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Haas MR, Nguyen DV, Shook BA. Recovery of Altered Diabetic Myofibroblast Heterogeneity and Gene Expression Are Associated with CD301b+ Macrophages. Biomedicines. 2021;9(12)doi: 10.3390/biomedicines9121752.
Haas, M. R., Nguyen, D. V., & Shook, B. A. (2021). Recovery of Altered Diabetic Myofibroblast Heterogeneity and Gene Expression Are Associated with CD301b+ Macrophages. Biomedicines, 9(12), . https://doi.org/10.3390/biomedicines9121752
Haas, MaryEllen R, et al. "Recovery of Altered Diabetic Myofibroblast Heterogeneity and Gene Expression Are Associated with CD301b+ Macrophages." Biomedicines vol. 9,12 (2021). doi: https://doi.org/10.3390/biomedicines9121752
Haas MR, Nguyen DV, Shook BA. Recovery of Altered Diabetic Myofibroblast Heterogeneity and Gene Expression Are Associated with CD301b+ Macrophages. Biomedicines. 2021 Nov 24;9(12). doi: 10.3390/biomedicines9121752. PMID: 34944568.
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Biomedicines. 2021 Nov 24;9(12). doi: 10.3390/biomedicines9121752.

Recovery of Altered Diabetic Myofibroblast Heterogeneity and Gene Expression Are Associated with CD301b+ Macrophages.

Biomedicines

MaryEllen R Haas, Darlene V Nguyen, Brett A Shook

Affiliations

  1. Department of Biochemistry and Molecular Medicine, School of Medicine and Health Sciences, The George Washington University, Washington, DC 20037, USA.
  2. Department of Dermatology, School of Medicine and Health Sciences, The George Washington University, Washington, DC 20037, USA.

PMID: 34944568 DOI: 10.3390/biomedicines9121752

Abstract

Diabetic wound healing is associated with impaired function and reduced numbers of myofibroblasts, a heterogeneous cell population with varying capacities to promote repair. To determine how diabetes alters myofibroblast composition, we performed flow cytometry and spatial tissue analysis of myofibroblast subsets throughout the healing process in diabetic (db/db) and control (db/+) mouse skin. We observed reduced numbers of profibrotic SCA1+; CD34+; CD26+ myofibroblasts in diabetic wounds five days after injury, with decreased expression of fibrosis-associated genes compared to myofibroblasts from db/+ mouse wounds. While the abundance of myofibroblasts remained reduced in db/db mouse wounds compared to controls, the altered myofibroblast heterogeneity and gene expression in diabetic mice was improved seven days after injury. The natural correction of myofibroblast composition and gene expression in db/db wound beds temporally corresponds with a macrophage phenotypic switch. Correlation analysis from individual wound beds revealed that wound healing in control mice is associated with CD206+ macrophages, while the rescued myofibroblast phenotypes in diabetic wounds are correlated with increased CD301b+ macrophage numbers. These data demonstrate how diabetes impacts specific subsets of myofibroblasts and indicate that signaling capable of rescuing impaired diabetic wound healing could be different from signals that regulate wound healing under nonpathological conditions.

Keywords: diabetes; macrophage; myofibroblast; wound healing

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