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Shanbhag S, Kampleitner C, Mohamed-Ahmed S, et al. Ectopic Bone Tissue Engineering in Mice Using Human Gingiva or Bone Marrow-Derived Stromal/Progenitor Cells in Scaffold-Hydrogel Constructs. Front Bioeng Biotechnol. 2021;9:783468doi: 10.3389/fbioe.2021.783468.
Shanbhag, S., Kampleitner, C., Mohamed-Ahmed, S., Yassin, M. A., Dongre, H., Costea, D. E., Tangl, S., Stavropoulos, A., Bolstad, A. I., Suliman, S., & Mustafa, K. (2021). Ectopic Bone Tissue Engineering in Mice Using Human Gingiva or Bone Marrow-Derived Stromal/Progenitor Cells in Scaffold-Hydrogel Constructs. Frontiers in bioengineering and biotechnology, 9783468. https://doi.org/10.3389/fbioe.2021.783468
Shanbhag, Siddharth, et al. "Ectopic Bone Tissue Engineering in Mice Using Human Gingiva or Bone Marrow-Derived Stromal/Progenitor Cells in Scaffold-Hydrogel Constructs." Frontiers in bioengineering and biotechnology vol. 9 (2021): 783468. doi: https://doi.org/10.3389/fbioe.2021.783468
Shanbhag S, Kampleitner C, Mohamed-Ahmed S, Yassin MA, Dongre H, Costea DE, Tangl S, Stavropoulos A, Bolstad AI, Suliman S, Mustafa K. Ectopic Bone Tissue Engineering in Mice Using Human Gingiva or Bone Marrow-Derived Stromal/Progenitor Cells in Scaffold-Hydrogel Constructs. Front Bioeng Biotechnol. 2021 Nov 30;9:783468. doi: 10.3389/fbioe.2021.783468. eCollection 2021. PMID: 34917602; PMCID: PMC8670384.
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Front Bioeng Biotechnol. 2021 Nov 30;9:783468. doi: 10.3389/fbioe.2021.783468. eCollection 2021.

Ectopic Bone Tissue Engineering in Mice Using Human Gingiva or Bone Marrow-Derived Stromal/Progenitor Cells in Scaffold-Hydrogel Constructs.

Frontiers in bioengineering and biotechnology

Siddharth Shanbhag, Carina Kampleitner, Samih Mohamed-Ahmed, Mohammed Ahmad Yassin, Harsh Dongre, Daniela Elena Costea, Stefan Tangl, Andreas Stavropoulos, Anne Isine Bolstad, Salwa Suliman, Kamal Mustafa

Affiliations

  1. Center for Translational Oral Research (TOR), Department of Clinical Dentistry, Faculty of Medicine, University of Bergen, Bergen, Norway.
  2. Department of Immunology and Transfusion Medicine, Haukeland University Hospital, Bergen, Norway.
  3. Ludwig Boltzmann Institute for Traumatology, The Research Center in Cooperation With AUVA, Vienna, Austria.
  4. Karl Donath Laboratory for Hard Tissue and Biomaterial Research, University Clinic of Dentistry, Medical University of Vienna, Vienna, Austria.
  5. Austrian Cluster for Tissue Regeneration, Vienna, Austria.
  6. Gade Laboratory for Pathology, Department of Clinical Medicine, Faculty of Medicine, University of Bergen, Bergen, Norway.
  7. Centre for Cancer Biomarkers (CCBIO), Faculty of Medicine, University of Bergen, Bergen, Norway.
  8. Department of Periodontology, Faculty of Odontology, Malmö University, Malmö, Sweden.
  9. Division of Conservative Dentistry and Periodontology, University Clinic of Dentistry, Medical University of Vienna, Vienna, Austria.

PMID: 34917602 PMCID: PMC8670384 DOI: 10.3389/fbioe.2021.783468

Abstract

Three-dimensional (3D) spheroid culture can promote the osteogenic differentiation and bone regeneration capacity of mesenchymal stromal cells (MSC). Gingiva-derived progenitor cells (GPC) represent a less invasive alternative to bone marrow MSC (BMSC) for clinical applications. The aim of this study was to test the

Copyright © 2021 Shanbhag, Kampleitner, Mohamed-Ahmed, Yassin, Dongre, Costea, Tangl, Stavropoulos, Bolstad, Suliman and Mustafa.

Keywords: MSc; bone tissue engineering; platelet lysate; spheroid culture; xeno-free

Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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