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Sansico F, Miroballo M, Bianco DS, et al. COVID-19 Specific Immune Markers Revealed by Single Cell Phenotypic Profiling. Biomedicines. 2021;9(12)doi: 10.3390/biomedicines9121794.
Sansico, F., Miroballo, M., Bianco, D. S., Tamiro, F., Colucci, M., Santis, E., Rossi, G., Rosati, J., Di Mauro, L., Miscio, G., Mazza, T., Vescovi, A. L., Mazzoccoli, G., Giambra, V., & On Behalf Of Css-Covid Group. (2021). COVID-19 Specific Immune Markers Revealed by Single Cell Phenotypic Profiling. Biomedicines, 9(12), . https://doi.org/10.3390/biomedicines9121794
Sansico, Francesca, et al. "COVID-19 Specific Immune Markers Revealed by Single Cell Phenotypic Profiling." Biomedicines vol. 9,12 (2021). doi: https://doi.org/10.3390/biomedicines9121794
Sansico F, Miroballo M, Bianco DS, Tamiro F, Colucci M, Santis E, Rossi G, Rosati J, Di Mauro L, Miscio G, Mazza T, Vescovi AL, Mazzoccoli G, Giambra V, On Behalf Of Css-Covid Group. COVID-19 Specific Immune Markers Revealed by Single Cell Phenotypic Profiling. Biomedicines. 2021 Nov 29;9(12). doi: 10.3390/biomedicines9121794. PMID: 34944610; PMCID: PMC8698462.
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Biomedicines. 2021 Nov 29;9(12). doi: 10.3390/biomedicines9121794.

COVID-19 Specific Immune Markers Revealed by Single Cell Phenotypic Profiling.

Biomedicines

Francesca Sansico, Mattia Miroballo, Daniele Salvatore Bianco, Francesco Tamiro, Mattia Colucci, Elisabetta De Santis, Giovanni Rossi, Jessica Rosati, Lazzaro Di Mauro, Giuseppe Miscio, Tommaso Mazza, Angelo Luigi Vescovi, Gianluigi Mazzoccoli, Vincenzo Giambra, On Behalf Of Css-Covid Group

Affiliations

  1. Institute for Stem Cell Biology, Regenerative Medicine and Innovative Therapies (ISBReMIT), Fondazione IRCCS "Casa Sollievo della Sofferenza", 71013 San Giovanni Rotondo, Italy.
  2. Bioinformatics Unit, Fondazione IRCCS Casa Sollievo della Sofferenza, 71013 San Giovanni Rotondo, Italy.
  3. Department of Hematology and Stem Cell TraNSPlant Unit, Fondazione IRCCS "Casa Sollievo della Sofferenza", 71013 San Giovanni Rotondo, Italy.
  4. Cellular Reprogramming Unit, Fondazione I.R.C.C.S. Casa Sollievo della Sofferenza, Viale dei Cappuccini, 71013 San Giovanni Rotondo, Italy.
  5. Clinical Laboratory Analysis and Transfusional Medicine, Fondazione IRCCS "Casa Sollievo della Sofferenza", 71013 San Giovanni Rotondo, Italy.
  6. Department of Medical Sciences, Division of Internal Medicine and Chronobiology Laboratory, Fondazione IRCCS Casa Sollievo della Sofferenza, 71013 San Giovanni Rotondo, Italy.

PMID: 34944610 PMCID: PMC8698462 DOI: 10.3390/biomedicines9121794

Abstract

COVID-19 is a viral infection, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and characterized by a complex inflammatory process and clinical immunophenotypes. Nowadays, several alterations of immune response within the respiratory tracts as well as at the level of the peripheral blood have been well documented. Nonetheless, their effects on COVID-19-related cell heterogeneity and disease progression are less defined. Here, we performed a single-cell RNA sequencing of about 400 transcripts relevant to immune cell function including surface markers, in mononuclear cells (PBMCs) from the peripheral blood of 50 subjects, infected with SARS-CoV-2 at the diagnosis and 27 healthy blood donors as control. We found that patients with COVID-19 exhibited an increase in COVID-specific surface markers in different subsets of immune cell composition. Interestingly, the expression of cell receptors, such as IFNGR1 and CXCR4, was reduced in response to the viral infection and associated with the inhibition of the related signaling pathways and immune functions. These results highlight novel immunoreceptors, selectively expressed in COVID-19 patients, which affect the immune functionality and are correlated with clinical outcomes.

Keywords: COVID-19; SARS-CoV-2; flow cytometry; immune cells; single-cell RNA sequencing

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