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Boos MA, Grinstaff MW, Lamandé SR, et al. Contrast-Enhanced Micro-Computed Tomography for 3D Visualization and Quantification of Glycosaminoglycans in Different Cartilage Types. Cartilage. 2021;13(2):486S-494Sdoi: 10.1177/19476035211053820.
Boos, M. A., Grinstaff, M. W., Lamandé, S. R., & Stok, K. S. (2021). Contrast-Enhanced Micro-Computed Tomography for 3D Visualization and Quantification of Glycosaminoglycans in Different Cartilage Types. Cartilage, 13(2), 486S-494S. https://doi.org/10.1177/19476035211053820
Boos, Manuela A, et al. "Contrast-Enhanced Micro-Computed Tomography for 3D Visualization and Quantification of Glycosaminoglycans in Different Cartilage Types." Cartilage vol. 13,2 (2021): 486S-494S. doi: https://doi.org/10.1177/19476035211053820
Boos MA, Grinstaff MW, Lamandé SR, Stok KS. Contrast-Enhanced Micro-Computed Tomography for 3D Visualization and Quantification of Glycosaminoglycans in Different Cartilage Types. Cartilage. 2021 Dec;13(2):486S-494S. doi: 10.1177/19476035211053820. Epub 2021 Oct 25. PMID: 34696603.
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Cartilage. 2021 Dec;13(2):486S-494S. doi: 10.1177/19476035211053820. Epub 2021 Oct 25.

Contrast-Enhanced Micro-Computed Tomography for 3D Visualization and Quantification of Glycosaminoglycans in Different Cartilage Types.

Cartilage

Manuela A Boos, Mark W Grinstaff, Shireen R Lamandé, Kathryn S Stok

Affiliations

  1. Department of Biomedical Engineering, The University of Melbourne, Parkville, VIC, Australia.
  2. Departments of Chemistry and Biomedical Engineering, Boston University, Boston, MA, USA.
  3. Musculoskeletal Research, Murdoch Children's Research Institute, Parkville, VIC, Australia.
  4. Department of Paediatrics, The University of Melbourne, Parkville, VIC, Australia.

PMID: 34696603 DOI: 10.1177/19476035211053820

Abstract

OBJECTIVE: To compare CA4+-enhanced micro-computed tomography (microCT) of bovine articular, meniscal, nasal, and auricular cartilage, each of which possesses a different extracellular matrix (ECM) composition and structure.

DESIGN: The diffusion kinetics of CA4+ in different native cartilage types were assessed over 20 hours. The feasibility of CA4+-enhanced microCT to visualize and quantify glycosaminoglycans (GAGs) in these different tissues was tested using safranin-O staining and 1,9-dimethylmethylene blue assay.

RESULTS: The diffusion kinetics of CA4+ in auricular cartilage are significantly slower compared with all other cartilage types. Total GAG content per volume correlates to microCT attenuation with an R

CONCLUSIONS: CA4+-enhanced microCT enables assessment of 3-dimensiona distribution and GAG content in different types of cartilage and has promise as an

Keywords: 3D visualization; cartilage; glycosaminoglycans; micro–computed tomography; tissue engineering

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