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Chanoumidou K, Hernández-Rodríguez B, Windener F, et al. One-step Reprogramming of Human Fibroblasts into Oligodendrocyte-like Cells by SOX10, OLIG2, and NKX6.2. Stem Cell Reports. 2021;16(4):771-783doi: 10.1016/j.stemcr.2021.03.001.
Chanoumidou, K., Hernández-Rodríguez, B., Windener, F., Thomas, C., Stehling, M., Mozafari, S., Albrecht, S., Ottoboni, L., Antel, J., Kim, K. P., Velychko, S., Cui, Q. L., Xu, Y. K. T., Martino, G., Winkler, J., Schöler, H. R., Baron-Van Evercooren, A., Boespflug-Tanguy, O., Vaquerizas, J. M., Ehrlich, M., & Kuhlmann, T. (2021). One-step Reprogramming of Human Fibroblasts into Oligodendrocyte-like Cells by SOX10, OLIG2, and NKX6.2. Stem cell reports, 16(4), 771-783. https://doi.org/10.1016/j.stemcr.2021.03.001
Chanoumidou, Konstantina, et al. "One-step Reprogramming of Human Fibroblasts into Oligodendrocyte-like Cells by SOX10, OLIG2, and NKX6.2." Stem cell reports vol. 16,4 (2021): 771-783. doi: https://doi.org/10.1016/j.stemcr.2021.03.001
Chanoumidou K, Hernández-Rodríguez B, Windener F, Thomas C, Stehling M, Mozafari S, Albrecht S, Ottoboni L, Antel J, Kim KP, Velychko S, Cui QL, Xu YKT, Martino G, Winkler J, Schöler HR, Baron-Van Evercooren A, Boespflug-Tanguy O, Vaquerizas JM, Ehrlich M, Kuhlmann T. One-step Reprogramming of Human Fibroblasts into Oligodendrocyte-like Cells by SOX10, OLIG2, and NKX6.2. Stem Cell Reports. 2021 Apr 13;16(4):771-783. doi: 10.1016/j.stemcr.2021.03.001. Epub 2021 Mar 25. PMID: 33770499; PMCID: PMC8072064.
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Stem Cell Reports. 2021 Apr 13;16(4):771-783. doi: 10.1016/j.stemcr.2021.03.001. Epub 2021 Mar 25.

One-step Reprogramming of Human Fibroblasts into Oligodendrocyte-like Cells by SOX10, OLIG2, and NKX6.2.

Stem cell reports

Konstantina Chanoumidou, Benjamín Hernández-Rodríguez, Farina Windener, Christian Thomas, Martin Stehling, Sabah Mozafari, Stefanie Albrecht, Linda Ottoboni, Jack Antel, Kee-Pyo Kim, Sergiy Velychko, Qiao Ling Cui, Yu Kang T Xu, Gianvito Martino, Jürgen Winkler, Hans R Schöler, Anne Baron-Van Evercooren, Odile Boespflug-Tanguy, Juan M Vaquerizas, Marc Ehrlich, Tanja Kuhlmann

Affiliations

  1. Institute of Neuropathology, University Hospital Münster, Pottkamp 2, 48149 Münster, Germany.
  2. Max Planck Institute for Molecular Biomedicine, 48149 Münster, Germany.
  3. INSERM, U1127, 75013 Paris, France; CNRS, UMR 7225, 75013 Paris, France; Sorbonne Universités UPMC Paris 06, UM-75, 75005 Paris, France; ICM-GH Pitié-Salpêtrière, 75013 Paris, France.
  4. Neuroimmunology Unit, IRCCS San Raffaele Hospital and Vita Salute San Raffaele University, Milan, Italy.
  5. Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada.
  6. The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
  7. Department of Molecular Neurology, University Hospital Erlangen, Friedrich-Alexander-Universität Erlangen- Nürnberg, Schwabachanlage 6, 91054 Erlangen, Germany.
  8. Max Planck Institute for Molecular Biomedicine, 48149 Münster, Germany; University of Münster, Medical Faculty, Domagkstrasse 3, Münster 48149, Germany.
  9. Service de Neuropédiatrie et des Maladies Métaboliques, LEUKOFRANCE, AP-HP, Hôpital Robert Debré, 75019 Paris, France; Université de Paris, UMR1141 NeuroDiderot, INSERM, 75019 Paris, France.
  10. Max Planck Institute for Molecular Biomedicine, 48149 Münster, Germany; Medical Research Council London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College London, London, UK.
  11. Institute of Neuropathology, University Hospital Münster, Pottkamp 2, 48149 Münster, Germany. Electronic address: [email protected].

PMID: 33770499 PMCID: PMC8072064 DOI: 10.1016/j.stemcr.2021.03.001

Abstract

Limited access to human oligodendrocytes impairs better understanding of oligodendrocyte pathology in myelin diseases. Here, we describe a method to robustly convert human fibroblasts directly into oligodendrocyte-like cells (dc-hiOLs), which allows evaluation of remyelination-promoting compounds and disease modeling. Ectopic expression of SOX10, OLIG2, and NKX6.2 in human fibroblasts results in rapid generation of O4

Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.

Keywords: ATAC-seq; PMD; compound screenin; direct conversion; epigenetic age; human fibroblasts; oligodendrocytes

References

  1. Handb Clin Neurol. 2018;148:701-722 - PubMed
  2. Proc Natl Acad Sci U S A. 2014 Jan 14;111(2):829-32 - PubMed
  3. Eur J Hum Genet. 2000 Nov;8(11):837-45 - PubMed
  4. Sci Rep. 2018 Nov 21;8(1):17201 - PubMed
  5. Nature. 2019 Feb;566(7745):543-547 - PubMed
  6. Elife. 2016 Sep 20;5: - PubMed
  7. Genes Dev. 2011 Nov 1;25(21):2248-53 - PubMed
  8. Cell Stem Cell. 2015 Dec 3;17(6):705-718 - PubMed
  9. Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2243-E2252 - PubMed
  10. Nat Rev Neurosci. 2017 Nov 16;18(12):753-769 - PubMed
  11. Mol Cell. 2010 May 28;38(4):576-89 - PubMed
  12. Stem Cell Reports. 2019 Dec 10;13(6):1053-1067 - PubMed
  13. Nat Methods. 2018 Sep;15(9):700-706 - PubMed
  14. Nat Rev Neurosci. 2018 Jan;19(1):49-58 - PubMed
  15. EMBO Rep. 2018 Nov;19(11): - PubMed
  16. Development. 2014 Jan;141(1):39-50 - PubMed
  17. Commun Biol. 2019 Mar 26;2:116 - PubMed
  18. Nat Biotechnol. 2013 May;31(5):426-33 - PubMed
  19. Stem Cell Reports. 2018 Feb 13;10(2):655-672 - PubMed
  20. Am J Hum Genet. 2017 Apr 6;100(4):617-634 - PubMed
  21. Stem Cell Reports. 2014 Apr 24;2(5):648-61 - PubMed
  22. Biochem Biophys Res Commun. 2020 Feb 15;: - PubMed
  23. Nature. 2008 Aug 7;454(7205):766-70 - PubMed
  24. Nature. 2015 Jun 11;522(7555):216-20 - PubMed
  25. Trends Genet. 2007 Aug;23(8):413-8 - PubMed
  26. Nature. 2013 Oct 17;502(7471):327-332 - PubMed
  27. Nat Protoc. 2015 Aug;10(8):1143-54 - PubMed
  28. Nat Biotechnol. 2013 May;31(5):434-9 - PubMed
  29. Acta Neuropathol. 2017 Sep;134(3):351-382 - PubMed
  30. Genome Biol. 2013;14(10):R115 - PubMed
  31. Elife. 2019 May 17;8: - PubMed
  32. Nat Rev Neurol. 2017 Dec;13(12):742-754 - PubMed

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