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Riera-Heredia N, Lutfi E, Balbuena-Pecino S, et al. The autophagy response during adipogenesis of primary cultured rainbow trout (Oncorhynchus mykiss) adipocytes. Comp Biochem Physiol B Biochem Mol Biol. 2021;258:110700doi: 10.1016/j.cbpb.2021.110700.
Riera-Heredia, N., Lutfi, E., Balbuena-Pecino, S., Vélez, E. J., Dias, K., Beaumatin, F., Gutiérrez, J., Seiliez, I., Capilla, E., & Navarro, I. (2021). The autophagy response during adipogenesis of primary cultured rainbow trout (Oncorhynchus mykiss) adipocytes. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 258110700. https://doi.org/10.1016/j.cbpb.2021.110700
Riera-Heredia, Natàlia, et al. "The autophagy response during adipogenesis of primary cultured rainbow trout (Oncorhynchus mykiss) adipocytes." Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology vol. 258 (2021): 110700. doi: https://doi.org/10.1016/j.cbpb.2021.110700
Riera-Heredia N, Lutfi E, Balbuena-Pecino S, Vélez EJ, Dias K, Beaumatin F, Gutiérrez J, Seiliez I, Capilla E, Navarro I. The autophagy response during adipogenesis of primary cultured rainbow trout (Oncorhynchus mykiss) adipocytes. Comp Biochem Physiol B Biochem Mol Biol. 2021 Nov 27;258:110700. doi: 10.1016/j.cbpb.2021.110700. Epub 2021 Nov 27. PMID: 34848371.
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Comp Biochem Physiol B Biochem Mol Biol. 2021 Nov 27;258:110700. doi: 10.1016/j.cbpb.2021.110700. Epub 2021 Nov 27.

The autophagy response during adipogenesis of primary cultured rainbow trout (Oncorhynchus mykiss) adipocytes.

Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology

Natàlia Riera-Heredia, Esmail Lutfi, Sara Balbuena-Pecino, Emilio J Vélez, Karine Dias, Florian Beaumatin, Joaquim Gutiérrez, Iban Seiliez, Encarnación Capilla, Isabel Navarro

Affiliations

  1. Departament de Biologia Cel·lular, Fisiologia i Immunologia, Facultat de Biologia, Universitat de Barcelona, Barcelona 08028, Spain.
  2. Departament de Biologia Cel·lular, Fisiologia i Immunologia, Facultat de Biologia, Universitat de Barcelona, Barcelona 08028, Spain; Université de Pau et des Pays de l'Adour, E2S UPPA, INRAE, UMR1419, Nutrition Métabolisme et Aquaculture, F-64310 Saint-Pée-sur-Nivelle, France.
  3. Université de Pau et des Pays de l'Adour, E2S UPPA, INRAE, UMR1419, Nutrition Métabolisme et Aquaculture, F-64310 Saint-Pée-sur-Nivelle, France.
  4. Departament de Biologia Cel·lular, Fisiologia i Immunologia, Facultat de Biologia, Universitat de Barcelona, Barcelona 08028, Spain. Electronic address: [email protected].

PMID: 34848371 DOI: 10.1016/j.cbpb.2021.110700

Abstract

Adipogenesis is a tightly regulated process, and the involvement of autophagy has been recently proposed in mammalian models. In rainbow trout, two well-defined phases describe the development of primary cultured adipocyte cells: proliferation and differentiation. Nevertheless, information on the transcriptional profile at the onset of differentiation and the potential role of autophagy in this process is scarce. In the present study, the cells showed an early and transient induction of several adipogenic transcription factors genes' expression (i.e., cebpa and cebpb) along with the morphological changes (round shape filled with small lipid droplets) typical of the onset of adipogenesis. Then, the expression of various lipid metabolism-related genes involving the synthesis (fas), uptake (fatp1 and cd36), accumulation (plin2) and mobilization (hsl) of lipids, characteristic of the mature adipocyte, increased. In parallel, several autophagy markers (i.e., atg4b, gabarapl1 and lc3b) mirrored the expression of those adipogenic-related genes, suggesting a role of autophagy during in vitro fish adipogenesis. In this regard, the incubation of preadipocytes with lysosomal inhibitors (Bafilomycin A1 or Chloroquine), described to prevent autophagy flux, delayed the process of adipogenesis (i.e., cell remodelling), thus suggesting a possible relationship between autophagy and adipocyte differentiation in trout. Moreover, the disruption of the autophagic flux altered the expression of some key adipogenic genes such as cebpa and pparg. Overall, this study contributes to improve our knowledge on the regulation of rainbow trout adipocyte differentiation, and highlights for the first time in fish the involvement of autophagy on adipogenesis, suggesting a close-fitting connection between both processes.

Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.

Keywords: Adipocyte differentiation; Autophagic flux; Fish; LC3

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