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Filidou E, Tarapatzi G, Spathakis M, et al. Post-Cardiac Arrest Syndrome Is Not Associated with An Early Bacterial Translocation. J Surg Res. 2021;272:51-60doi: 10.1016/j.jss.2021.11.008.
Filidou, E., Tarapatzi, G., Spathakis, M., Papadopoulos, P., Papadopoulos, C., Kandilogiannakis, L., Stavrou, G., Doumaki, E., Sioga, A., Meditskou, S., Arvanitidis, K., Papamitsou, T., Grosomanidis, V., Kolios, G., & Kotzampassi, K. (2021). Post-Cardiac Arrest Syndrome Is Not Associated with An Early Bacterial Translocation. The Journal of surgical research, 27251-60. https://doi.org/10.1016/j.jss.2021.11.008
Filidou, Eirini, et al. "Post-Cardiac Arrest Syndrome Is Not Associated with An Early Bacterial Translocation." The Journal of surgical research vol. 272 (2021): 51-60. doi: https://doi.org/10.1016/j.jss.2021.11.008
Filidou E, Tarapatzi G, Spathakis M, Papadopoulos P, Papadopoulos C, Kandilogiannakis L, Stavrou G, Doumaki E, Sioga A, Meditskou S, Arvanitidis K, Papamitsou T, Grosomanidis V, Kolios G, Kotzampassi K. Post-Cardiac Arrest Syndrome Is Not Associated with An Early Bacterial Translocation. J Surg Res. 2021 Dec 19;272:51-60. doi: 10.1016/j.jss.2021.11.008. Epub 2021 Dec 19. PMID: 34936912.
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J Surg Res. 2021 Dec 19;272:51-60. doi: 10.1016/j.jss.2021.11.008. Epub 2021 Dec 19.

Post-Cardiac Arrest Syndrome Is Not Associated with An Early Bacterial Translocation.

The Journal of surgical research

Eirini Filidou, Gesthimani Tarapatzi, Michail Spathakis, Panagiotis Papadopoulos, Charalampos Papadopoulos, Leonidas Kandilogiannakis, George Stavrou, Eleni Doumaki, Antonia Sioga, Soultana Meditskou, Konstantinos Arvanitidis, Theodora Papamitsou, Vassilios Grosomanidis, George Kolios, Katerina Kotzampassi

Affiliations

  1. Laboratory of Pharmacology, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis, Greece.
  2. Laboratory of Biochemistry, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis, Greece.
  3. Department of Surgery, Aristotle University of Thessaloniki, AHEPA Hospital, University Campus, Thessaloniki, Greece; Department of Colorectal Surgery, Addenbrooke's Hospital, Cambridge, UK.
  4. Intensive Care Unit, 424 Military Hospital, Thessaloniki, Greece.
  5. Laboratory of Histology- Embryology, Faculty of Medicine, School of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece.
  6. Department of Anaesthesiology, Aristotle University of Thessaloniki, AHEPA Hospital, University Campus, Thessaloniki, Greece.
  7. Laboratory of Pharmacology, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis, Greece. Electronic address: [email protected].
  8. Department of Surgery, Aristotle University of Thessaloniki, AHEPA Hospital, University Campus, Thessaloniki, Greece.

PMID: 34936912 DOI: 10.1016/j.jss.2021.11.008

Abstract

BACKGROUND: The aim of this study was to investigate bacterial translocation and its possible role in the development of post-resuscitation inflammatory response following Cardio-Pulmonary Resuscitation (CPR) after cardiac arrest.

METHODS: Munich female swine were employed for a model of cardiac arrest via application of electrical current. After 7 min, CPR was initiated, and animals were either successfully return to spontaneous circulation (ROSC) within 40 min or not (no-ROSC). At the end of experimental period and prior to sacrifice, samples from the intestine, mesenteric lymph nodes (MLN), liver and portal vein blood were obtained. Evaluation of inflammation and gut permeability was performed; MLN, liver and portal vein samples were analyzed for 16 s rRNA detection and cytokine mRNA expression.

RESULTS: A decreased expression of the tight junction protein Occludin, with higher levels of inflammation, greater epithelial disintegration, ulceration, loss of crypts and villi height were found in the intestines of the ROSC swine in comparison to no-ROSC. The macrophage surface antigen CD-14 staining was relatively more intense in the ROSC than in no-ROSC. Higher levels of TNF-α mRNA expression were present in the liver of the ROSC group. Finally, despite the inflammatory response and the gut mucosal alterations in ROSC group, no bacterial translocation was detected in liver, MLN and portal vein.

CONCLUSIONS: We show that resuscitation from cardiac arrest induces inflammatory response and intestinal permeability in swine 4h after resuscitation, but not a bacterial translocation. Bacterial translocation is not an early phase phenomenon but probably part of the pathophysiologic sequelae.

Copyright © 2021. Published by Elsevier Inc.

Keywords: 16 s rRNA; Bacterial translocation; Intestinal permeability; Occludin; Post-cardiac arrest syndrome; Tight junctions

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