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Arévalo B, Blázquez M, Serafín V, et al. Unraveling autoimmune and neurodegenerative diseases by amperometric serological detection of antibodies against aquaporin-4. Bioelectrochemistry. 2021;144:108041doi: 10.1016/j.bioelechem.2021.108041.
Arévalo, B., Blázquez, M., Serafín, V., Montero-Calle, A., Calero, M., Valverde, A., Barderas, R., Campuzano, S., Yáñez-Sedeño, P., & Pingarrón, J. M. (2021). Unraveling autoimmune and neurodegenerative diseases by amperometric serological detection of antibodies against aquaporin-4. Bioelectrochemistry (Amsterdam, Netherlands), 144108041. https://doi.org/10.1016/j.bioelechem.2021.108041
Arévalo, Beatriz, et al. "Unraveling autoimmune and neurodegenerative diseases by amperometric serological detection of antibodies against aquaporin-4." Bioelectrochemistry (Amsterdam, Netherlands) vol. 144 (2021): 108041. doi: https://doi.org/10.1016/j.bioelechem.2021.108041
Arévalo B, Blázquez M, Serafín V, Montero-Calle A, Calero M, Valverde A, Barderas R, Campuzano S, Yáñez-Sedeño P, Pingarrón JM. Unraveling autoimmune and neurodegenerative diseases by amperometric serological detection of antibodies against aquaporin-4. Bioelectrochemistry. 2021 Dec 13;144:108041. doi: 10.1016/j.bioelechem.2021.108041. Epub 2021 Dec 13. PMID: 34929532.
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Bioelectrochemistry. 2021 Dec 13;144:108041. doi: 10.1016/j.bioelechem.2021.108041. Epub 2021 Dec 13.

Unraveling autoimmune and neurodegenerative diseases by amperometric serological detection of antibodies against aquaporin-4.

Bioelectrochemistry (Amsterdam, Netherlands)

Beatriz Arévalo, Marina Blázquez, Verónica Serafín, Ana Montero-Calle, Miguel Calero, Alejandro Valverde, Rodrigo Barderas, Susana Campuzano, Paloma Yáñez-Sedeño, José M Pingarrón

Affiliations

  1. Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28014-Madrid, Spain.
  2. Chronic Disease Programme, UFIEC, Institute of Health Carlos III, Majadahonda, 28220-Madrid, Spain.
  3. Chronic Disease Programme, UFIEC, Institute of Health Carlos III, Majadahonda, 28220-Madrid, Spain; Alzheimer's Center Reina Sofía Foundation -CIEN Foundation and CIBERNED, Institute of Health Carlos III, Majadahonda, 28220-Madrid, Spain.
  4. Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28014-Madrid, Spain. Electronic address: [email protected].
  5. Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28014-Madrid, Spain. Electronic address: [email protected].

PMID: 34929532 DOI: 10.1016/j.bioelechem.2021.108041

Abstract

This work reports the first electroanalytical bioplatform to date for the determination of antibodies against aquaporin-4 (AQP4-Abs), whose serum level is considered as relevant biomarker for certain autoimmune diseases. The bioplatform relies on the use of magnetic microparticles modified with the biotinylated protein for the capture of specific antibodies. The captured IgGs are enzymatically labelled with a secondary antibody conjugated to the horseradish peroxidase (HRP) enzyme. Amperometric transduction is performed using the H

Copyright © 2021 Elsevier B.V. All rights reserved.

Keywords: Aquaporin-4 antibodies; Autoimmune diseases; Electrochemical bioplatform; Serum

Conflict of interest statement

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this pa

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