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Mabonga L, Masamba P, Basson AK, et al. Microscale thermophoresis analysis of the molecular interaction between small nuclear ribonucleoprotein polypeptide G and the RING finger domain of RBBP6 towards anti-cancer drug discovery. Am J Transl Res. 2021;13(11):12775-12785
Mabonga, L., Masamba, P., Basson, A. K., & Kappo, A. P. (2021). Microscale thermophoresis analysis of the molecular interaction between small nuclear ribonucleoprotein polypeptide G and the RING finger domain of RBBP6 towards anti-cancer drug discovery. American journal of translational research, 13(11), 12775-12785.
Mabonga, Lloyd, et al. "Microscale thermophoresis analysis of the molecular interaction between small nuclear ribonucleoprotein polypeptide G and the RING finger domain of RBBP6 towards anti-cancer drug discovery." American journal of translational research vol. 13,11 (2021): 12775-12785.
Mabonga L, Masamba P, Basson AK, Kappo AP. Microscale thermophoresis analysis of the molecular interaction between small nuclear ribonucleoprotein polypeptide G and the RING finger domain of RBBP6 towards anti-cancer drug discovery. Am J Transl Res. 2021 Nov 15;13(11):12775-12785. eCollection 2021. PMID: 34956492; PMCID: PMC8661184.
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Am J Transl Res. 2021 Nov 15;13(11):12775-12785. eCollection 2021.

Microscale thermophoresis analysis of the molecular interaction between small nuclear ribonucleoprotein polypeptide G and the RING finger domain of RBBP6 towards anti-cancer drug discovery.

American journal of translational research

Lloyd Mabonga, Priscilla Masamba, Albertus Kotze Basson, Abidemi Paul Kappo

Affiliations

  1. Department of Biochemistry and Microbiology, University of Zululand KwaDlangezwa 3886, South Africa.
  2. Molecular Biophysics and Structural Biology (MBSB) Group, Department of Biochemistry, Faculty of Science, University of Johannesburg, Kingsway Campus Auckland Park 2006, South Africa.

PMID: 34956492 PMCID: PMC8661184

Abstract

Regulatory core-splicing proteins are now becoming highly promising therapeutic targets for the development of anti-cancer drugs. SNRPG and RBBP6 are two good examples of regulatory core-splicing proteins involved in tumorigenesis and tumor development whose multi-functional role is primarily mediated by protein-protein interactions. Over the years, skepticism abutting from the two onco-proteins has been mounting. Suggestive evidence using yeast 2-hybrid technique observed possible involvement between SNRPG and the RING finger domain of RBBP6. However, the putative interaction remains elusive and yet to be characterized. In this study, we developed the first MST-based assay to confirm the interaction between SNRPG and the RING finger domain of RBBP6. The results demonstrated a strong binding affinity between SNRPG and the RING finger domain of RBBP6 with a K

AJTR Copyright © 2021.

Keywords: Anti-cancer drug discovery; RBBP6; RING finger; SNPRG; cancer; microscale thermophoresis

Conflict of interest statement

None.

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