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Hein S, Benz NI, Eisert J, et al. Comirnaty-Elicited and Convalescent Sera Recognize Different Spike Epitopes. Vaccines (Basel). 2021;9(12)doi: 10.3390/vaccines9121419.
Hein, S., Benz, N. I., Eisert, J., Herrlein, M. L., Oberle, D., Dreher, M., Stingl, J. C., Hildt, C., & Hildt, E. (2021). Comirnaty-Elicited and Convalescent Sera Recognize Different Spike Epitopes. Vaccines, 9(12), . https://doi.org/10.3390/vaccines9121419
Hein, Sascha, et al. "Comirnaty-Elicited and Convalescent Sera Recognize Different Spike Epitopes." Vaccines vol. 9,12 (2021). doi: https://doi.org/10.3390/vaccines9121419
Hein S, Benz NI, Eisert J, Herrlein ML, Oberle D, Dreher M, Stingl JC, Hildt C, Hildt E. Comirnaty-Elicited and Convalescent Sera Recognize Different Spike Epitopes. Vaccines (Basel). 2021 Dec 01;9(12). doi: 10.3390/vaccines9121419. PMID: 34960165; PMCID: PMC8708883.
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Vaccines (Basel). 2021 Dec 01;9(12). doi: 10.3390/vaccines9121419.

Comirnaty-Elicited and Convalescent Sera Recognize Different Spike Epitopes.

Vaccines

Sascha Hein, Nuka Ivalu Benz, Jonathan Eisert, Marie-Luise Herrlein, Doris Oberle, Michael Dreher, Julia C Stingl, Christoph Hildt, Eberhard Hildt

Affiliations

  1. Department of Virology, Paul-Ehrlich-Institut, Paul-Ehrlich Street 51-59, D-63225 Langen, Germany.
  2. Division of Pharmacovigilance, Paul-Ehrlich-Institut, Paul-Ehrlich Street 51-59, D-63325 Langen, Germany.
  3. Department of Pneumology and Intensive Care Medicine, RWTH Aachen University Hospital Aachen, Pauwelsstraße 30, D-52074 Aachen, Germany.
  4. Institute of Clinical Pharmacology, RWTH Aachen University Hospital Aachen, Pauwelsstraße 30, D-52074 Aachen, Germany.
  5. Main-Kinzig-Kliniken, Herzbachweg 14, D-63571 Gelnhausen, Germany.

PMID: 34960165 PMCID: PMC8708883 DOI: 10.3390/vaccines9121419

Abstract

Many of the approved SARS-CoV-2 vaccines are based on a stabilized variant of the spike protein. This raises the question of whether the immune response against the stabilized spike is identical to the immune response that is elicited by the native spike in the case of a SARS-CoV-2 infection. Using a peptide array-based approach, we analysed the binding of antibodies from Comirnaty-elicited, convalescent, and control sera to the peptides covering the spike protein. A total of 37 linear epitopes were identified. A total of 26 of these epitopes were almost exclusively recognized by the convalescent sera. Mapping these epitopes to the spike structures revealed that most of these 26 epitopes are masked in the pre-fusion structure. In particular, in the conserved central helix, three epitopes that are only exposed in the post-fusion conformation were identified. This indicates a higher spike-specific antibody diversity in convalescent sera. These differences could be relevant for the breadth of spike-specific immune response.

Keywords: SARS-CoV-2 spike; convalescent; linear epitopes; peptide array; stabilized spike protein

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