J Infect Dis. 2021 Apr 23;223(8):1488-1496. doi: 10.1093/infdis/jiaa548.
Hemolytic Membrane Vesicles of Group B Streptococcus Promote Infection.
The Journal of infectious diseases
Blair Armistead, Phoenicia Quach, Jessica M Snyder, Verónica Santana-Ufret, Anna Furuta, Alyssa Brokaw, Lakshmi Rajagopal
Affiliations
Affiliations
- Department of Global Health, University of Washington, Seattle, Washington, USA.
- Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA.
- Department of Comparative Medicine, University of Washington School of Medicine, Seattle, Washington, USA.
- Department of Pediatrics, University of Washington School of Medicine, Seattle, Washington, USA.
PMID: 32861213
PMCID: PMC8064051 DOI: 10.1093/infdis/jiaa548
Abstract
BACKGROUND: Group B streptococci (GBS) are β-hemolytic, Gram-positive bacteria associated with fetal injury, preterm birth, spontaneous abortion, and neonatal infections. A key factor promoting GBS virulence is the β-hemolysin/cytolysin, a pigmented ornithine rhamnolipid (also known as granadaene) associated with the bacterial surface.
METHODS: A previous study indicated that GBS produce small structures known as membrane vesicles (MVs), which contain virulence-associated proteins. In this study, we show that GBS MVs are pigmented and hemolytic, indicating that granadaene is functionally active in MVs.
RESULTS: In addition, MVs from hyperhemolytic GBS induced greater cell death of neutrophils, T cells, and B cells compared with MVs from isogenic nonhemolytic GBS, implicating MVs as a potential mechanism for granadaene-mediated virulence. Finally, hemolytic MVs reduced oxidative killing of GBS and aggravated morbidity and mortality of neonatal mice infected with GBS.
CONCLUSIONS: These studies, taken together, reveal a novel mechanism by which GBS deploy a crucial virulence factor to promote bacterial dissemination and pathogenesis.
© The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: [email protected].
Keywords: granadaene; group B streptococcus; hemolysin; immune evasion; membrane vesicles
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