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Wang H, Li J, Qin J, et al. Investigating the cellular responses of osteosarcoma to cisplatin by confocal Raman microspectroscopy. J Photochem Photobiol B. 2021;226:112366doi: 10.1016/j.jphotobiol.2021.112366.
Wang, H., Li, J., Qin, J., Li, J., Chen, Y., Song, D., Zeng, H., & Wang, S. (2022). Investigating the cellular responses of osteosarcoma to cisplatin by confocal Raman microspectroscopy. Journal of photochemistry and photobiology. B, Biology, 226112366. https://doi.org/10.1016/j.jphotobiol.2021.112366
Wang, Haifeng, et al. "Investigating the cellular responses of osteosarcoma to cisplatin by confocal Raman microspectroscopy." Journal of photochemistry and photobiology. B, Biology vol. 226 (2022): 112366. doi: https://doi.org/10.1016/j.jphotobiol.2021.112366
Wang H, Li J, Qin J, Li J, Chen Y, Song D, Zeng H, Wang S. Investigating the cellular responses of osteosarcoma to cisplatin by confocal Raman microspectroscopy. J Photochem Photobiol B. 2022 Jan;226:112366. doi: 10.1016/j.jphotobiol.2021.112366. Epub 2021 Nov 19. PMID: 34826719.
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J Photochem Photobiol B. 2022 Jan;226:112366. doi: 10.1016/j.jphotobiol.2021.112366. Epub 2021 Nov 19.

Investigating the cellular responses of osteosarcoma to cisplatin by confocal Raman microspectroscopy.

Journal of photochemistry and photobiology. B, Biology

Haifeng Wang, Jing Li, Jie Qin, Jie Li, Yishen Chen, Dongliang Song, Haishan Zeng, Shuang Wang

Affiliations

  1. State Key Laboratory of Photon-Technology in Western China Energy, Institute of Photonics and Photon-Technology, Northwest University, Xi'an, Shaanxi 710127, China.
  2. Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, China.
  3. Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, China. Electronic address: [email protected].
  4. Imaging Unit - Integrative Oncology Department, BC Cancer Research Center, Vancouver, BC, V5Z1L3, Canada.
  5. State Key Laboratory of Photon-Technology in Western China Energy, Institute of Photonics and Photon-Technology, Northwest University, Xi'an, Shaanxi 710127, China. Electronic address: [email protected].

PMID: 34826719 DOI: 10.1016/j.jphotobiol.2021.112366

Abstract

Confocal Raman Microspectroscopy (CRM) was employed to clarify the cellular response of cisplatin in osteosarcoma (OS) cells with different dosages and incubation times. The K7M2 mouse osteosarcoma cells were treated by cisplatin in 0 μM (UT group), 20 μM (20 T group), and 40 μM (40 T group) doses for 24-h (24H group) and 48-h (48H group), respectively. Raman spectroscopy was utilized to analyze the drug induced variations of intracellular biochemical components in osteosarcoma cells. The spectral results shows that the main changes in its biochemical composition come from nucleic acids. By adopting three different kernel functions (linear, polynomial, and Gaussian radial basis function (RBF)), principal component analysis combined with support vector machine models (PCA-SVM) was built to address the spectral variations among all investigated groups. Meanwhile, multivariate curve resolution alternating least squares (MCR-ALS) was further utilized to discuss on the chemical interpretation on the acquired spectral results. Moreover, Raman spectral images, which is reconstructed by K-means cluster analysis (KCA) with point-scanned hyperspectral dataset, was applied to illustrate the drug induced compositional and morphological variations in each subcellular region. The achieved results not only prove the application potential of Raman based analytical technique in non-labeled intracellular studies, but also illustrate the detailed compositional and structural information of cisplatin induced OS cell responses from the perspective of multivariate analysis and imaging of Raman spectroscopy.

Copyright © 2021 Elsevier B.V. All rights reserved.

Keywords: Cell responses; Cisplatin; Confocal Raman microspectroscopy; K-means cluster analysis; Osteosarcoma cell; Principal component analysis-support vector machine

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