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Détriché G, Gendron N, Philippe A, et al. Gonadotropins as novel active partners in vascular diseases: Insight from angiogenic properties and thrombotic potential of endothelial colony-forming cells. J Thromb Haemost. 2021;20(1):230-237doi: 10.1111/jth.15549.
Détriché, G., Gendron, N., Philippe, A., Gruest, M., Billoir, P., Rossi, E., Guerin, C. L., Lokajczyk, A., Brabant, S., Prié, D., Mirault, T., & Smadja, D. M. (2022). Gonadotropins as novel active partners in vascular diseases: Insight from angiogenic properties and thrombotic potential of endothelial colony-forming cells. Journal of thrombosis and haemostasis : JTH, 20(1), 230-237. https://doi.org/10.1111/jth.15549
Détriché, Grégoire, et al. "Gonadotropins as novel active partners in vascular diseases: Insight from angiogenic properties and thrombotic potential of endothelial colony-forming cells." Journal of thrombosis and haemostasis : JTH vol. 20,1 (2022): 230-237. doi: https://doi.org/10.1111/jth.15549
Détriché G, Gendron N, Philippe A, Gruest M, Billoir P, Rossi E, Guerin CL, Lokajczyk A, Brabant S, Prié D, Mirault T, Smadja DM. Gonadotropins as novel active partners in vascular diseases: Insight from angiogenic properties and thrombotic potential of endothelial colony-forming cells. J Thromb Haemost. 2022 Jan;20(1):230-237. doi: 10.1111/jth.15549. Epub 2021 Oct 29. PMID: 34623025.
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J Thromb Haemost. 2022 Jan;20(1):230-237. doi: 10.1111/jth.15549. Epub 2021 Oct 29.

Gonadotropins as novel active partners in vascular diseases: Insight from angiogenic properties and thrombotic potential of endothelial colony-forming cells.

Journal of thrombosis and haemostasis : JTH

Grégoire Détriché, Nicolas Gendron, Aurélien Philippe, Maxime Gruest, Paul Billoir, Elisa Rossi, Coralie L Guerin, Anna Lokajczyk, Séverine Brabant, Dominique Prié, Tristan Mirault, David M Smadja

Affiliations

  1. INSERM, Innovative Therapies in Haemostasis, Université de Paris, Paris, France.
  2. Biosurgical Research Lab (Carpentier Foundation), Vascular Medicine Department, Assistance Publique Hôpitaux de Paris, Centre-Université de Paris (APHP-CUP), Paris, France.
  3. Biosurgical Research Lab (Carpentier Foundation), Hematology Department, Assistance Publique Hôpitaux de Paris, Centre-Université de Paris (APHP-CUP), Paris, France.
  4. Vascular Hemostasis Unit, UNIROUEN, INSERM U1096, Rouen University Hospital, Normandie Univ, Rouen, France.
  5. Cytometry Platform, Institut Curie, Paris, France.
  6. Department of Infection and Immunity, Luxembourg Institute of Health, Strassen, Luxembourg.
  7. AP-HP, Department of Functional Explorations, Necker Enfants Malades Hospital, Paris-Centre University, Paris Cedex, France.

PMID: 34623025 DOI: 10.1111/jth.15549

Abstract

BACKGROUND: The impact of estrogen and testosterone on atherosclerotic cardiovascular disease is well known, but the role of the gonadotropins follicle-stimulating hormone (FSH), luteinizing hormone (LH), and prolactin (PRL) to some extent remain less studied.

OBJECTIVES: To explore the angiogenic potential of gonadotropins on endothelial colony-forming cells (ECFCs).

METHODS: We examined the effects of various doses of gonadotropins on ECFCs obtained from cord blood by assessing colony number, proliferation, migration, and sprouting ability. Moreover, we studied thrombin generation in ECFCs exposed to gonadotropins by performing a thrombin generation assay. Finally, we determined the levels of circulating gonadotropins in 30 men, to exclude the effect of estrogen, with lower extremity arterial disease (LEAD), in comparison with age- and sex-matched controls.

RESULTS: Exposure to FSH, LH, or PRL resulted in an increase in ECFC migration but showed no effect on proliferation or ECFC commitment from cord blood mononuclear cells. Using a three-dimensional fibrin gel assay, we showed that ECFC sprouting was significantly enhanced by gonadotropins. Exposure to FSH also increased the thrombin generation of ECFCs exposed to FSH. Finally, FSH and LH levels in men with LEAD were higher than those in controls.

CONCLUSION: Gonadotropins increase ECFC-related angiogenesis and may be involved in thrombin generation in cardiovascular disease. Gonadotropins may act as biomarkers; moreover, we hypothesize that gonadotropin-blocking strategies may be a novel interesting therapeutic approach in atherosclerotic cardiovascular disease.

© 2021 International Society on Thrombosis and Haemostasis.

Keywords: angiogenesis modulators; endothelial colony-forming cells; endothelial progenitor cells; gonadotropins; thrombosis

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