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Das P, Rajesh K, Lalzawmliana V, et al. Development and Characterization of Acellular Caprine Choncal Cartilage Matrix for Tissue Engineering Applications. Cartilage. 2019;13(2):1292S-1308Sdoi: 10.1177/1947603519855769.
Das, P., Rajesh, K., Lalzawmliana, V., Bavya Devi, K., Basak, P., Lahiri, D., Kundu, B., Roy, M., & Nandi, S. K. (2021). Development and Characterization of Acellular Caprine Choncal Cartilage Matrix for Tissue Engineering Applications. Cartilage, 13(2), 1292S-1308S. https://doi.org/10.1177/1947603519855769
Das, Piyali, et al. "Development and Characterization of Acellular Caprine Choncal Cartilage Matrix for Tissue Engineering Applications." Cartilage vol. 13,2 (2021): 1292S-1308S. doi: https://doi.org/10.1177/1947603519855769
Das P, Rajesh K, Lalzawmliana V, Bavya Devi K, Basak P, Lahiri D, Kundu B, Roy M, Nandi SK. Development and Characterization of Acellular Caprine Choncal Cartilage Matrix for Tissue Engineering Applications. Cartilage. 2021 Dec;13(2):1292S-1308S. doi: 10.1177/1947603519855769. Epub 2019 Jun 19. PMID: 31215790.
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Cartilage. 2021 Dec;13(2):1292S-1308S. doi: 10.1177/1947603519855769. Epub 2019 Jun 19.

Development and Characterization of Acellular Caprine Choncal Cartilage Matrix for Tissue Engineering Applications.

Cartilage

Piyali Das, Kanike Rajesh, V Lalzawmliana, K Bavya Devi, Piyali Basak, Debrupa Lahiri, Biswanath Kundu, Mangal Roy, Samit Kumar Nandi

Affiliations

  1. School of Biosciences and Engineering, Jadavpur University, Kolkata, West Bengal, India.
  2. Department of Metallurgical and Materials Engineering, Indian Institute of Technology, Roorkee, Uttarakhand, India.
  3. Department of Veterinary Surgery and Radiology, West Bengal University of Animal and Fishery Sciences, Kolkata, West Bengal, India.
  4. Department of Metallurgical and Materials Engineering, Indian Institute of Technology-Kharagpur, Kharagpur, West Bengal, India.
  5. Bioceramics and Coating Division, CSIR-Central Glass and Ceramic Research Institute, Kolkata, West Bengal, India.

PMID: 31215790 DOI: 10.1177/1947603519855769

Abstract

Because of poor regenerative capabilities of cartilage, reconstruction of similar rigidity and flexibility is difficult, challenging, and restricted. The aim of the present investigation was to develop cost-effective acellular xenogeneic biomaterial as cartilage substitution. Two novel biometrics have been developed using different chemical processes (Na-deoxycholate + SDS and GndHCl + NaOH) to decellularize caprine (goat) ear cartilage and further extensively characterized before preclinical investigation. Complete cell removal was ascertained by hematoxylin and eosin staining followed by DNA estimation. No adverse effect on extracellular matrix (ECM) was found by quantifying collagen and sulfated glycosaminoglycans (sGAG) content as well as collagen, sGAG and elastin staining. Results showed no drastic changes in ECM structure apart from desired sGAG loss. Scanning electron microscopy images confirmed cellular loss and unaltered orientation. Nano-indentation study on cartilage matrices indicated interesting output showing better results among decellularized groups. Increased elastic modulus and hardness indicated better stiffness and more active energy dissipation mechanism due to decellularization. Fluid uptake and retention property remained unchanged after decellularization as analyzed by swelling behavior study. Additionally, acellular materials were confirmed to be nonreactive and nonhemolytic as assessed by

Keywords: cartilage; decellularization; extracellular matrix; nanoindentation; reconstruction; xenogeneic materials

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