Display options
Cite
Lu G, Dong J, Fan C, et al. A coumarin-based fluorescent probe for specific detection of cysteine in the lysosome of living cells. Bioorg Chem. 2021;119:105558doi: 10.1016/j.bioorg.2021.105558.
Lu, G., Dong, J., Fan, C., Tu, Y., & Pu, S. (2021). A coumarin-based fluorescent probe for specific detection of cysteine in the lysosome of living cells. Bioorganic chemistry, 119105558. https://doi.org/10.1016/j.bioorg.2021.105558
Lu, Guowei, et al. "A coumarin-based fluorescent probe for specific detection of cysteine in the lysosome of living cells." Bioorganic chemistry vol. 119 (2021): 105558. doi: https://doi.org/10.1016/j.bioorg.2021.105558
Lu G, Dong J, Fan C, Tu Y, Pu S. A coumarin-based fluorescent probe for specific detection of cysteine in the lysosome of living cells. Bioorg Chem. 2021 Dec 13;119:105558. doi: 10.1016/j.bioorg.2021.105558. Epub 2021 Dec 13. PMID: 34922090.
Copy Download .nbib Format: NLM
Share it on

Bioorg Chem. 2021 Dec 13;119:105558. doi: 10.1016/j.bioorg.2021.105558. Epub 2021 Dec 13.

A coumarin-based fluorescent probe for specific detection of cysteine in the lysosome of living cells.

Bioorganic chemistry

Guowei Lu, Jianning Dong, Congbin Fan, Yayi Tu, Shouzhi Pu

Affiliations

  1. Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang, Jiangxi 330013, PR China.
  2. Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang, Jiangxi 330013, PR China. Electronic address: [email protected].
  3. Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang, Jiangxi 330013, PR China. Electronic address: [email protected].

PMID: 34922090 DOI: 10.1016/j.bioorg.2021.105558

Abstract

Cysteine (Cys), the only amino acid in the 20 natural amino acids that contains a reduced sulfhydryl group, plays important roles in the balance of redox homeostasis in biological systems. Lysosome is an important organelle containing a variety of hydrolases and has been proved to be the decomposition center of a variety of exogenous and endogenous macromolecular substances. In this research, a coumarin-based fluorescent probe MCA for the detection of Cys in lysosomes of living cells was developed. Due to the acrylate moiety, this probe exhibited high sensitivity (detection limit = 6.8 nM) and selectivity towards Cys superior to other analytes. Moreover, the probe was proved to be lysosome-targetable and showed good cell imaging ability and low cell toxicity.

Copyright © 2021 Elsevier Inc. All rights reserved.

Keywords: Acrylate; Cell imaging; Coumarin; Cysteine; Fluorescent probe; Lysosome-targetable

Publication Types