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Devos L, Agnès F, Edouard J, et al. Eye morphogenesis in the blind Mexican cavefish. Biol Open. 2021;10(10)doi: 10.1242/bio.059031.
Devos, L., Agnès, F., Edouard, J., Simon, V., Legendre, L., El Khallouki, N., Barbachou, S., Sohm, F., & Rétaux, S. (2021). Eye morphogenesis in the blind Mexican cavefish. Biology open, 10(10), . https://doi.org/10.1242/bio.059031
Devos, Lucie, et al. "Eye morphogenesis in the blind Mexican cavefish." Biology open vol. 10,10 (2021). doi: https://doi.org/10.1242/bio.059031
Devos L, Agnès F, Edouard J, Simon V, Legendre L, El Khallouki N, Barbachou S, Sohm F, Rétaux S. Eye morphogenesis in the blind Mexican cavefish. Biol Open. 2021 Oct 15;10(10). doi: 10.1242/bio.059031. Epub 2021 Oct 28. PMID: 34590124; PMCID: PMC8565469.
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Biol Open. 2021 Oct 15;10(10). doi: 10.1242/bio.059031. Epub 2021 Oct 28.

Eye morphogenesis in the blind Mexican cavefish.

Biology open

Lucie Devos, François Agnès, Joanne Edouard, Victor Simon, Laurent Legendre, Naima El Khallouki, Sosthène Barbachou, Frédéric Sohm, Sylvie Rétaux

Affiliations

  1. Paris-Saclay Institute of Neuroscience, CNRS, Université Paris-Saclay, 91198 Gif sur Yvette, France.
  2. AMAGEN, CNRS, INRA, Université Paris-Saclay, 91198, Gif sur Yvette, France.

PMID: 34590124 PMCID: PMC8565469 DOI: 10.1242/bio.059031

Abstract

The morphogenesis of the vertebrate eye consists of a complex choreography of cell movements, tightly coupled to axial regionalization and cell type specification processes. Disturbances in these events can lead to developmental defects and blindness. Here, we have deciphered the sequence of defective events leading to coloboma in the embryonic eye of the blind cavefish of the species Astyanax mexicanus. Using comparative live imaging on targeted enhancer-trap Zic1:hsp70:GFP reporter lines of both the normal, river-dwelling morph and the cave morph of the species, we identified defects in migratory cell behaviours during evagination that participate in the reduced optic vesicle size in cavefish, without proliferation defect. Further, impaired optic cup invagination shifts the relative position of the lens and contributes to coloboma in cavefish. Based on these results, we propose a developmental scenario to explain the cavefish phenotype and discuss developmental constraints to morphological evolution. The cavefish eye appears as an outstanding natural mutant model to study molecular and cellular processes involved in optic region morphogenesis.

© 2021. Published by The Company of Biologists Ltd.

Keywords: Zic1 ; CRISPR/Cas9 knock-in; Cell behaviours; Evo-devo; Live imaging; Optic vesicle; Retinal pigmented epithelium

Conflict of interest statement

Competing interests The authors declare no competing or financial interests.

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