Display options
Cite
Murphy A, Barich D, Fennessy MS, et al. An Ohio State Scenic River Shows Elevated Antibiotic Resistance Genes, Including Acinetobacter Tetracycline and Macrolide Resistance, Downstream of Wastewater Treatment Plant Effluent. Microbiol Spectr. 2021;9(2):e0094121doi: 10.1128/Spectrum.00941-21.
Murphy, A., Barich, D., Fennessy, M. S., & Slonczewski, J. L. (2021). An Ohio State Scenic River Shows Elevated Antibiotic Resistance Genes, Including Acinetobacter Tetracycline and Macrolide Resistance, Downstream of Wastewater Treatment Plant Effluent. Microbiology spectrum, 9(2), e0094121. https://doi.org/10.1128/Spectrum.00941-21
Murphy, April, et al. "An Ohio State Scenic River Shows Elevated Antibiotic Resistance Genes, Including Acinetobacter Tetracycline and Macrolide Resistance, Downstream of Wastewater Treatment Plant Effluent." Microbiology spectrum vol. 9,2 (2021): e0094121. doi: https://doi.org/10.1128/Spectrum.00941-21
Murphy A, Barich D, Fennessy MS, Slonczewski JL. An Ohio State Scenic River Shows Elevated Antibiotic Resistance Genes, Including Acinetobacter Tetracycline and Macrolide Resistance, Downstream of Wastewater Treatment Plant Effluent. Microbiol Spectr. 2021 Oct 31;9(2):e0094121. doi: 10.1128/Spectrum.00941-21. Epub 2021 Sep 01. PMID: 34468194; PMCID: PMC8557926.
Copy Download .nbib Format: NLM
Share it on

Microbiol Spectr. 2021 Oct 31;9(2):e0094121. doi: 10.1128/Spectrum.00941-21. Epub 2021 Sep 01.

An Ohio State Scenic River Shows Elevated Antibiotic Resistance Genes, Including Acinetobacter Tetracycline and Macrolide Resistance, Downstream of Wastewater Treatment Plant Effluent.

Microbiology spectrum

April Murphy, Daniel Barich, M Siobhan Fennessy, Joan L Slonczewski

Affiliations

  1. Department of Biology, Kenyon Collegegrid.258533.a, Gambier, Ohio, USA.

PMID: 34468194 PMCID: PMC8557926 DOI: 10.1128/Spectrum.00941-21

Abstract

The entry of antibiotic resistance genes (ARGs) into aquatic systems has been documented for large municipal wastewater treatment plants (WWTPs), but there is less study of the impact of smaller plants that are situated on small rural rivers. We sampled water metagenomes for ARGs and taxa composition from the Kokosing River, a small rural river in Knox County, Ohio, which has been designated an Ohio State Scenic River for retention of natural character. Samples were obtained 1.0 km upstream, 120 m downstream, and 6.4 km downstream from the effluent release of the Mount Vernon WWTP. ARGs were identified in metagenomes using ShortBRED markers from the comprehensive antibiotic resistance database (CARD) screened against UniPROT. Through all seasons, the metagenome just downstream of the WWTP effluent showed a substantial elevation of at least 15 different ARGs, including 6 ARGs commonly associated with Acinetobacter baumannii, such as

Keywords: Acinetobacter; antibiotic resistance; metagenome; microbiome; river; wastewater

References

  1. Sci Total Environ. 2021 Jan 1;750:142266 - PubMed
  2. Mol Microbiol. 2011 Aug;81(3):705-16 - PubMed
  3. Appl Environ Microbiol. 2019 Aug 1;85(16): - PubMed
  4. FEMS Microbiol Ecol. 2020 Aug 1;96(8): - PubMed
  5. Nat Methods. 2015 Oct;12(10):902-3 - PubMed
  6. J Gen Appl Microbiol. 2014;60(2):51-8 - PubMed
  7. Antimicrob Agents Chemother. 2013 Nov;57(11):5247-57 - PubMed
  8. J Microbiol Immunol Infect. 2019 Oct;52(5):796-806 - PubMed
  9. Biomed Res Int. 2016;2016:2475067 - PubMed
  10. Nat Methods. 2012 Jun 10;9(8):811-4 - PubMed
  11. PLoS One. 2015 Mar 04;10(3):e0119403 - PubMed
  12. Sci Total Environ. 2018 Feb 1;613-614:1104-1116 - PubMed
  13. Front Cell Infect Microbiol. 2017 Mar 13;7:55 - PubMed
  14. Front Microbiol. 2012 Dec 14;3:399 - PubMed
  15. Sci Adv. 2019 Mar 27;5(3):eaau9124 - PubMed
  16. Clin Microbiol Rev. 2008 Jul;21(3):538-82 - PubMed
  17. J Microbiol Methods. 2020 Sep;176:106023 - PubMed
  18. Nucleic Acids Res. 2020 Jan 8;48(D1):D517-D525 - PubMed
  19. Bioinformatics. 2014 Aug 1;30(15):2114-20 - PubMed
  20. Environ Microbiol. 2015 Apr;17(4):913-30 - PubMed
  21. Trends Microbiol. 2017 Mar;25(3):173-181 - PubMed
  22. PLoS One. 2017 Mar 28;12(3):e0174890 - PubMed
  23. Appl Environ Microbiol. 2017 May 31;83(12): - PubMed
  24. Front Microbiol. 2018 Jan 17;8:2603 - PubMed
  25. PLoS Comput Biol. 2015 Dec 18;11(12):e1004557 - PubMed
  26. FEMS Microbiol Ecol. 2018 Apr 1;94(4): - PubMed
  27. J Hazard Mater. 2021 Feb 5;403:123689 - PubMed
  28. Nat Rev Microbiol. 2015 Jun;13(6):396 - PubMed
  29. Sci Total Environ. 2013 Mar 1;447:345-60 - PubMed
  30. J Antimicrob Chemother. 2005 Apr;55(4):566-9 - PubMed
  31. Environ Sci Technol. 2018 Jun 5;52(11):6113-6125 - PubMed
  32. Front Microbiol. 2020 Jun 18;11:1283 - PubMed
  33. Nat Rev Microbiol. 2018 Feb;16(2):91-102 - PubMed
  34. Trends Microbiol. 2018 Mar;26(3):220-228 - PubMed
  35. Sci Total Environ. 2009 Jun 1;407(12):3702-6 - PubMed
  36. Microbiol Mol Biol Rev. 2011 Mar;75(1):14-49 - PubMed
  37. Sci Rep. 2019 Aug 13;9(1):11764 - PubMed
  38. Environ Int. 2019 Dec;133(Pt B):105270 - PubMed
  39. Nat Rev Microbiol. 2015 May;13(5):310-7 - PubMed
  40. Water Res. 2018 Jun 1;136:131-136 - PubMed
  41. PLoS Pathog. 2011 Jul;7(7):e1002158 - PubMed
  42. Antimicrob Agents Chemother. 2017 Jul 25;61(8): - PubMed
  43. Int J Environ Res Public Health. 2017 Dec 30;15(1): - PubMed
  44. Environ Microbiol. 2017 Dec;19(12):5010-5024 - PubMed
  45. Environ Sci Technol. 2015 May 5;49(9):5771-8 - PubMed
  46. Int J Environ Health Res. 2018 Jun;28(3):315-322 - PubMed
  47. Water Res. 2018 Sep 1;140:261-267 - PubMed

Publication Types