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Andreu S, Ripa I, Praena B, et al. The Valproic Acid Derivative Valpromide Inhibits Pseudorabies Virus Infection in Swine Epithelial and Mouse Neuroblastoma Cell Lines. Viruses. 2021;13(12)doi: 10.3390/v13122522.
Andreu, S., Ripa, I., Praena, B., López-Guerrero, J. A., & Bello-Morales, R. (2021). The Valproic Acid Derivative Valpromide Inhibits Pseudorabies Virus Infection in Swine Epithelial and Mouse Neuroblastoma Cell Lines. Viruses, 13(12), . https://doi.org/10.3390/v13122522
Andreu, Sabina, et al. "The Valproic Acid Derivative Valpromide Inhibits Pseudorabies Virus Infection in Swine Epithelial and Mouse Neuroblastoma Cell Lines." Viruses vol. 13,12 (2021). doi: https://doi.org/10.3390/v13122522
Andreu S, Ripa I, Praena B, López-Guerrero JA, Bello-Morales R. The Valproic Acid Derivative Valpromide Inhibits Pseudorabies Virus Infection in Swine Epithelial and Mouse Neuroblastoma Cell Lines. Viruses. 2021 Dec 15;13(12). doi: 10.3390/v13122522. PMID: 34960791.
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Viruses. 2021 Dec 15;13(12). doi: 10.3390/v13122522.

The Valproic Acid Derivative Valpromide Inhibits Pseudorabies Virus Infection in Swine Epithelial and Mouse Neuroblastoma Cell Lines.

Viruses

Sabina Andreu, Inés Ripa, Beatriz Praena, José Antonio López-Guerrero, Raquel Bello-Morales

Affiliations

  1. Departamento de Biología Molecular, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
  2. Centro de Biología Molecular Severo Ochoa, Spanish National Research Council-Universidad Autónoma de Madrid (CSIC-UAM), Cantoblanco, 28049 Madrid, Spain.
  3. MU Bond Life Sciences Center, University of Missouri, Columbia, MO 65211-7310, USA.

PMID: 34960791 DOI: 10.3390/v13122522

Abstract

Pseudorabies virus (PRV) infection of swine can produce Aujeszky's disease, which causes neurological, respiratory, and reproductive symptoms, leading to significant economic losses in the swine industry. Although humans are not the natural hosts of PRV, cases of human encephalitis and endophthalmitis caused by PRV infection have been reported between animals and workers. Currently, a lack of specific treatments and the emergence of new PRV strains against which existing vaccines do not protect makes the search for effective antiviral drugs essential. As an alternative to traditional nucleoside analogues such as acyclovir (ACV), we studied the antiviral effect of valpromide (VPD), a compound derived from valproic acid, against PRV infection in the PK15 swine cell line and the neuroblastoma cell line Neuro-2a. First, the cytotoxicity of ACV and VPD in cells was compared, demonstrating that neither compound was cytotoxic at a specific concentration range after 24 h exposure. Furthermore, the lack of direct virucidal effect of VPD outside of an infected cell environment was demonstrated. Finally, VPD was shown to have an antiviral effect on the viral production of two strains of pseudorabies virus (wild type NIA-3 and recombinant PRV-XGF) at the concentrations ranging from 0.5 to 1.5 mM, suggesting that VPD could be a suitable alternative to nucleoside analogues as an antiherpetic drug against Aujeszky's disease.

Keywords: PRV; SuHV-1; antiviral; herpesvirus; pseudorabies virus; suid herpesvirus 1; valpromide

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