Display options
Cite
Gao B, Ma J, Li X, et al. A real-time recombinase polymerase amplification assay for fast and accurate detection of Ditylenchus destructor. Mol Cell Probes. 2021;101788doi: 10.1016/j.mcp.2021.101788.
Gao, B., Ma, J., Li, X., Wang, R., & Chen, S. (2021). A real-time recombinase polymerase amplification assay for fast and accurate detection of Ditylenchus destructor. Molecular and cellular probes, 101788. https://doi.org/10.1016/j.mcp.2021.101788
Gao, Bo, et al. "A real-time recombinase polymerase amplification assay for fast and accurate detection of Ditylenchus destructor." Molecular and cellular probes vol. (2021): 101788. doi: https://doi.org/10.1016/j.mcp.2021.101788
Gao B, Ma J, Li X, Wang R, Chen S. A real-time recombinase polymerase amplification assay for fast and accurate detection of Ditylenchus destructor. Mol Cell Probes. 2021 Dec 22;101788. doi: 10.1016/j.mcp.2021.101788. Epub 2021 Dec 22. PMID: 34954062.
Copy Download .nbib Format: NLM
Share it on

Mol Cell Probes. 2021 Dec 22;101788. doi: 10.1016/j.mcp.2021.101788. Epub 2021 Dec 22.

A real-time recombinase polymerase amplification assay for fast and accurate detection of Ditylenchus destructor.

Molecular and cellular probes

Bo Gao, Juan Ma, Xiuhua Li, Rongyan Wang, Shulong Chen

Affiliations

  1. Plant Protection Institute of Hebei Academy of Agricultural and Forestry Sciences, IPM Center of Hebei Province, Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture and Rural Affairs, Baoding, 071000, China.
  2. Plant Protection Institute of Hebei Academy of Agricultural and Forestry Sciences, IPM Center of Hebei Province, Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture and Rural Affairs, Baoding, 071000, China. Electronic address: [email protected].
  3. Plant Protection Institute of Hebei Academy of Agricultural and Forestry Sciences, IPM Center of Hebei Province, Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture and Rural Affairs, Baoding, 071000, China. Electronic address: [email protected].

PMID: 34954062 DOI: 10.1016/j.mcp.2021.101788

Abstract

Ditylenchus destructor is a plant-parasitic nematode that seriously infests sweet potato crop in China. Thus, fast and accurate detection of D. destructor in soil and plant tissue samples is of great significance. In this study, a real-time recombinase polymerase amplification (RPA) assay was developed for the rapid and accurate detection of D. destructor in various samples. The RPA assay could be easily operated and detected as low as 1/500 individual J4 nematode DNA per reaction in 20 min at 39 °C with high specificity. The assay meets the requirements of rapid detection prior to port quarantine as well as on-site real-time detection and can be applied to detect the parasite in soil and plant samples. The modified gDNA extraction method for a single nematode established in this study significantly reduced the time of detection and improved the applicability of the real-time RPA assay for on-site detection in different environments. The real-time RPA assay to detect D. destructor will be useful for epidemiological investigations in the field as well as for quarantine processes in the sweet potato and potato trade.

Copyright © 2021. Published by Elsevier Ltd.

Keywords: Detection; Ditylenchus destructor; Plant-parasitic nematodes; Real-time; Recombinase polymerase amplification

Publication Types