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Albarrán-Rodriguez RR, Castillo-Juarez H, Rivera-Benítez F, et al. Assessment of the hemagglutinating activity of the Porcine orthorubulavirus. Comp Immunol Microbiol Infect Dis. 2021;80:101736doi: 10.1016/j.cimid.2021.101736.
Albarrán-Rodriguez, R. R., Castillo-Juarez, H., Rivera-Benítez, F., Campos-Montes, G. R., Espinosa, B., Madrigal-Valencia, T. L., Jimenez, E. N. S., & Ramírez-Mendoza, H. (2021). Assessment of the hemagglutinating activity of the Porcine orthorubulavirus. Comparative immunology, microbiology and infectious diseases, 80101736. https://doi.org/10.1016/j.cimid.2021.101736
Albarrán-Rodriguez, Ricardo Rodrigo, et al. "Assessment of the hemagglutinating activity of the Porcine orthorubulavirus." Comparative immunology, microbiology and infectious diseases vol. 80 (2021): 101736. doi: https://doi.org/10.1016/j.cimid.2021.101736
Albarrán-Rodriguez RR, Castillo-Juarez H, Rivera-Benítez F, Campos-Montes GR, Espinosa B, Madrigal-Valencia TL, Jimenez ENS, Ramírez-Mendoza H. Assessment of the hemagglutinating activity of the Porcine orthorubulavirus. Comp Immunol Microbiol Infect Dis. 2021 Dec 01;80:101736. doi: 10.1016/j.cimid.2021.101736. Epub 2021 Dec 01. PMID: 34906907.
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Comp Immunol Microbiol Infect Dis. 2021 Dec 01;80:101736. doi: 10.1016/j.cimid.2021.101736. Epub 2021 Dec 01.

Assessment of the hemagglutinating activity of the Porcine orthorubulavirus.

Comparative immunology, microbiology and infectious diseases

Ricardo Rodrigo Albarrán-Rodriguez, Hector Castillo-Juarez, Francisco Rivera-Benítez, Gabriel R Campos-Montes, Blanca Espinosa, Tania Lucia Madrigal-Valencia, Erika Nayeli Salazar Jimenez, Humberto Ramírez-Mendoza

Affiliations

  1. Departamento de Microbiología e Inmunología. Facultad de Medicina, Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad Universitaria, Circuito Exterior, Coyoacán, C.P. 04510 Mexico City, Mexico. Electronic address: [email protected].
  2. Departamento de Producción Agrícola y Animal, Universidad Autónoma Metropolitana, Unidad Xochimilco, Calzada del Hueso 1100, Coyoacán, C.P. 04960 Mexico City, Mexico.
  3. Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Km. 15.5 Carretera México-Toluca, C.P. 05110 Mexico City, Mexico. Electronic address: [email protected].
  4. Departamento El Hombre y su Ambiente, Universidad Autónoma Metropolitana, Unidad Xochimilco, Calzada del Hueso 1100, Coyoacán, C.P. 04960 Mexico City, Mexico. Electronic address: [email protected].
  5. Departamento de Bioquímica, Instituto Nacional de Enfermedades Respiratorias, SSA, Calzada de Tlalpan 4502, Belisario Domínguez Secc 16, Tlalpan, C.P.14080 Mexico City, Mexico. Electronic address: [email protected].
  6. Departamento de Microbiología e Inmunología. Facultad de Medicina, Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad Universitaria, Circuito Exterior, Coyoacán, C.P. 04510 Mexico City, Mexico. Electronic address: [email protected].
  7. Departamento de Microbiología e Inmunología. Facultad de Medicina, Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad Universitaria, Circuito Exterior, Coyoacán, C.P. 04510 Mexico City, Mexico. Electronic address: [email protected].
  8. Departamento de Microbiología e Inmunología. Facultad de Medicina, Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad Universitaria, Circuito Exterior, Coyoacán, C.P. 04510 Mexico City, Mexico. Electronic address: [email protected].

PMID: 34906907 DOI: 10.1016/j.cimid.2021.101736

Abstract

Blue eye disease (BED) in pigs is caused by Porcine orthorubulavirus (PRV) of the Paramyxoviridae family. It is an endemic disease in swine production in the central region of Mexico and causes nervous signs and high mortality in suckling pigs, pneumonia in growing pigs, orchitis in boars and mummification during gestation. PRV hemagglutinates most red blood cells (RBCs) of domestic species. For serological diagnosis, the hemagglutination inhibition test is used, and in this test, guinea pig, bovine and chicken RBCs have been commonly used. In this investigation, hemagglutination with PRV was evaluated using the RBCs of seven domestic species (chicken, bovine, horse, pig, dog, guinea pig and rabbit). In the hemagglutination test, the following parameters were evaluated: temperature (25 °C and 37 °C), bottoms of the wells (V and U), erythrocyte concentration (0.5%, 0.75%, and 1%), and reading time (15, 30, 45, 60 and 90 min). Significant differences (P < 0.001) were found in most of the evaluated treatments. The best hemagglutination results were obtained with chicken, bovine and horse RBCs. The hemagglutination titer is higher (2 dilutions) when using chicken RBCs than when using bovine or horse RBCs. If chicken RBCs are used in the inhibition of hemagglutination, the test will be more sensitive, while it is more specific when bovine or horse RBCs are used. The hemagglutination readings are imprecise when using RBCs from dogs, pigs, guinea pigs and rabbits. RBCs from these species should not be used for the diagnosis or investigation of PRV.

Copyright © 2021 Elsevier Ltd. All rights reserved.

Keywords: Blue eye disease; Diagnosis; Hemagglutinating activity; Porcine orthorubulavirus

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