Display options
Cite
Yu Y, Wen Q, Song A, et al. Isolation and immune activity of a new acidic Cordyceps militaris exopolysaccharide. Int J Biol Macromol. 2021;194:706-714doi: 10.1016/j.ijbiomac.2021.11.115.
Yu, Y., Wen, Q., Song, A., Liu, Y., Wang, F., & Jiang, B. (2022). Isolation and immune activity of a new acidic Cordyceps militaris exopolysaccharide. International journal of biological macromolecules, 194706-714. https://doi.org/10.1016/j.ijbiomac.2021.11.115
Yu, Yue, et al. "Isolation and immune activity of a new acidic Cordyceps militaris exopolysaccharide." International journal of biological macromolecules vol. 194 (2022): 706-714. doi: https://doi.org/10.1016/j.ijbiomac.2021.11.115
Yu Y, Wen Q, Song A, Liu Y, Wang F, Jiang B. Isolation and immune activity of a new acidic Cordyceps militaris exopolysaccharide. Int J Biol Macromol. 2022 Jan 01;194:706-714. doi: 10.1016/j.ijbiomac.2021.11.115. Epub 2021 Nov 20. PMID: 34813790.
Copy Download .nbib Format: NLM
Share it on

Int J Biol Macromol. 2022 Jan 01;194:706-714. doi: 10.1016/j.ijbiomac.2021.11.115. Epub 2021 Nov 20.

Isolation and immune activity of a new acidic Cordyceps militaris exopolysaccharide.

International journal of biological macromolecules

Yue Yu, Qiang Wen, Ao Song, Yang Liu, Fei Wang, Bin Jiang

Affiliations

  1. Graduate School of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, PR China.
  2. National Institutes for Food and Drug Control, Beijing 102629, PR China.
  3. Changchun Customs Technology Center, Changchun 130033, PR China.
  4. School of Life Science, Liaocheng University, Liaocheng 252059, PR China. Electronic address: [email protected].
  5. Graduate School of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, PR China. Electronic address: [email protected].

PMID: 34813790 DOI: 10.1016/j.ijbiomac.2021.11.115

Abstract

A new type of acidic exopolysaccharide (AESP-II) was extracted and separated from the fermentation broth of Cordyceps militaris (C. militaris), which was further purified to elucidate its structural characteristics and immunological activity. AESP-II was confirmed to be an acidic pyranose with a molecular weight of 61.52 kDa, which consisted of mannose, glucuronic acid, rhamnose, galactose acid, N-acetyl-galactosamine, glucose, galactose and arabinose with a molar ratio of 1.07: 5.38: 1: 3.14: 2.23: 15: 6.09: and 4.04. Animal experiment results verified that AESP-II can significantly promote the proliferation of spleen T and B lymphocytes in mice with immune injury caused by cyclophosphamide (CTX). In particular, the promotion of B lymphocytes presented a dose-effect relationship. In addition, the levels of the cytokines IL-2, IL-4, and IFN-γ, which are mainly secreted by T lymphocytes, and immunoglobulin IgG, IgM and IgA, which are mainly secreted by B lymphocytes, were increased after AESP-II treatment. The above results suggest that fluid immunity is involved in the immunomodulatory function of AESP-II. Simultaneously, AESP-II was detected significantly to promote the phosphorylation expression of p38 kinase (p38), extracellular regulated protein kinases (ERK) and c-Jun N-terminal kinase (JNK) by Western blot, further suggesting that the activation of the MAPK signaling pathway mediates the immunoregulatory function of AESP-II.

Copyright © 2021. Published by Elsevier B.V.

Keywords: Acidic exopolysaccharide; C. militaris strain; Fermentation broth; Immune activity; Polysaccharide structure

Publication Types