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Oliveira AF, Bastos RG, de la Torre LG. Double T-junction microfluidic and conventional dripping systems for Bacillus subtilis immobilization in calcium alginate microparticles for lipase production. Enzyme Microb Technol. 2021;154:109976doi: 10.1016/j.enzmictec.2021.109976.
Oliveira, A. F., Bastos, R. G., & de la Torre, L. G. (2021). Double T-junction microfluidic and conventional dripping systems for Bacillus subtilis immobilization in calcium alginate microparticles for lipase production. Enzyme and microbial technology, 154109976. https://doi.org/10.1016/j.enzmictec.2021.109976
Oliveira, Aline F, et al. "Double T-junction microfluidic and conventional dripping systems for Bacillus subtilis immobilization in calcium alginate microparticles for lipase production." Enzyme and microbial technology vol. 154 (2021): 109976. doi: https://doi.org/10.1016/j.enzmictec.2021.109976
Oliveira AF, Bastos RG, de la Torre LG. Double T-junction microfluidic and conventional dripping systems for Bacillus subtilis immobilization in calcium alginate microparticles for lipase production. Enzyme Microb Technol. 2021 Dec 22;154:109976. doi: 10.1016/j.enzmictec.2021.109976. Epub 2021 Dec 22. PMID: 34974340.
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Enzyme Microb Technol. 2021 Dec 22;154:109976. doi: 10.1016/j.enzmictec.2021.109976. Epub 2021 Dec 22.

Double T-junction microfluidic and conventional dripping systems for Bacillus subtilis immobilization in calcium alginate microparticles for lipase production.

Enzyme and microbial technology

Aline F Oliveira, Reinaldo G Bastos, Lucimara G de la Torre

Affiliations

  1. University of Campinas, School of Chemical Engineering, Campinas, SP, Brazil; Institute for Technological Research of State of São Paulo - IPT, São Paulo, SP, Brazil.
  2. Federal University of São Carlos, Center of Agricultural Sciences, Araras, SP, Brazil. Electronic address: [email protected].
  3. University of Campinas, School of Chemical Engineering, Campinas, SP, Brazil.

PMID: 34974340 DOI: 10.1016/j.enzmictec.2021.109976

Abstract

Bacillus subtilis immobilization in calcium alginate microparticles was investigated using two techniques: droplet microfluidics-based in T-junction geometry composed with a double droplet generation system and conventional dripping system. Alginate microparticles produced by microfluidic technology presented an average size of 68.35 µm with low polydispersity and immobilization efficiency around 86%. The cell response was evaluated in batch cultivation for 24 h, viewing lipase production compared to free cells. In this study, the batch cultivation with immobilized cells in alginate microparticles presented lipase production about 2.4 and 1.7 times higher than cultivation with cells immobilized cells by conventional technique and free cells cultivations. According to the results, this main novelty of the double T junction technique is an innovative contribution as a tool for cell immobilization on a laboratory scale, since the cultivation of immobilized cells in microparticles of small size and low polydispersity favors cell growth and increases the productivity of important metabolites of industrial biotechnology.

Copyright © 2021 Elsevier Inc. All rights reserved.

Keywords: Alginate microparticles; Bacillus subtilis; Cell immobilization; Dripping technique; Lipase; Microfluidics; T-junction droplet microfluidic

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