMed
  18. Ann Biomed Eng. 2014 Jan;42(1):36-49 - PubMed
  19. Physiol Rev. 2019 Jan 1;99(1):427-511 - PubMed
  20. J Bodyw Mov Ther. 2011 Jul;15(3):335-42 - PubMed
  21. J Appl Physiol (1985). 2005 Apr;98(4):1562-6 - PubMed
  22. Am J Physiol Cell Physiol. 2008 Jul;295(1):C249-56 - PubMed
  23. J Appl Physiol (1985). 2000 Mar;88(3):1112-8 - PubMed
  24. Exerc Sport Sci Rev. 2013 Jul;41(3):169-73 - PubMed
  25. J Gerontol A Biol Sci Med Sci. 2007 Apr;62(4):375-81 - PubMed
  26. J Strength Cond Res. 2019 Aug;33(8):2019-2052 - PubMed
  27. Circ Res. 2010 Sep 3;107(5):615-9 - PubMed
  28. J Appl Physiol (1985). 2014 Jan 15;116(2):164-75 - PubMed
  29. Am J Physiol Heart Circ Physiol. 2004 Aug;287(2):H645-51 - PubMed
  30. Exp Gerontol. 2015 Apr;64:17-32 - PubMed
  31. FEBS Lett. 2015 Jun 4;589(13):1491-7 - PubMed
  32. Exp Gerontol. 2014 Apr;52:1-8 - PubMed
  33. Front Physiol. 2019 Jan 14;9:1915 - PubMed
  34. Skelet Muscle. 2017 Nov 7;7(1):24 - PubMed
  35. J Appl Physiol (1985). 2015 Mar 15;118(6):655-61 - PubMed
  36. J Appl Physiol (1985). 2015 Aug 15;119(4):321-7 - PubMed
  37. J Gerontol A Biol Sci Med Sci. 2009 Jun;64(6):618-28 - PubMed
  38. J Anat. 1984 Dec;139 ( Pt 4):677-89 - PubMed
  39. Geroscience. 2021 Feb;43(1):65-83 - PubMed
  40. Sci STKE. 2002 Feb 12;2002(119):pe6 - PubMed
  41. Skelet Muscle. 2011 Mar 02;1(1):11 - PubMed
  42. Int J Mol Sci. 2020 Jun 02;21(11): - PubMed
  43. J Appl Physiol (1985). 2018 Jan 01;124(1):40-51 - PubMed
  44. Am J Physiol Cell Physiol. 2016 Oct 1;311(4):C616-C629 - PubMed
  45. PLoS One. 2019 Nov 5;14(11):e0224232 - PubMed
  46. Acta Physiol (Oxf). 2020 Jul;229(3):e13460 - PubMed
  47. J Appl Physiol (1985). 2007 Dec;103(6):2068-76 - PubMed
  48. FASEB J. 2006 Apr;20(6):768-9 - PubMed
  49. Aging (Albany NY). 2016 Jan;8(1):127-46 - PubMed

Publication Types

Grant support